A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field
Abstract Globally, dental caries remains a health concern due to their complications of pain, infection, and tooth loss. The traditional dental remineralization by using fluoride is effective but limited in advanced caries and continued treatments. While Calcium phosphate is beneficial in restoring...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-05-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-99607-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849237734336495616 |
|---|---|
| author | Alaa M. Khalil Samar A. Abbassy Mona Mohy ElDin Sherif Kandil Ahmed M. El-Khatib |
| author_facet | Alaa M. Khalil Samar A. Abbassy Mona Mohy ElDin Sherif Kandil Ahmed M. El-Khatib |
| author_sort | Alaa M. Khalil |
| collection | DOAJ |
| description | Abstract Globally, dental caries remains a health concern due to their complications of pain, infection, and tooth loss. The traditional dental remineralization by using fluoride is effective but limited in advanced caries and continued treatments. While Calcium phosphate is beneficial in restoring mineral, it needs external aids to be effective. This research explores a synergistic approach to enhance tooth remineralization for a total of 72 samples by harnessing the effects of nano-chitosan, fluoride, and exposure to magnetic fields. The nano-chitosan solution is prepared using an ionic interaction method, initially without fluoride, and is subsequently mixed with fluoride at concentrations of 0.05% and 2%. The structural and morphological properties of the prepared nano-chitosan were confirmed using SEM, XRD, and FTIR. The samples were exposed to a pulsed magnetic field (PMF) of 18 mT ± 2% to assess its effect on remineralization. Demineralized teeth samples are treated by synthesized agents combined and free of exposure. Treatment efficacy is evaluated using XRD, EDX, SEM, and the Vickers microhardness test. The results showed optimal enhancement of dental enamel treated by 0.05% fluoride and ChNPs with the aid of exposure. Its morphology showed new mineral layers, likely fluorapatite, and it had the highest Ca/P ratio and maximum VHN value. These findings support the possibility of prevention of early developed lesions of teeth by this non-invasive technique with low cost. Commercially, it is suggested to assess the benefit of using PMF combined with remineralizing agents instead of high-cost materials in domestic settings. |
| format | Article |
| id | doaj-art-fdca5186c2b844c28ec50d4f43a56c1d |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-fdca5186c2b844c28ec50d4f43a56c1d2025-08-20T04:01:52ZengNature PortfolioScientific Reports2045-23222025-05-0115111610.1038/s41598-025-99607-3A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic fieldAlaa M. Khalil0Samar A. Abbassy1Mona Mohy ElDin2Sherif Kandil3Ahmed M. El-Khatib4Basic Sciences Department, Faculty of Engineering, Pharos University in AlexandriaAlexandria Dental Research CenterDental Biomaterials Department, Faculty of Dentistry, Alexandria UniversityMaterials Science Department, Institute of Graduate Studies and Research, Alexandria UniversityPhysics Department, Faculty of Science, Alexandria UniversityAbstract Globally, dental caries remains a health concern due to their complications of pain, infection, and tooth loss. The traditional dental remineralization by using fluoride is effective but limited in advanced caries and continued treatments. While Calcium phosphate is beneficial in restoring mineral, it needs external aids to be effective. This research explores a synergistic approach to enhance tooth remineralization for a total of 72 samples by harnessing the effects of nano-chitosan, fluoride, and exposure to magnetic fields. The nano-chitosan solution is prepared using an ionic interaction method, initially without fluoride, and is subsequently mixed with fluoride at concentrations of 0.05% and 2%. The structural and morphological properties of the prepared nano-chitosan were confirmed using SEM, XRD, and FTIR. The samples were exposed to a pulsed magnetic field (PMF) of 18 mT ± 2% to assess its effect on remineralization. Demineralized teeth samples are treated by synthesized agents combined and free of exposure. Treatment efficacy is evaluated using XRD, EDX, SEM, and the Vickers microhardness test. The results showed optimal enhancement of dental enamel treated by 0.05% fluoride and ChNPs with the aid of exposure. Its morphology showed new mineral layers, likely fluorapatite, and it had the highest Ca/P ratio and maximum VHN value. These findings support the possibility of prevention of early developed lesions of teeth by this non-invasive technique with low cost. Commercially, it is suggested to assess the benefit of using PMF combined with remineralizing agents instead of high-cost materials in domestic settings.https://doi.org/10.1038/s41598-025-99607-3Enamel remineralizationMagnetic fieldChitosan nanoparticlesFluorideRegenerative dentistry |
| spellingShingle | Alaa M. Khalil Samar A. Abbassy Mona Mohy ElDin Sherif Kandil Ahmed M. El-Khatib A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field Scientific Reports Enamel remineralization Magnetic field Chitosan nanoparticles Fluoride Regenerative dentistry |
| title | A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field |
| title_full | A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field |
| title_fullStr | A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field |
| title_full_unstemmed | A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field |
| title_short | A synergistic approach to tooth remineralization using nano-chitosan, fluoride, and pulsed magnetic field |
| title_sort | synergistic approach to tooth remineralization using nano chitosan fluoride and pulsed magnetic field |
| topic | Enamel remineralization Magnetic field Chitosan nanoparticles Fluoride Regenerative dentistry |
| url | https://doi.org/10.1038/s41598-025-99607-3 |
| work_keys_str_mv | AT alaamkhalil asynergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT samaraabbassy asynergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT monamohyeldin asynergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT sherifkandil asynergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT ahmedmelkhatib asynergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT alaamkhalil synergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT samaraabbassy synergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT monamohyeldin synergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT sherifkandil synergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield AT ahmedmelkhatib synergisticapproachtotoothremineralizationusingnanochitosanfluorideandpulsedmagneticfield |