A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer
Abstract No biomarker can effectively screen for early gastric cancer (EGC). Players in the A disintegrin and metalloproteinase (ADAM)-natural killer group 2 member D (NKG2D) receptor axis may have a role for that. As a proof-of-concept pilot study, the expression of ADAM8, ADAM9, ADAM10, ADAM12, AD...
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Nature Portfolio
2025-01-01
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| Online Access: | https://doi.org/10.1038/s41598-024-84237-y |
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| author | Sooyeon Oh Sang-Soo Lee Hoeyoung Jin Seo-Hyeon Choi Choong-Keun Cha Jooho Lee KyuBum Kwack Sang Gyun Kim Sang-Woon Choi |
| author_facet | Sooyeon Oh Sang-Soo Lee Hoeyoung Jin Seo-Hyeon Choi Choong-Keun Cha Jooho Lee KyuBum Kwack Sang Gyun Kim Sang-Woon Choi |
| author_sort | Sooyeon Oh |
| collection | DOAJ |
| description | Abstract No biomarker can effectively screen for early gastric cancer (EGC). Players in the A disintegrin and metalloproteinase (ADAM)-natural killer group 2 member D (NKG2D) receptor axis may have a role for that. As a proof-of-concept pilot study, the expression of ADAM8, ADAM9, ADAM10, ADAM12, ADAM17, and major histocompatibility complex (MHC) class I chain-related sequence A (MICA), a ligand for NKG2D, in gastric cancer was investigated in silico using The Cancer Genome Atlas (TCGA) database. Subsequently, the mRNA and protein expression levels of these markers except ADAM8 were tested in blood samples from patients with EGC and healthy controls. In the TCGA data analyses, EGC tissues (n = 57) expressed significantly higher mRNA levels of ADAM8, ADAM9, ADAM10, ADAM12, and ADAM17 than normal tissues (n = 35) (p < 0.005). In human blood sample analyses, ADAM12 (p = 0.0007), ADAM17 mRNA (p < 0.0001) and ADAM10 (p < 0.0017) protein were significantly elevated in patients with EGC (n = 27 for mRNA and n = 25 for protein) compared to the controls (n = 30 for mRNA and n = 26 for protein). Areas under the curves calculated by receiver-operating characteristic analysis for ADAM12, ADAM17 mRNA and ADAM10 protein were 0.7568 (95% confidence interval [CI]: 0.6334 to 0.8802), 0.8062 (95% CI: 0.6889 to 0.9234; p < 0.0001), and 0.8108 (95% CI: 0.6895 to 0.9320; p = 0.0001), respectively. Thus, ADAM12, ADAM17 mRNA and ADAM10 protein levels in peripheral blood could hold potential as biomarkers for screening EGC, and further investigations are required. |
| format | Article |
| id | doaj-art-fd75795a84d04d32b8dc862d829ab24e |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-fd75795a84d04d32b8dc862d829ab24e2025-01-05T12:21:15ZengNature PortfolioScientific Reports2045-23222025-01-0115111210.1038/s41598-024-84237-yA disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancerSooyeon Oh0Sang-Soo Lee1Hoeyoung Jin2Seo-Hyeon Choi3Choong-Keun Cha4Jooho Lee5KyuBum Kwack6Sang Gyun Kim7Sang-Woon Choi8Chaum Life Center, CHA University School of MedicineDepartment of Biomedical Science, College of Life Science, CHA UniversityDepartment of Biomedical Science, College of Life Science, CHA UniversityDepartment of Biomedical Science, College of Life Science, CHA UniversityChaum Life Center, CHA University School of MedicineDepartment of Gastroenterology and Hepatology, CHA Bundang Medical Center, CHA University School of MedicineDepartment of Biomedical Science, College of Life Science, CHA UniversityDivision of Gastroenterology, Department of Internal Medicine and Liver Research Institute, Seoul National University College of MedicineChaum Life Center, CHA University School of MedicineAbstract No biomarker can effectively screen for early gastric cancer (EGC). Players in the A disintegrin and metalloproteinase (ADAM)-natural killer group 2 member D (NKG2D) receptor axis may have a role for that. As a proof-of-concept pilot study, the expression of ADAM8, ADAM9, ADAM10, ADAM12, ADAM17, and major histocompatibility complex (MHC) class I chain-related sequence A (MICA), a ligand for NKG2D, in gastric cancer was investigated in silico using The Cancer Genome Atlas (TCGA) database. Subsequently, the mRNA and protein expression levels of these markers except ADAM8 were tested in blood samples from patients with EGC and healthy controls. In the TCGA data analyses, EGC tissues (n = 57) expressed significantly higher mRNA levels of ADAM8, ADAM9, ADAM10, ADAM12, and ADAM17 than normal tissues (n = 35) (p < 0.005). In human blood sample analyses, ADAM12 (p = 0.0007), ADAM17 mRNA (p < 0.0001) and ADAM10 (p < 0.0017) protein were significantly elevated in patients with EGC (n = 27 for mRNA and n = 25 for protein) compared to the controls (n = 30 for mRNA and n = 26 for protein). Areas under the curves calculated by receiver-operating characteristic analysis for ADAM12, ADAM17 mRNA and ADAM10 protein were 0.7568 (95% confidence interval [CI]: 0.6334 to 0.8802), 0.8062 (95% CI: 0.6889 to 0.9234; p < 0.0001), and 0.8108 (95% CI: 0.6895 to 0.9320; p = 0.0001), respectively. Thus, ADAM12, ADAM17 mRNA and ADAM10 protein levels in peripheral blood could hold potential as biomarkers for screening EGC, and further investigations are required.https://doi.org/10.1038/s41598-024-84237-yADAM12ADAM17ADAM10Gastric cancerBiomarker |
| spellingShingle | Sooyeon Oh Sang-Soo Lee Hoeyoung Jin Seo-Hyeon Choi Choong-Keun Cha Jooho Lee KyuBum Kwack Sang Gyun Kim Sang-Woon Choi A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer Scientific Reports ADAM12 ADAM17 ADAM10 Gastric cancer Biomarker |
| title | A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer |
| title_full | A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer |
| title_fullStr | A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer |
| title_full_unstemmed | A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer |
| title_short | A disintegrin and metallopeptidase domain (ADAM) 12, ADAM 17 mRNA and ADAM10 protein hold potential as biomarkers for detection of early gastric cancer |
| title_sort | disintegrin and metallopeptidase domain adam 12 adam 17 mrna and adam10 protein hold potential as biomarkers for detection of early gastric cancer |
| topic | ADAM12 ADAM17 ADAM10 Gastric cancer Biomarker |
| url | https://doi.org/10.1038/s41598-024-84237-y |
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