rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice
Background/Objectives: The biological basis for behavioral manifestations of Alzheimer’s disease remains unclear. Emotional and behavioral alterations of Alzheimer’s disease can result in substantial caregiver burden and lack effective management. This study expands upon previous work investigating...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
|
| Series: | Brain Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-3425/14/12/1186 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846105535882985472 |
|---|---|
| author | Eric P. Kraybill Fatemeh S. Mojabi Alesha M. Heath Cierra R. Spikes Charlotte Beard M. Windy McNerney |
| author_facet | Eric P. Kraybill Fatemeh S. Mojabi Alesha M. Heath Cierra R. Spikes Charlotte Beard M. Windy McNerney |
| author_sort | Eric P. Kraybill |
| collection | DOAJ |
| description | Background/Objectives: The biological basis for behavioral manifestations of Alzheimer’s disease remains unclear. Emotional and behavioral alterations of Alzheimer’s disease can result in substantial caregiver burden and lack effective management. This study expands upon previous work investigating behavioral alterations in mice with Alzheimer’s disease and a potential treatment of increasing brain-derived neurotrophic factor (BDNF) using repetitive transcranial magnetic stimulation (rTMS). Methods: A total of 47 3xTg-AD (Alzheimer’s) and 53 B6 (wildtype) mice were administered ANA12 (an antagonist of TrkB receptor) or Vehicle (saline) and then rTMS or Sham treatment daily. After 14 days of treatments and injections, mouse behavior was assessed under various behavioral cognitive tests. Mice were then perfused, and brain samples were processed for histology and protein assays. Brain homogenates were analyzed for BDNF and its downstream signaling molecules. Results: Open field testing demonstrated that 3xTg-AD mice spent more time in the center than B6 mice. 3xTg-AD-Sham mice injected with ANA12 were the only group to travel significantly less distance than B6-ANA12-Sham or B6-Vehicle-Sham mice (<i>p</i> < 0.05), while 3xTg-AD-rTMS mice (irrespective of injection) were not significantly different from B6 mice. 3xTg-AD mice had significantly greater measured levels of BDNF and TrkB than the wild-type mice. Conclusions: Treatment of Alzheimer’s disease using rTMS positively affects elements of hypoactivity, but not all behavioral abnormalities. rTMS shifted 3xTg-AD open field behavioral test measures, generating significant differences between untreated 3xTg-AD and B6 genotypes. Despite its benefit, further investigation of rTMS as a treatment for Alzheimer’s disease as well as its biological underpinnings are needed. |
| format | Article |
| id | doaj-art-fd89583ec5354c81a955e442c1e7e4b2 |
| institution | Kabale University |
| issn | 2076-3425 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Brain Sciences |
| spelling | doaj-art-fd89583ec5354c81a955e442c1e7e4b22024-12-27T14:14:43ZengMDPI AGBrain Sciences2076-34252024-11-011412118610.3390/brainsci14121186rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD MiceEric P. Kraybill0Fatemeh S. Mojabi1Alesha M. Heath2Cierra R. Spikes3Charlotte Beard4M. Windy McNerney5Mental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USAMental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USAMental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USAMental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USAMental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USAMental Illness Research Education and Clinical Center (MIRECC), Department of Veteran Affairs, 3801 Miranda Ave, Palo Alto, CA 94304, USABackground/Objectives: The biological basis for behavioral manifestations of Alzheimer’s disease remains unclear. Emotional and behavioral alterations of Alzheimer’s disease can result in substantial caregiver burden and lack effective management. This study expands upon previous work investigating behavioral alterations in mice with Alzheimer’s disease and a potential treatment of increasing brain-derived neurotrophic factor (BDNF) using repetitive transcranial magnetic stimulation (rTMS). Methods: A total of 47 3xTg-AD (Alzheimer’s) and 53 B6 (wildtype) mice were administered ANA12 (an antagonist of TrkB receptor) or Vehicle (saline) and then rTMS or Sham treatment daily. After 14 days of treatments and injections, mouse behavior was assessed under various behavioral cognitive tests. Mice were then perfused, and brain samples were processed for histology and protein assays. Brain homogenates were analyzed for BDNF and its downstream signaling molecules. Results: Open field testing demonstrated that 3xTg-AD mice spent more time in the center than B6 mice. 3xTg-AD-Sham mice injected with ANA12 were the only group to travel significantly less distance than B6-ANA12-Sham or B6-Vehicle-Sham mice (<i>p</i> < 0.05), while 3xTg-AD-rTMS mice (irrespective of injection) were not significantly different from B6 mice. 3xTg-AD mice had significantly greater measured levels of BDNF and TrkB than the wild-type mice. Conclusions: Treatment of Alzheimer’s disease using rTMS positively affects elements of hypoactivity, but not all behavioral abnormalities. rTMS shifted 3xTg-AD open field behavioral test measures, generating significant differences between untreated 3xTg-AD and B6 genotypes. Despite its benefit, further investigation of rTMS as a treatment for Alzheimer’s disease as well as its biological underpinnings are needed.https://www.mdpi.com/2076-3425/14/12/1186Alzheimer’s diseaserTMSANA12disinhibition-like behavior |
| spellingShingle | Eric P. Kraybill Fatemeh S. Mojabi Alesha M. Heath Cierra R. Spikes Charlotte Beard M. Windy McNerney rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice Brain Sciences Alzheimer’s disease rTMS ANA12 disinhibition-like behavior |
| title | rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice |
| title_full | rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice |
| title_fullStr | rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice |
| title_full_unstemmed | rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice |
| title_short | rTMS Modulation of Behavioral and Biological Measures in 3xTg-AD Mice |
| title_sort | rtms modulation of behavioral and biological measures in 3xtg ad mice |
| topic | Alzheimer’s disease rTMS ANA12 disinhibition-like behavior |
| url | https://www.mdpi.com/2076-3425/14/12/1186 |
| work_keys_str_mv | AT ericpkraybill rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice AT fatemehsmojabi rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice AT aleshamheath rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice AT cierrarspikes rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice AT charlottebeard rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice AT mwindymcnerney rtmsmodulationofbehavioralandbiologicalmeasuresin3xtgadmice |