Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa
This study aimed to examine how varying concentrations (0.5%, 1.0%, 2.0%, 3.0%, and 4.0%) of nano-L-α phosphatidylcholine (nano-L-α-PC) affected the quality of post-thawed buffalo bull semen compared to the same concentrations of L-α-phosphatidylcholine (L-α-PC), while 20% egg yolk (EY) was used as...
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Egyptian Society for Animal Management
2025-01-01
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| Series: | Journal of Applied Veterinary Sciences |
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| Online Access: | https://javs.journals.ekb.eg/article_392827_6c0ef7ed3371fc35141558b0837f1e9e.pdf |
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| author | Asmaa Ghania Alaa Abdel-Ghaffar Gamal Sosa Magdy Badr Mohsen Agag Mohamed Kandiel Abdelaziz Sakr Laila Kortam |
| author_facet | Asmaa Ghania Alaa Abdel-Ghaffar Gamal Sosa Magdy Badr Mohsen Agag Mohamed Kandiel Abdelaziz Sakr Laila Kortam |
| author_sort | Asmaa Ghania |
| collection | DOAJ |
| description | This study aimed to examine how varying concentrations (0.5%, 1.0%, 2.0%, 3.0%, and 4.0%) of nano-L-α phosphatidylcholine (nano-L-α-PC) affected the quality of post-thawed buffalo bull semen compared to the same concentrations of L-α-phosphatidylcholine (L-α-PC), while 20% egg yolk (EY) was used as a control. Ejaculates (n = 72) were collected from fertile buffalo bulls (n = 12) for 6 weeks. The ejaculates were pooled and divided into 11 groups (3 aliquots per group) extended with 20% EY (Group 1 without supplement), 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% nano-L-α-PC (Groups 2–6), and 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% L-α-PC (Groups 7–11) in tris buffer. After cryopreservation procedures, semen samples were thawed at 37 °C and then evaluated for sperm kinematics, acrosomes, plasma membranes, and DNA integrities. The seminal plasma was analyzed for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione reduced (GSH), and catalase (CAT). Results demonstrated that extended semen samples containing 1.0-3.0% nano-L-α-PC and 1.0-4.0% L-α-PC showed high total motility compared to control, whereas at 0.5–4.0% of nano-L-α-PC and L-α-PC showed high progressive motility. Significant (P< 0.05) wobble levels were seen at 0.5, 1.0, and 3.0% nano-L-α-PC, and distance average path and velocity average path at 2.0% L-α-PC. Acrosome and plasma membrane integrities were markedly elevated (P< 0.0001) at 2.0% nano-L-α-PC and 4.0% L-α-PC. High DNA integrity metrics were noticed at 0.5–1.0% nano-L-α-PC, as well as 3.0% and 4% L-α-PC. Low MDA and high (SOD, GSH, and CAT) levels were observed at 2.0% nano-L-α-PC and 4.0% L-α-PC. In conclusion, the optimum concentration of nano-L-α-PC that improved semen quality was 2.0%, which is roughly equivalent to the effect of 4.0% L-α-PC. |
| format | Article |
| id | doaj-art-fe5c8f2ac00946468541f35ae6f5e7d9 |
| institution | Kabale University |
| issn | 1687-4072 2090-3308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Egyptian Society for Animal Management |
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| series | Journal of Applied Veterinary Sciences |
