Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples
Abstract Liver cancer is globally the most frequent fatal malignancy, and its identification is critical for making clinical decisions about treatment options. Pathological diagnostics and contemporary imaging technologies provide insufficient information for tumor identification. Hydrogen peroxide...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-81411-0 |
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| author | Shan E Zahra Jawad Sibtain Ahmed Dilshad Hussain Javerya Najeeb Ayub Alam Muhammad Najam-ul-Haq Batool Fatima |
| author_facet | Shan E Zahra Jawad Sibtain Ahmed Dilshad Hussain Javerya Najeeb Ayub Alam Muhammad Najam-ul-Haq Batool Fatima |
| author_sort | Shan E Zahra Jawad |
| collection | DOAJ |
| description | Abstract Liver cancer is globally the most frequent fatal malignancy, and its identification is critical for making clinical decisions about treatment options. Pathological diagnostics and contemporary imaging technologies provide insufficient information for tumor identification. Hydrogen peroxide (H2O2), an emerging biomarker is a powerful oxidant found in the tumor microenvironment, and stimulates the invasion, proliferation, and metastasis of liver cancer cells. This study describes a medically effective and sensitive electrochemical sensor based on ascorbic acid immobilized zinc selenide nanoparticles (AsA@Zn-Se NPs) decorated on a glassy carbon electrode (GCE) for determining H2O2 in PBS and human serum samples of liver cancer patients. The morphological and structural characterization of fabricated sensor is done by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray (EDX), and scanning electron microscopy (SEM). Ascorbic acid (AsA), an antioxidant for H2O2 redox behavior, is immobilized on Zn-Se NPs to aid H2O2 detection through cyclic voltammetry (CV). The sensor exhibits a low detection limit, and board linear range of 0.49 µM and 0–70 µM, respectively. The low-cost electrochemical sensor is robust for up to 100 cycles. Elecys AFP assay results validate that increasing alpha-fetoprotein (AFP) concentration, a biomarker for liver cancer, can increase the H2O2 levels in serum samples. Therefore, the proposed sensor can be used to diagnose liver cancer in clinical settings. |
| format | Article |
| id | doaj-art-fe1f8bc867174d66a0b5e374fe69b7ab |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-fe1f8bc867174d66a0b5e374fe69b7ab2025-01-05T12:15:11ZengNature PortfolioScientific Reports2045-23222025-01-0115111310.1038/s41598-024-81411-0Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samplesShan E Zahra Jawad0Sibtain Ahmed1Dilshad Hussain2Javerya Najeeb3Ayub Alam4Muhammad Najam-ul-Haq5Batool Fatima6Department of Biochemistry, Bahauddin Zakariya UniversityDepartment of Biochemistry, Bahauddin Zakariya UniversityInternational Center for Chemical and Biological Sciences, HEJ Research Institute of Chemistry, University of KarachiDrugs Testing Laboratory, near Multan Institute of Kidney DiseaseDepartment of Chemistry, The Islamia University BahawalpurInstitute of Chemical Sciences, Bahauddin Zakariya UniversityDepartment of Biochemistry, Bahauddin Zakariya UniversityAbstract Liver cancer is globally the most frequent fatal malignancy, and its identification is critical for making clinical decisions about treatment options. Pathological diagnostics and contemporary imaging technologies provide insufficient information for tumor identification. Hydrogen peroxide (H2O2), an emerging biomarker is a powerful oxidant found in the tumor microenvironment, and stimulates the invasion, proliferation, and metastasis of liver cancer cells. This study describes a medically effective and sensitive electrochemical sensor based on ascorbic acid immobilized zinc selenide nanoparticles (AsA@Zn-Se NPs) decorated on a glassy carbon electrode (GCE) for determining H2O2 in PBS and human serum samples of liver cancer patients. The morphological and structural characterization of fabricated sensor is done by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), ultraviolet-visible (UV-Vis) spectroscopy, energy dispersive X-ray (EDX), and scanning electron microscopy (SEM). Ascorbic acid (AsA), an antioxidant for H2O2 redox behavior, is immobilized on Zn-Se NPs to aid H2O2 detection through cyclic voltammetry (CV). The sensor exhibits a low detection limit, and board linear range of 0.49 µM and 0–70 µM, respectively. The low-cost electrochemical sensor is robust for up to 100 cycles. Elecys AFP assay results validate that increasing alpha-fetoprotein (AFP) concentration, a biomarker for liver cancer, can increase the H2O2 levels in serum samples. Therefore, the proposed sensor can be used to diagnose liver cancer in clinical settings.https://doi.org/10.1038/s41598-024-81411-0CancerHydrogen peroxideAscorbic acidSeleniumElectrochemical detection |
| spellingShingle | Shan E Zahra Jawad Sibtain Ahmed Dilshad Hussain Javerya Najeeb Ayub Alam Muhammad Najam-ul-Haq Batool Fatima Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples Scientific Reports Cancer Hydrogen peroxide Ascorbic acid Selenium Electrochemical detection |
| title | Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| title_full | Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| title_fullStr | Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| title_full_unstemmed | Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| title_short | Ascorbic acid-immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| title_sort | ascorbic acid immobilized zinc selenide for electrochemical monitoring of hydrogen peroxide in liver cancer samples |
| topic | Cancer Hydrogen peroxide Ascorbic acid Selenium Electrochemical detection |
| url | https://doi.org/10.1038/s41598-024-81411-0 |
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