Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships
This study demonstrates the feasibility of producing three polysaccharides (neutral LJP -1, acidic LJP-2 and acidic LJP-3) with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica. The three polysaccharides differed in chemical composition, molecular w...
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| Format: | Article |
| Language: | English |
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Tsinghua University Press
2024-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | https://www.sciopen.com/article/10.26599/FSHW.2022.9250183 |
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| author | Yu Liu Hongjing Dong Dongxiao Sun-Waterhouse Wenwen Li Bin Zhang Jinqian Yu Zhichang Qiu Zhenjia Zheng |
| author_facet | Yu Liu Hongjing Dong Dongxiao Sun-Waterhouse Wenwen Li Bin Zhang Jinqian Yu Zhichang Qiu Zhenjia Zheng |
| author_sort | Yu Liu |
| collection | DOAJ |
| description | This study demonstrates the feasibility of producing three polysaccharides (neutral LJP -1, acidic LJP-2 and acidic LJP-3) with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica. The three polysaccharides differed in chemical composition, molecular weight (Mw) distribution, glycosidic linkage pattern, functional groups and morphology. They exhibited excellent protective effects (in a dose-dependent manner) in lipopolysaccharide-injured RAW264.7 macrophages and CuSO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors, inflammatory proteins and cytokines in the NF-κB/MAPK signaling pathways. Their anti-inflammatory effects varied owing to their different molecular characteristics and chemical compositions. Overall, LJP-2 at 400 μg/mL was the most effective. LJP-2 consisted mainly of →5)-α-L-Araf (1→, →4)-α-L-GalpA (1→ and →2)-α-L-Rhap (1→ residues with terminal T-β-D-Glcp. Thus, honeysuckle flowers are good sources of anti-inflammatory polysaccharides, and precise fractionation enables the production of potent anti-inflammatory agents for the development of functional foods and healthcare products. |
| format | Article |
| id | doaj-art-fe3b15c7838b4fcc82afea080d16e55b |
| institution | Kabale University |
| issn | 2097-0765 2213-4530 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-fe3b15c7838b4fcc82afea080d16e55b2025-01-10T06:56:48ZengTsinghua University PressFood Science and Human Wellness2097-07652213-45302024-07-011342197220710.26599/FSHW.2022.9250183Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationshipsYu Liu0Hongjing Dong1Dongxiao Sun-Waterhouse2Wenwen Li3Bin Zhang4Jinqian Yu5Zhichang Qiu6Zhenjia Zheng7Key Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaSchool of Chemical Sciences, The University of Auckland, Private Bag 92019, New ZealandKey Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory for Applied Technology of Sophisticated Analytical Instruments of Shandong Province, Shandong Analysis and Test Center, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250014, ChinaKey Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaKey Laboratory of Food Nutrition and Health in Universities of Shandong, College of Food Science and Engineering, Shandong Agricultural University, Tai’an 271018, ChinaThis study demonstrates the feasibility of producing three polysaccharides (neutral LJP -1, acidic LJP-2 and acidic LJP-3) with significant in vitro and in vivo anti-inflammatory activities from the flowers of Lonicera japonica. The three polysaccharides differed in chemical composition, molecular weight (Mw) distribution, glycosidic linkage pattern, functional groups and morphology. They exhibited excellent protective effects (in a dose-dependent manner) in lipopolysaccharide-injured RAW264.7 macrophages and CuSO4-damaged zebrafish via reducing NO production and inhibiting the overexpressions of inflammation-related transcription factors, inflammatory proteins and cytokines in the NF-κB/MAPK signaling pathways. Their anti-inflammatory effects varied owing to their different molecular characteristics and chemical compositions. Overall, LJP-2 at 400 μg/mL was the most effective. LJP-2 consisted mainly of →5)-α-L-Araf (1→, →4)-α-L-GalpA (1→ and →2)-α-L-Rhap (1→ residues with terminal T-β-D-Glcp. Thus, honeysuckle flowers are good sources of anti-inflammatory polysaccharides, and precise fractionation enables the production of potent anti-inflammatory agents for the development of functional foods and healthcare products.https://www.sciopen.com/article/10.26599/FSHW.2022.9250183honeysuckle polysaccharidesfractionationmolecular characteristicsanti-infl ammatory propertiesstructure-function relationship |
| spellingShingle | Yu Liu Hongjing Dong Dongxiao Sun-Waterhouse Wenwen Li Bin Zhang Jinqian Yu Zhichang Qiu Zhenjia Zheng Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships Food Science and Human Wellness honeysuckle polysaccharides fractionation molecular characteristics anti-infl ammatory properties structure-function relationship |
| title | Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships |
| title_full | Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships |
| title_fullStr | Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships |
| title_full_unstemmed | Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships |
| title_short | Three anti-inflammatory polysaccharides from Lonicera japonica Thunb.: insights into the structure-function relationships |
| title_sort | three anti inflammatory polysaccharides from lonicera japonica thunb insights into the structure function relationships |
| topic | honeysuckle polysaccharides fractionation molecular characteristics anti-infl ammatory properties structure-function relationship |
| url | https://www.sciopen.com/article/10.26599/FSHW.2022.9250183 |
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