Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum
In response to the increasing shortage of agrochemicals in developing countries, which directly impacts farmers' livelihoods, this study investigates the synthesis of silver nanoparticles (AgNPs) employ the enzymatic extract from a native strain of Fusarium oxysporum H39. These nanoparticles we...
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| Language: | English |
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Elsevier
2024-11-01
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| Series: | Plant Nano Biology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773111124000470 |
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| author | Mayra Eleonora Beltrán Pineda Luz Marina Lizarazo Forero Cesar Augusto Sierra |
| author_facet | Mayra Eleonora Beltrán Pineda Luz Marina Lizarazo Forero Cesar Augusto Sierra |
| author_sort | Mayra Eleonora Beltrán Pineda |
| collection | DOAJ |
| description | In response to the increasing shortage of agrochemicals in developing countries, which directly impacts farmers' livelihoods, this study investigates the synthesis of silver nanoparticles (AgNPs) employ the enzymatic extract from a native strain of Fusarium oxysporum H39. These nanoparticles were assessed for their antibacterial efficacy facing potato tuber soft rot caused by Pectobacterium carotovorum. The optimal conditions for AgNP synthesis were determined to be a concentration of 3 mM, a pH of 10, and a temperature of 27°C over a 24-hour period. Under these conditions, spherical nanoparticles with an average size of 12.3 ± 4.3 nm were produced. FTIR analysis indicated the presence of organic compounds on the surface of the AgNPs, as evidenced by bands corresponding to C-O, C-N, and C-C bonds. The nanoparticles demonstrated significant nanopesticide activity facing the phytopathogenic bacterium P. carotovorum, with minimum inhibitory concentrations (MICs) of 25 ppm and 50 ppm identified through microdilution and macrodilution assays, respectively. Additionally, in controlled substrate tests, preventive treatment of tubers with a 100 ppm dose of AgNPs significantly reduced the weight of macerated tissue, the primary symptom of the disease. |
| format | Article |
| id | doaj-art-ff47d5e608a94bdfa16db28e1080a3dc |
| institution | Kabale University |
| issn | 2773-1111 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Plant Nano Biology |
| spelling | doaj-art-ff47d5e608a94bdfa16db28e1080a3dc2024-12-16T05:39:18ZengElsevierPlant Nano Biology2773-11112024-11-0110100104Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorumMayra Eleonora Beltrán Pineda0Luz Marina Lizarazo Forero1Cesar Augusto Sierra2Grupo de investigación Gestión ambiental Universidad de Boyacá - Grupo de investigación en Macromoléculas UN, Grupo de investigación Biología ambiental UPTC, Tunja, Colombia; Corresponding author.Universidad Pedagógica y Tecnológica de Colombia, Grupo de investigación Biología ambiental, Tunja, ColombiaUniversidad Nacional de Colombia, Grupo de investigación en Macromoléculas, Bogotá, ColombiaIn response to the increasing shortage of agrochemicals in developing countries, which directly impacts farmers' livelihoods, this study investigates the synthesis of silver nanoparticles (AgNPs) employ the enzymatic extract from a native strain of Fusarium oxysporum H39. These nanoparticles were assessed for their antibacterial efficacy facing potato tuber soft rot caused by Pectobacterium carotovorum. The optimal conditions for AgNP synthesis were determined to be a concentration of 3 mM, a pH of 10, and a temperature of 27°C over a 24-hour period. Under these conditions, spherical nanoparticles with an average size of 12.3 ± 4.3 nm were produced. FTIR analysis indicated the presence of organic compounds on the surface of the AgNPs, as evidenced by bands corresponding to C-O, C-N, and C-C bonds. The nanoparticles demonstrated significant nanopesticide activity facing the phytopathogenic bacterium P. carotovorum, with minimum inhibitory concentrations (MICs) of 25 ppm and 50 ppm identified through microdilution and macrodilution assays, respectively. Additionally, in controlled substrate tests, preventive treatment of tubers with a 100 ppm dose of AgNPs significantly reduced the weight of macerated tissue, the primary symptom of the disease.http://www.sciencedirect.com/science/article/pii/S2773111124000470MycosynthesisFusarium oxysporumSoft rotSolanum tuberosumSilver nanoparticles |
| spellingShingle | Mayra Eleonora Beltrán Pineda Luz Marina Lizarazo Forero Cesar Augusto Sierra Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum Plant Nano Biology Mycosynthesis Fusarium oxysporum Soft rot Solanum tuberosum Silver nanoparticles |
| title | Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum |
| title_full | Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum |
| title_fullStr | Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum |
| title_full_unstemmed | Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum |
| title_short | Optimization of AgNPs production from Fusarium oxysporum H39 and its effectiveness as nanopesticides facing Pectobacterium carotovorum |
| title_sort | optimization of agnps production from fusarium oxysporum h39 and its effectiveness as nanopesticides facing pectobacterium carotovorum |
| topic | Mycosynthesis Fusarium oxysporum Soft rot Solanum tuberosum Silver nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S2773111124000470 |
| work_keys_str_mv | AT mayraeleonorabeltranpineda optimizationofagnpsproductionfromfusariumoxysporumh39anditseffectivenessasnanopesticidesfacingpectobacteriumcarotovorum AT luzmarinalizarazoforero optimizationofagnpsproductionfromfusariumoxysporumh39anditseffectivenessasnanopesticidesfacingpectobacteriumcarotovorum AT cesaraugustosierra optimizationofagnpsproductionfromfusariumoxysporumh39anditseffectivenessasnanopesticidesfacingpectobacteriumcarotovorum |