Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa
Background: Salvia nemorosa L., belonging to the Lamiaceae family, has been widely studied due to its diverse biological activities. The essential oil (EO) of this plant has gained significant importance in pharmaceutical sciences and biotechnology due to its remarkable therapeutic potential. Method...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2025-04-01
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| Series: | Biomedical and Biotechnology Research Journal |
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| Online Access: | https://journals.lww.com/10.4103/bbrj.bbrj_109_25 |
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| author | Saeed Mollaei Parisa Koutahi Poopak Farnia |
| author_facet | Saeed Mollaei Parisa Koutahi Poopak Farnia |
| author_sort | Saeed Mollaei |
| collection | DOAJ |
| description | Background:
Salvia nemorosa L., belonging to the Lamiaceae family, has been widely studied due to its diverse biological activities. The essential oil (EO) of this plant has gained significant importance in pharmaceutical sciences and biotechnology due to its remarkable therapeutic potential.
Methods:
This study focuses on optimizing extraction methods using ultrasonic pretreatment prior to hydrodistillation (UAHD) to enhance extraction yield. The optimization of yield was performed through Box–Behnken Design and Response Surface Methodology (RSM) using three variables: ultrasonic time, ultrasonic power, and plant/liquid ratio.
Results:
Based on the RSM results, the highest EO yield was obtained with 35.5 min of ultrasonic time, 32.8°C of ultrasonic temperature, and a plant/liquid ratio of 30/451.8 g/mL, during 3 h of hydrodistillation (HD). The results indicated that the UAHD method produced the highest EO yield (0.73%) for S. nemorosa compared to the HD method, which yielded 0.2%. The study of the biological properties, including antioxidant activity, of EOs extracted from two methods (UAHD and HD) from S. nemorosa showed that the EO obtained from the UAHD method exhibited 20.60% higher antioxidant activity compared to the oil extracted by the HD method. These results indicate the superiority of the UAHD method in preserving and enhancing the antioxidant properties of the EO.
Conclusions:
Using ultrasonic pretreatment, as an environmentally friendly method, resulted in increased EO extraction yield and improved its biological activity compared to the HD method. |
| format | Article |
| id | doaj-art-fd872f23f7b34844be99e806d1afb0d2 |
| institution | DOAJ |
| issn | 2588-9834 2588-9842 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Biomedical and Biotechnology Research Journal |
| spelling | doaj-art-fd872f23f7b34844be99e806d1afb0d22025-08-20T03:16:07ZengWolters Kluwer Medknow PublicationsBiomedical and Biotechnology Research Journal2588-98342588-98422025-04-019218819410.4103/bbrj.bbrj_109_25Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosaSaeed MollaeiParisa KoutahiPoopak FarniaBackground: Salvia nemorosa L., belonging to the Lamiaceae family, has been widely studied due to its diverse biological activities. The essential oil (EO) of this plant has gained significant importance in pharmaceutical sciences and biotechnology due to its remarkable therapeutic potential. Methods: This study focuses on optimizing extraction methods using ultrasonic pretreatment prior to hydrodistillation (UAHD) to enhance extraction yield. The optimization of yield was performed through Box–Behnken Design and Response Surface Methodology (RSM) using three variables: ultrasonic time, ultrasonic power, and plant/liquid ratio. Results: Based on the RSM results, the highest EO yield was obtained with 35.5 min of ultrasonic time, 32.8°C of ultrasonic temperature, and a plant/liquid ratio of 30/451.8 g/mL, during 3 h of hydrodistillation (HD). The results indicated that the UAHD method produced the highest EO yield (0.73%) for S. nemorosa compared to the HD method, which yielded 0.2%. The study of the biological properties, including antioxidant activity, of EOs extracted from two methods (UAHD and HD) from S. nemorosa showed that the EO obtained from the UAHD method exhibited 20.60% higher antioxidant activity compared to the oil extracted by the HD method. These results indicate the superiority of the UAHD method in preserving and enhancing the antioxidant properties of the EO. Conclusions: Using ultrasonic pretreatment, as an environmentally friendly method, resulted in increased EO extraction yield and improved its biological activity compared to the HD method.https://journals.lww.com/10.4103/bbrj.bbrj_109_25antioxidantessential oilresponse surface methodologysalvia nemorosa |
| spellingShingle | Saeed Mollaei Parisa Koutahi Poopak Farnia Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa Biomedical and Biotechnology Research Journal antioxidant essential oil response surface methodology salvia nemorosa |
| title | Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa |
| title_full | Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa |
| title_fullStr | Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa |
| title_full_unstemmed | Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa |
| title_short | Effect of Ultrasonic Pretreatment on the Yield and Antioxidant Property of Salvia nemorosa |
| title_sort | effect of ultrasonic pretreatment on the yield and antioxidant property of salvia nemorosa |
| topic | antioxidant essential oil response surface methodology salvia nemorosa |
| url | https://journals.lww.com/10.4103/bbrj.bbrj_109_25 |
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