Cell sorters see things more clearly now
Abstract Microscopy and fluorescence‐activated cell sorting (FACS) are two of the most important tools for single‐cell phenotyping in basic and biomedical research. Microscopy provides high‐resolution snapshots of cell morphology and the inner workings of cells, while FACS isolates thousands of cell...
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| Format: | Article |
| Language: | English |
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Springer Nature
2023-02-01
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| Series: | Molecular Systems Biology |
| Online Access: | https://doi.org/10.15252/msb.202211254 |
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| _version_ | 1849331247084470272 |
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| author | Daniel Schraivogel Lars M Steinmetz |
| author_facet | Daniel Schraivogel Lars M Steinmetz |
| author_sort | Daniel Schraivogel |
| collection | DOAJ |
| description | Abstract Microscopy and fluorescence‐activated cell sorting (FACS) are two of the most important tools for single‐cell phenotyping in basic and biomedical research. Microscopy provides high‐resolution snapshots of cell morphology and the inner workings of cells, while FACS isolates thousands of cells per second using simple parameters, such as the intensity of fluorescent protein labels. Recent technologies are now combining both methods to enable the fast isolation of cells with microscopic phenotypes of interest, thereby bridging a long‐standing gap in the life sciences. In this Commentary, we discuss the technical advancements made by image‐enabled cell sorting and highlight novel experimental strategies in functional genomics and single‐cell research. |
| format | Article |
| id | doaj-art-ffbefa27a0b54fa8abae856bb7f513d3 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2023-02-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-ffbefa27a0b54fa8abae856bb7f513d32025-08-20T03:46:41ZengSpringer NatureMolecular Systems Biology1744-42922023-02-011931410.15252/msb.202211254Cell sorters see things more clearly nowDaniel Schraivogel0Lars M Steinmetz1Genome Biology Unit, European Molecular Biology Laboratory (EMBL)Genome Biology Unit, European Molecular Biology Laboratory (EMBL)Abstract Microscopy and fluorescence‐activated cell sorting (FACS) are two of the most important tools for single‐cell phenotyping in basic and biomedical research. Microscopy provides high‐resolution snapshots of cell morphology and the inner workings of cells, while FACS isolates thousands of cells per second using simple parameters, such as the intensity of fluorescent protein labels. Recent technologies are now combining both methods to enable the fast isolation of cells with microscopic phenotypes of interest, thereby bridging a long‐standing gap in the life sciences. In this Commentary, we discuss the technical advancements made by image‐enabled cell sorting and highlight novel experimental strategies in functional genomics and single‐cell research.https://doi.org/10.15252/msb.202211254 |
| spellingShingle | Daniel Schraivogel Lars M Steinmetz Cell sorters see things more clearly now Molecular Systems Biology |
| title | Cell sorters see things more clearly now |
| title_full | Cell sorters see things more clearly now |
| title_fullStr | Cell sorters see things more clearly now |
| title_full_unstemmed | Cell sorters see things more clearly now |
| title_short | Cell sorters see things more clearly now |
| title_sort | cell sorters see things more clearly now |
| url | https://doi.org/10.15252/msb.202211254 |
| work_keys_str_mv | AT danielschraivogel cellsortersseethingsmoreclearlynow AT larsmsteinmetz cellsortersseethingsmoreclearlynow |