BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid
<b>Objectives</b>: The early identification of infection-causing microorganisms through multiplex PCR panels enables prompt and targeted antibiotic therapy. This study aimed to assess the performance of the BioFire<sup>®</sup> Joint Infection Panel (BF-JIP) in analysing non-s...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-12-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/13/12/1198 |
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| author | Nicola Benvenuto Stefano Di Bella Luigi Principe Diego Luppino Jacopo Conti Venera Costantino Manuela Di Santolo Marina Busetti Roberto Luzzati Verena Zerbato |
| author_facet | Nicola Benvenuto Stefano Di Bella Luigi Principe Diego Luppino Jacopo Conti Venera Costantino Manuela Di Santolo Marina Busetti Roberto Luzzati Verena Zerbato |
| author_sort | Nicola Benvenuto |
| collection | DOAJ |
| description | <b>Objectives</b>: The early identification of infection-causing microorganisms through multiplex PCR panels enables prompt and targeted antibiotic therapy. This study aimed to assess the performance of the BioFire<sup>®</sup> Joint Infection Panel (BF-JIP) in analysing non-synovial fluid samples. <b>Methods</b>: We conducted a retrospective cohort study at Trieste University Hospital, Italy, on hospitalised adults with non-synovial fluid samples tested by both BF-JIP and traditional culture methods (November 2022–April 2024). <b>Results</b>: We evaluated 48 samples from 45 patients, including 24 abscess drainage fluids and 10 tissue samples. The BF-JIP showed high concordance (85.4%) and enhanced detection (4.3%) compared to culture methods. The BF-JIP excelled in cerebrospinal fluid (CSF) (100% accuracy and concordance) and in abscess drainage fluid (accuracy: 95.8%; concordance: 91.7%) identification and maintained high performance rates in patients under antibiotics. <b>Conclusions</b>: These findings suggest that BF-JIP is a valuable tool for accurate pathogen detection in various clinical samples, offering the additional advantage of being a rapid method. |
| format | Article |
| id | doaj-art-ff8fae84099f4c6b868f9ee792057615 |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj-art-ff8fae84099f4c6b868f9ee7920576152024-12-27T14:06:21ZengMDPI AGAntibiotics2079-63822024-12-011312119810.3390/antibiotics13121198BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial FluidNicola Benvenuto0Stefano Di Bella1Luigi Principe2Diego Luppino3Jacopo Conti4Venera Costantino5Manuela Di Santolo6Marina Busetti7Roberto Luzzati8Verena Zerbato9Infectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyClinical Department of Medical, Surgical and Health Sciences, Trieste University, 34149 Trieste, ItalyMicrobiology and Virology Unit, Great Metropolitan Hospital “Bianchi-Melacrino-Morelli”, 89128 Reggio Calabria, ItalyInfectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyInfectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, ItalyMicrobiology Unit, Trieste University Hosital (ASUGI), 34125 Trieste, ItalyMicrobiology Unit, Trieste University Hosital (ASUGI), 34125 Trieste, ItalyMicrobiology Unit, Trieste University Hosital (ASUGI), 34125 Trieste, ItalyClinical Department of Medical, Surgical and Health Sciences, Trieste University, 34149 Trieste, ItalyInfectious Diseases Unit, Trieste University Hospital (ASUGI), 34125 Trieste, Italy<b>Objectives</b>: The early identification of infection-causing microorganisms through multiplex PCR panels enables prompt and targeted antibiotic therapy. This study aimed to assess the performance of the BioFire<sup>®</sup> Joint Infection Panel (BF-JIP) in analysing non-synovial fluid samples. <b>Methods</b>: We conducted a retrospective cohort study at Trieste University Hospital, Italy, on hospitalised adults with non-synovial fluid samples tested by both BF-JIP and traditional culture methods (November 2022–April 2024). <b>Results</b>: We evaluated 48 samples from 45 patients, including 24 abscess drainage fluids and 10 tissue samples. The BF-JIP showed high concordance (85.4%) and enhanced detection (4.3%) compared to culture methods. The BF-JIP excelled in cerebrospinal fluid (CSF) (100% accuracy and concordance) and in abscess drainage fluid (accuracy: 95.8%; concordance: 91.7%) identification and maintained high performance rates in patients under antibiotics. <b>Conclusions</b>: These findings suggest that BF-JIP is a valuable tool for accurate pathogen detection in various clinical samples, offering the additional advantage of being a rapid method.https://www.mdpi.com/2079-6382/13/12/1198BioFire<sup>®</sup> Joint Infection Panelmultiplex PCR panelsdiagnosisculture-based diagnosisdiagnostic microbiology |
| spellingShingle | Nicola Benvenuto Stefano Di Bella Luigi Principe Diego Luppino Jacopo Conti Venera Costantino Manuela Di Santolo Marina Busetti Roberto Luzzati Verena Zerbato BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid Antibiotics BioFire<sup>®</sup> Joint Infection Panel multiplex PCR panels diagnosis culture-based diagnosis diagnostic microbiology |
| title | BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid |
| title_full | BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid |
| title_fullStr | BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid |
| title_full_unstemmed | BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid |
| title_short | BioFire<sup>®</sup> Joint Infection Panel for Samples Other than Synovial Fluid |
| title_sort | biofire sup r sup joint infection panel for samples other than synovial fluid |
| topic | BioFire<sup>®</sup> Joint Infection Panel multiplex PCR panels diagnosis culture-based diagnosis diagnostic microbiology |
| url | https://www.mdpi.com/2079-6382/13/12/1198 |
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