RETRACTED: <i>In Vitro</i> Induction of Apoptosis in Isolated Acute Myeloid Leukemia Cells: The Role of <i>Anastatica hierochuntica</i> Methanolic Extract
<i>Anastatica hierochuntica</i> L. (Cruciferae) has been known in Egyptian folk medicine as a remedy for gastrointestinal disorders, diabetes and heart diseases. Despite the wide usage, <i>A. hierochuntica</i> research provides insufficient data to support its traditional pra...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-09-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/12/9/878 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | <i>Anastatica hierochuntica</i> L. (Cruciferae) has been known in Egyptian folk medicine as a remedy for gastrointestinal disorders, diabetes and heart diseases. Despite the wide usage, <i>A. hierochuntica</i> research provides insufficient data to support its traditional practice. The cytotoxicity of <i>A. hierochuntica</i> methanolic extract was investigated on acute myeloid leukemia blasts (AML) and normal human peripheral leucocytes (NHPL). The phytochemical identification of bioactive compounds using <sup>1</sup>H-NMR and LC-ESI-MS was also performed. <i>A. hierochuntica</i> extract caused non-significant cytotoxicity on NHPL, while the cytotoxicity on AML was significant (IC<sub>50</sub>: 0.38 ± 0.02 μg/mL). The negative expression of p53, upregulation of Caspase-3 and increase in the BAX/BCL-2 ratio were reported at the protein and mRNA levels. The results suggest that <i>A. hierochuntica</i> extract induced AML cell death via the p53-independent mitochondrial intrinsic pathway and further attention should be paid to this plant as a promising natural anticancer agent. |
|---|---|
| ISSN: | 2218-1989 |