Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota
As a phytotherapeutic agent with historical applications in epilepsy management, <i>Acorus tatarinowii</i> Schott (ATS) remains pharmacologically enigmatic, particularly regarding its pathophysiological mechanisms. This knowledge gap significantly hinders the clinical application of ATS-...
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2025-04-01
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| author | Liang Chen Jiaxin Li Wenhui Zhang Jiepeng Wang |
| author_facet | Liang Chen Jiaxin Li Wenhui Zhang Jiepeng Wang |
| author_sort | Liang Chen |
| collection | DOAJ |
| description | As a phytotherapeutic agent with historical applications in epilepsy management, <i>Acorus tatarinowii</i> Schott (ATS) remains pharmacologically enigmatic, particularly regarding its pathophysiological mechanisms. This knowledge gap significantly hinders the clinical application of ATS-based treatments. To explore the potential of ATS in combating epileptogenesis, we utilized a pentylenetetrazole (PTZ)-induced chronic epilepsy rat model. Brain metabolomic analysis was performed by ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS). Principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were performed for screening differential metabolites. Gut microbiota composition was analyzed through 16S rRNA gene sequencing and examined using Spearman correlation analysis. The results show that oral ATS (50 mg/kg) significantly improved the seizure latency and pathology of rats with epilepsy. Ascorbate and aldarate metabolism, glycerophospholipid metabolism, arachidonic acid metabolism, and intestinal flora were crucial for ATS’s ability to counteract epilepsy. The therapeutic effects of ATS against epilepsy were investigated with brain metabolomics and gut microbiota analysis, providing the basis for further comprehensive research. |
| format | Article |
| id | doaj-art-fe3c3e30b27c451fae11cc74c0fb5d1c |
| institution | Kabale University |
| issn | 2079-7737 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | MDPI AG |
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| series | Biology |
| spelling | doaj-art-fe3c3e30b27c451fae11cc74c0fb5d1c2025-08-20T03:47:48ZengMDPI AGBiology2079-77372025-04-0114548810.3390/biology14050488Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut MicrobiotaLiang Chen0Jiaxin Li1Wenhui Zhang2Jiepeng Wang3School of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaSchool of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaSchool of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, ChinaSchool of Basic Medical Sciences, Hebei University of Chinese Medicine, Shijiazhuang 050200, ChinaAs a phytotherapeutic agent with historical applications in epilepsy management, <i>Acorus tatarinowii</i> Schott (ATS) remains pharmacologically enigmatic, particularly regarding its pathophysiological mechanisms. This knowledge gap significantly hinders the clinical application of ATS-based treatments. To explore the potential of ATS in combating epileptogenesis, we utilized a pentylenetetrazole (PTZ)-induced chronic epilepsy rat model. Brain metabolomic analysis was performed by ultra-performance liquid chromatography coupled with mass spectrometry (UPLC/MS). Principal component analysis (PCA) and orthogonal projections to latent structures-discriminant analysis (OPLS-DA) were performed for screening differential metabolites. Gut microbiota composition was analyzed through 16S rRNA gene sequencing and examined using Spearman correlation analysis. The results show that oral ATS (50 mg/kg) significantly improved the seizure latency and pathology of rats with epilepsy. Ascorbate and aldarate metabolism, glycerophospholipid metabolism, arachidonic acid metabolism, and intestinal flora were crucial for ATS’s ability to counteract epilepsy. The therapeutic effects of ATS against epilepsy were investigated with brain metabolomics and gut microbiota analysis, providing the basis for further comprehensive research.https://www.mdpi.com/2079-7737/14/5/488<i>Acorus tatarinowii</i> Schottmetabolomicsmicrobiotaepilepsy |
| spellingShingle | Liang Chen Jiaxin Li Wenhui Zhang Jiepeng Wang Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota Biology <i>Acorus tatarinowii</i> Schott metabolomics microbiota epilepsy |
| title | Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota |
| title_full | Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota |
| title_fullStr | Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota |
| title_full_unstemmed | Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota |
| title_short | Antiepileptic Effects of <i>Acorus tatarinowii</i> Schott in a Rat Model of Epilepsy: Regulation of Metabolic Axes and Gut Microbiota |
| title_sort | antiepileptic effects of i acorus tatarinowii i schott in a rat model of epilepsy regulation of metabolic axes and gut microbiota |
| topic | <i>Acorus tatarinowii</i> Schott metabolomics microbiota epilepsy |
| url | https://www.mdpi.com/2079-7737/14/5/488 |
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