CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development
Abstract This study aimed to investigate the influence of sperm miRNAs on fertilization rates (FR) in in vitro fertilization (IVF) and to explore potential regulatory mechanisms in sperm-mediated fertilization and embryo development. Through high-throughput sequencing, we identified differentially e...
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Springer
2024-12-01
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| Series: | Molecular Biomedicine |
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| Online Access: | https://doi.org/10.1186/s43556-024-00236-5 |
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| author | Qian Sun Yanyu Li Wen Yang Wen Feng Jiayun Zhou Yijuan Cao Bei Zhang Zuobin Zhu Conghui Han |
| author_facet | Qian Sun Yanyu Li Wen Yang Wen Feng Jiayun Zhou Yijuan Cao Bei Zhang Zuobin Zhu Conghui Han |
| author_sort | Qian Sun |
| collection | DOAJ |
| description | Abstract This study aimed to investigate the influence of sperm miRNAs on fertilization rates (FR) in in vitro fertilization (IVF) and to explore potential regulatory mechanisms in sperm-mediated fertilization and embryo development. Through high-throughput sequencing, we identified differentially expressed miRNAs in sperm, with miR-133a-3p significantly upregulated in samples associated with low FR and available embryo rate (AER). Key regulatory circRNAs and mRNAs were further identified via the Starbase database, intersected with differentially expressed RNA, and analyzed through GO, KEGG, and PPI analyses. The circMYH9/miR-133a-3p/CXCR4 axis emerged as a critical regulatory network. In vitro assays using the GC-2 spd mouse spermatogenic cell line revealed that miR-133a-3p inhibited cell growth and proliferation while promoting apoptosis. circMYH9, acting as a competing endogenous RNA (ceRNA) for miR-133a-3p, modulated CXCR4 expression, enhancing GC-2 spd cell growth and inhibiting apoptosis through the miR-133a-3p/CXCR4 axis. In vivo experiments using a mouse model confirmed that circMYH9 overexpression increased IVF success rates and promoted embryo development via this axis. Mechanistically, miR-133a-3p suppresses sperm fertilization and embryo development by targeting the circMYH9/miR-133a-3p/CXCR4 axis. These findings suggest that this regulatory network could serve as a novel biomarker for assessing fertilization potential and embryo quality in clinical settings and as a potential therapeutic target to improve IVF outcomes and address infertility. This study provides valuable insights into the molecular mechanisms governing sperm function and early embryonic development. |
| format | Article |
| id | doaj-art-ff8f196b90fd4e9088b4475a07b5d44e |
| institution | Kabale University |
| issn | 2662-8651 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Springer |
| record_format | Article |
| series | Molecular Biomedicine |
| spelling | doaj-art-ff8f196b90fd4e9088b4475a07b5d44e2024-12-15T12:05:14ZengSpringerMolecular Biomedicine2662-86512024-12-015111610.1186/s43556-024-00236-5CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo developmentQian Sun0Yanyu Li1Wen Yang2Wen Feng3Jiayun Zhou4Yijuan Cao5Bei Zhang6Zuobin Zhu7Conghui Han8Suzhou Medical College, Soochow UniversitySuzhou Medical College, Soochow UniversityDepartment of Gynecology, The First Affiliated Hospital of Kangda College of Nanjing Medical UniversityDepartment of Gynecology, The First Affiliated Hospital of Kangda College of Nanjing Medical UniversityDepartment of Gynecology, Xuzhou Central HospitalDepartment of Reproductive Medicine, Xuzhou Central HospitalSuzhou Medical College, Soochow UniversityXuzhou Engineering Research Center of Medical Genetics and Transformation, Key Laboratory of Genetic Foundation and Clinical Application, Department of Genetics, Xuzhou Medical UniversityClinical Medicine Postgraduate Workstation, Soochow UniversityAbstract This study aimed to investigate the influence of sperm miRNAs on fertilization rates (FR) in in vitro fertilization (IVF) and to explore potential regulatory mechanisms in sperm-mediated fertilization and embryo development. Through high-throughput sequencing, we identified differentially expressed miRNAs in sperm, with miR-133a-3p significantly upregulated in samples associated with low FR and available embryo rate (AER). Key regulatory circRNAs and mRNAs were further identified via the Starbase database, intersected with differentially expressed RNA, and analyzed through GO, KEGG, and PPI analyses. The circMYH9/miR-133a-3p/CXCR4 axis emerged as a critical regulatory network. In vitro assays using the GC-2 spd mouse spermatogenic cell line revealed that miR-133a-3p inhibited cell growth and proliferation while promoting apoptosis. circMYH9, acting as a competing endogenous RNA (ceRNA) for miR-133a-3p, modulated CXCR4 expression, enhancing GC-2 spd cell growth and inhibiting apoptosis through the miR-133a-3p/CXCR4 axis. In vivo experiments using a mouse model confirmed that circMYH9 overexpression increased IVF success rates and promoted embryo development via this axis. Mechanistically, miR-133a-3p suppresses sperm fertilization and embryo development by targeting the circMYH9/miR-133a-3p/CXCR4 axis. These findings suggest that this regulatory network could serve as a novel biomarker for assessing fertilization potential and embryo quality in clinical settings and as a potential therapeutic target to improve IVF outcomes and address infertility. This study provides valuable insights into the molecular mechanisms governing sperm function and early embryonic development.https://doi.org/10.1186/s43556-024-00236-5SpermCircMYH9miR-133a-3pCXCR4In vitro fertilization rateEmbryo development |
| spellingShingle | Qian Sun Yanyu Li Wen Yang Wen Feng Jiayun Zhou Yijuan Cao Bei Zhang Zuobin Zhu Conghui Han CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development Molecular Biomedicine Sperm CircMYH9 miR-133a-3p CXCR4 In vitro fertilization rate Embryo development |
| title | CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development |
| title_full | CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development |
| title_fullStr | CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development |
| title_full_unstemmed | CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development |
| title_short | CircMYH9/miR-133a-3p/CXCR4 axis: a novel regulatory network in sperm fertilization and embryo development |
| title_sort | circmyh9 mir 133a 3p cxcr4 axis a novel regulatory network in sperm fertilization and embryo development |
| topic | Sperm CircMYH9 miR-133a-3p CXCR4 In vitro fertilization rate Embryo development |
| url | https://doi.org/10.1186/s43556-024-00236-5 |
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