Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase
Fermentation of ginseng extract is limited by the low concentration of compound K (CK), a bioactive ginsenoside. In this study, a novel approach combining Aspergillus tubingensis fermentation with Aspergillus niger cellulase conversion was used to enhance CK production from high concentrations of Am...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1538031/full |
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| author | Yong-In Lee Woo-Seok Song Deok-Kun Oh |
| author_facet | Yong-In Lee Woo-Seok Song Deok-Kun Oh |
| author_sort | Yong-In Lee |
| collection | DOAJ |
| description | Fermentation of ginseng extract is limited by the low concentration of compound K (CK), a bioactive ginsenoside. In this study, a novel approach combining Aspergillus tubingensis fermentation with Aspergillus niger cellulase conversion was used to enhance CK production from high concentrations of American ginseng extract (AGE). The reaction conditions, including the feeding rate and concentrations of carbon source, enzyme type, AGE and enzyme concentrations, temperature, pH, and timing of enzyme addition, were optimized. Under optimized conditions, this combined method achieved an enhanced CK production of 8.06 g/L (13.0 mM) after 168 h, with a productivity of 48 mg/L/h. This approach led to a 2.0-fold increase in concentration and a 1.7-fold increase in productivity when compared with traditional fermentation using the same strain. The findings of this study demonstrate the synergistic effect of combining fermentation with enzyme conversion to improve CK production. |
| format | Article |
| id | doaj-art-ffbfc508d08442b88d788e5392eef481 |
| institution | Kabale University |
| issn | 2296-4185 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj-art-ffbfc508d08442b88d788e5392eef4812025-01-08T06:11:39ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852025-01-011210.3389/fbioe.2024.15380311538031Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulaseYong-In LeeWoo-Seok SongDeok-Kun OhFermentation of ginseng extract is limited by the low concentration of compound K (CK), a bioactive ginsenoside. In this study, a novel approach combining Aspergillus tubingensis fermentation with Aspergillus niger cellulase conversion was used to enhance CK production from high concentrations of American ginseng extract (AGE). The reaction conditions, including the feeding rate and concentrations of carbon source, enzyme type, AGE and enzyme concentrations, temperature, pH, and timing of enzyme addition, were optimized. Under optimized conditions, this combined method achieved an enhanced CK production of 8.06 g/L (13.0 mM) after 168 h, with a productivity of 48 mg/L/h. This approach led to a 2.0-fold increase in concentration and a 1.7-fold increase in productivity when compared with traditional fermentation using the same strain. The findings of this study demonstrate the synergistic effect of combining fermentation with enzyme conversion to improve CK production.https://www.frontiersin.org/articles/10.3389/fbioe.2024.1538031/fullAspergillus tubingensiscommercial cellulasesynergistic conversioncompound Kginseng extractfermentation |
| spellingShingle | Yong-In Lee Woo-Seok Song Deok-Kun Oh Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase Frontiers in Bioengineering and Biotechnology Aspergillus tubingensis commercial cellulase synergistic conversion compound K ginseng extract fermentation |
| title | Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase |
| title_full | Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase |
| title_fullStr | Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase |
| title_full_unstemmed | Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase |
| title_short | Enhanced production of ginsenoside compound K by synergistic conversion of fermentation with Aspergillus tubingensis and commercial cellulase |
| title_sort | enhanced production of ginsenoside compound k by synergistic conversion of fermentation with aspergillus tubingensis and commercial cellulase |
| topic | Aspergillus tubingensis commercial cellulase synergistic conversion compound K ginseng extract fermentation |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2024.1538031/full |
| work_keys_str_mv | AT yonginlee enhancedproductionofginsenosidecompoundkbysynergisticconversionoffermentationwithaspergillustubingensisandcommercialcellulase AT wooseoksong enhancedproductionofginsenosidecompoundkbysynergisticconversionoffermentationwithaspergillustubingensisandcommercialcellulase AT deokkunoh enhancedproductionofginsenosidecompoundkbysynergisticconversionoffermentationwithaspergillustubingensisandcommercialcellulase |