Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging
The development of novel probes featuring diverse structural motifs, with promising applications in H _2 S detection and cellular imaging remains a central focus of scientific research. This study details the synthesis of 4-methyl-N-(6-nitro-1,3-dioxo-1 H -benzo[ de ]isoquinolin-2(3 H )-yl)benzenesu...
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| Format: | Article |
| Language: | English |
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IOP Publishing
2024-01-01
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| Series: | Materials Research Express |
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| Online Access: | https://doi.org/10.1088/2053-1591/ad94d3 |
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| author | Ranjana M Namita N Kashyap Prajoy Kumar Mitra Dhanya Sunil Sudhakar Y N Sivaranjana Reddy Vennapusa Ramesh Raju Ilkka Tittonen Dinesh Upadhya |
| author_facet | Ranjana M Namita N Kashyap Prajoy Kumar Mitra Dhanya Sunil Sudhakar Y N Sivaranjana Reddy Vennapusa Ramesh Raju Ilkka Tittonen Dinesh Upadhya |
| author_sort | Ranjana M |
| collection | DOAJ |
| description | The development of novel probes featuring diverse structural motifs, with promising applications in H _2 S detection and cellular imaging remains a central focus of scientific research. This study details the synthesis of 4-methyl-N-(6-nitro-1,3-dioxo-1 H -benzo[ de ]isoquinolin-2(3 H )-yl)benzenesulfonamide (NTSH), which involves a condensation reaction between commercially available 4-nitro-1,8-naphthalic anhydride and p -toluenesulfonyl hydrazide in glacial acetic acid. The H _2 S probe exhibits detection limits of 166 μM in fluorometric, 15.08 mM in absorbance, 2.48 mM in colorimetric, and 29 nM in electrochemical studies. The versatility of NTSH in sensing endogenous H _2 S and bioimaging of HEK 293T cells highlights its potential applicability in chemical biology research. The tendency of NTSH to undergo chemical changes in the presence of H _2 S to induce noticeable optical and electrochemical responses could lay the groundwork for creating sensitive and selective sensing platforms. |
| format | Article |
| id | doaj-art-fefde68d1ebf4da5b1caab0cbbddd2fc |
| institution | Kabale University |
| issn | 2053-1591 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | IOP Publishing |
| record_format | Article |
| series | Materials Research Express |
| spelling | doaj-art-fefde68d1ebf4da5b1caab0cbbddd2fc2024-11-27T15:12:08ZengIOP PublishingMaterials Research Express2053-15912024-01-01111111510110.1088/2053-1591/ad94d3Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imagingRanjana M0Namita N Kashyap1Prajoy Kumar Mitra2Dhanya Sunil3https://orcid.org/0000-0002-0727-5125Sudhakar Y N4https://orcid.org/0000-0001-5051-4438Sivaranjana Reddy Vennapusa5https://orcid.org/0000-0002-8252-9586Ramesh Raju6Ilkka Tittonen7Dinesh Upadhya8Department of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-576104, Karnataka, IndiaCentre for Molecular Neurosciences, Kasturba Medical College, Manipal Academy of Higher Education, Manipal-576104, Karnataka, IndiaSchool of Chemistry, Indian Institute of Science Education and Research, Thiruvananthapuram-695016, Kerala, IndiaDepartment of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-576104, Karnataka, IndiaDepartment of Chemistry, Manipal Institute of Technology, Manipal Academy of Higher Education, Manipal-576104, Karnataka, IndiaSchool of Chemistry, Indian Institute of Science Education and Research, Thiruvananthapuram-695016, Kerala, IndiaDepartment of Electronics and Nanoengineering, Aalto University , PO Box 13500, FI-00076, FinlandDepartment of Electronics and Nanoengineering, Aalto University , PO Box 13500, FI-00076, FinlandCentre for Molecular Neurosciences, Kasturba Medical College, Manipal Academy of Higher Education, Manipal-576104, Karnataka, IndiaThe development of novel probes featuring diverse structural motifs, with promising applications in H _2 S detection and cellular imaging remains a central focus of scientific research. This study details the synthesis of 4-methyl-N-(6-nitro-1,3-dioxo-1 H -benzo[ de ]isoquinolin-2(3 H )-yl)benzenesulfonamide (NTSH), which involves a condensation reaction between commercially available 4-nitro-1,8-naphthalic anhydride and p -toluenesulfonyl hydrazide in glacial acetic acid. The H _2 S probe exhibits detection limits of 166 μM in fluorometric, 15.08 mM in absorbance, 2.48 mM in colorimetric, and 29 nM in electrochemical studies. The versatility of NTSH in sensing endogenous H _2 S and bioimaging of HEK 293T cells highlights its potential applicability in chemical biology research. The tendency of NTSH to undergo chemical changes in the presence of H _2 S to induce noticeable optical and electrochemical responses could lay the groundwork for creating sensitive and selective sensing platforms.https://doi.org/10.1088/2053-1591/ad94d3H2S sensorelectrochemical sensingcolorimetric sensingfluorometric sensingcellular imagingendogenous H2S |
| spellingShingle | Ranjana M Namita N Kashyap Prajoy Kumar Mitra Dhanya Sunil Sudhakar Y N Sivaranjana Reddy Vennapusa Ramesh Raju Ilkka Tittonen Dinesh Upadhya Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging Materials Research Express H2S sensor electrochemical sensing colorimetric sensing fluorometric sensing cellular imaging endogenous H2S |
| title | Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging |
| title_full | Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging |
| title_fullStr | Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging |
| title_full_unstemmed | Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging |
| title_short | Chemical synthesis and analytical profiling of NTSH: a versatile probe for hydrogen sulfide sensing and cellular imaging |
| title_sort | chemical synthesis and analytical profiling of ntsh a versatile probe for hydrogen sulfide sensing and cellular imaging |
| topic | H2S sensor electrochemical sensing colorimetric sensing fluorometric sensing cellular imaging endogenous H2S |
| url | https://doi.org/10.1088/2053-1591/ad94d3 |
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