| spelling | doaj-art-fe5c8f2ac00946468541f35ae6f5e7d92025-01-01T19:48:26ZengEgyptian Society for Animal ManagementJournal of Applied Veterinary Sciences1687-40722090-33082025-01-01101102110.21608/javs.2024.323359.1427392827Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull SpermatozoaAsmaa Ghania0Alaa Abdel-Ghaffar1Gamal Sosa2Magdy Badr3Mohsen Agag4Mohamed Kandiel5Abdelaziz Sakr6Laila Kortam7Department of Theriogenology, Faculty of Veterinary Medicine, Benha University, Benha, EgyptTheriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt. Department of Clinical Veterinary Sciences, Faculty of Veterinary Medicine, Delta University for Science and Technology, Gamasa, Dakahlia, Egypt.Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, EgyptDepartment of Artificial Insemination and Embryo Transfer, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, Egypt.Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, Egypt.Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Benha, EgyptAnimal Production Research Institute, Agricultural Research Center (A RC), Dokki, Giza, EgyptImmunity Unit, Animal Reproduction Research Institute (ARRI), Agriculture Research Center (ARC), 12556 Haram, Giza, EgyptThis study aimed to examine how varying concentrations (0.5%, 1.0%, 2.0%, 3.0%, and 4.0%) of nano-L-α phosphatidylcholine (nano-L-α-PC) affected the quality of post-thawed buffalo bull semen compared to the same concentrations of L-α-phosphatidylcholine (L-α-PC), while 20% egg yolk (EY) was used as a control. Ejaculates (n = 72) were collected from fertile buffalo bulls (n = 12) for 6 weeks. The ejaculates were pooled and divided into 11 groups (3 aliquots per group) extended with 20% EY (Group 1 without supplement), 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% nano-L-α-PC (Groups 2–6), and 0.5%, 1.0%, 2.0%, 3.0%, and 4.0% L-α-PC (Groups 7–11) in tris buffer. After cryopreservation procedures, semen samples were thawed at 37 °C and then evaluated for sperm kinematics, acrosomes, plasma membranes, and DNA integrities. The seminal plasma was analyzed for malondialdehyde (MDA), superoxide dismutase (SOD), glutathione reduced (GSH), and catalase (CAT). Results demonstrated that extended semen samples containing 1.0-3.0% nano-L-α-PC and 1.0-4.0% L-α-PC showed high total motility compared to control, whereas at 0.5–4.0% of nano-L-α-PC and L-α-PC showed high progressive motility. Significant (P< 0.05) wobble levels were seen at 0.5, 1.0, and 3.0% nano-L-α-PC, and distance average path and velocity average path at 2.0% L-α-PC. Acrosome and plasma membrane integrities were markedly elevated (P< 0.0001) at 2.0% nano-L-α-PC and 4.0% L-α-PC. High DNA integrity metrics were noticed at 0.5–1.0% nano-L-α-PC, as well as 3.0% and 4% L-α-PC. Low MDA and high (SOD, GSH, and CAT) levels were observed at 2.0% nano-L-α-PC and 4.0% L-α-PC. In conclusion, the optimum concentration of nano-L-α-PC that improved semen quality was 2.0%, which is roughly equivalent to the effect of 4.0% L-α-PC.https://javs.journals.ekb.eg/article_392827_6c0ef7ed3371fc35141558b0837f1e9e.pdfantioxidant capacitybull semencomet assaycryopreservationnano soy lecithin |
| spellingShingle | Asmaa Ghania Alaa Abdel-Ghaffar Gamal Sosa Magdy Badr Mohsen Agag Mohamed Kandiel Abdelaziz Sakr Laila Kortam Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa Journal of Applied Veterinary Sciences antioxidant capacity bull semen comet assay cryopreservation nano soy lecithin |
| title | Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa |
| title_full | Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa |
| title_fullStr | Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa |
| title_full_unstemmed | Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa |
| title_short | Influence of Egg Yolk Substitution with Nano-L-α phosphatidylcholine on Sperm Kinetics, Lipid Peroxidation, and Antioxidant Activity of Cryopreserved Buffalo Bull Spermatozoa |
| title_sort | influence of egg yolk substitution with nano l α phosphatidylcholine on sperm kinetics lipid peroxidation and antioxidant activity of cryopreserved buffalo bull spermatozoa |
| topic | antioxidant capacity bull semen comet assay cryopreservation nano soy lecithin |
| url | https://javs.journals.ekb.eg/article_392827_6c0ef7ed3371fc35141558b0837f1e9e.pdf |
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