Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration
Abstract Our aim was to investigate the potential value of immune-related miRNA signaling in predicting clinical prognosis and immunotherapy. We first identified immune-related miRNAs in lung adenocarcinoma (LUAD), and then constructed a miRNA-based risk model by lasso regression modeling. Finally,...
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| Format: | Article |
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Nature Portfolio
2025-01-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-84128-2 |
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| author | Huanzhang Yang Jingli Hua Yanxia Han Dong Chang Wenlong Zheng |
| author_facet | Huanzhang Yang Jingli Hua Yanxia Han Dong Chang Wenlong Zheng |
| author_sort | Huanzhang Yang |
| collection | DOAJ |
| description | Abstract Our aim was to investigate the potential value of immune-related miRNA signaling in predicting clinical prognosis and immunotherapy. We first identified immune-related miRNAs in lung adenocarcinoma (LUAD), and then constructed a miRNA-based risk model by lasso regression modeling. Finally, we validated our findings using RT-qPCR in serum from LUAD patients and normal patients. Weighted gene co-expression network analysis (WGCNA) was used to screen the aberrantly expressed genes associated with immune scores, and then correlation analysis and prognostic analysis were used to identify and immune-associated miRNAs, and lasso-cox regression was used to construct an immune-associated 5-miRNA model. Risk score as an independent prognostic factor could accurately predict the prognosis of LUAD patients. Immunotherapy analysis revealed that patients with low-risk scores benefited more from anti-PD-1 and CTLA-4 therapy. Experimental validation showed that only miRNA-200b-3p was significantly differentially expressed in 91 cases of clinically collected cancer tissues and normal tissue serum. We constructed a 5-miRNA model that can be used for risk stratification of LUAD patients. Targeted therapy against miRNA-200b-3p is expected to be a prospective new strategy for the clinical treatment of LUAD. |
| format | Article |
| id | doaj-art-fefc357d581c42cab5cbf0d13d173ff1 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-fefc357d581c42cab5cbf0d13d173ff12025-01-05T12:20:36ZengNature PortfolioScientific Reports2045-23222025-01-0115111110.1038/s41598-024-84128-2Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltrationHuanzhang Yang0Jingli Hua1Yanxia Han2Dong Chang3Wenlong Zheng4Department of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical CenterDepartment of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical CenterDepartment of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical CenterDepartment of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical CenterDepartment of Clinical Laboratory, Shanghai Pudong Hospital, Fudan University Pudong Medical CenterAbstract Our aim was to investigate the potential value of immune-related miRNA signaling in predicting clinical prognosis and immunotherapy. We first identified immune-related miRNAs in lung adenocarcinoma (LUAD), and then constructed a miRNA-based risk model by lasso regression modeling. Finally, we validated our findings using RT-qPCR in serum from LUAD patients and normal patients. Weighted gene co-expression network analysis (WGCNA) was used to screen the aberrantly expressed genes associated with immune scores, and then correlation analysis and prognostic analysis were used to identify and immune-associated miRNAs, and lasso-cox regression was used to construct an immune-associated 5-miRNA model. Risk score as an independent prognostic factor could accurately predict the prognosis of LUAD patients. Immunotherapy analysis revealed that patients with low-risk scores benefited more from anti-PD-1 and CTLA-4 therapy. Experimental validation showed that only miRNA-200b-3p was significantly differentially expressed in 91 cases of clinically collected cancer tissues and normal tissue serum. We constructed a 5-miRNA model that can be used for risk stratification of LUAD patients. Targeted therapy against miRNA-200b-3p is expected to be a prospective new strategy for the clinical treatment of LUAD.https://doi.org/10.1038/s41598-024-84128-2Lung adenocarcinomamiRNA-200b-3pPrognosisImmune infiltrationBiomarker |
| spellingShingle | Huanzhang Yang Jingli Hua Yanxia Han Dong Chang Wenlong Zheng Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration Scientific Reports Lung adenocarcinoma miRNA-200b-3p Prognosis Immune infiltration Biomarker |
| title | Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration |
| title_full | Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration |
| title_fullStr | Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration |
| title_full_unstemmed | Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration |
| title_short | Development and preliminary validation of five miRNAs for lung adenocarcinoma prognostic model associated with immune infiltration |
| title_sort | development and preliminary validation of five mirnas for lung adenocarcinoma prognostic model associated with immune infiltration |
| topic | Lung adenocarcinoma miRNA-200b-3p Prognosis Immune infiltration Biomarker |
| url | https://doi.org/10.1038/s41598-024-84128-2 |
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