High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways

In this study, we devoted attention to the hepatoprotective functions of high Fischer ratio oligopeptides (HFOPs) from Antarctic krill (HFOPs-AK) on alcoholic liver injury of mice. The results indicated that HFOPs-AK significantly improved the pathological state of mice liver and kidney, reduced ala...

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Main Authors: Xiao-Meng Dong, Shi-Kun Suo, Yu-Mei Wang, Yu-Hui Zeng, Chang-Feng Chi, Bin Wang
Format: Article
Language:English
Published: Elsevier 2024-11-01
Series:Journal of Functional Foods
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Online Access:http://www.sciencedirect.com/science/article/pii/S1756464624005395
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author Xiao-Meng Dong
Shi-Kun Suo
Yu-Mei Wang
Yu-Hui Zeng
Chang-Feng Chi
Bin Wang
author_facet Xiao-Meng Dong
Shi-Kun Suo
Yu-Mei Wang
Yu-Hui Zeng
Chang-Feng Chi
Bin Wang
author_sort Xiao-Meng Dong
collection DOAJ
description In this study, we devoted attention to the hepatoprotective functions of high Fischer ratio oligopeptides (HFOPs) from Antarctic krill (HFOPs-AK) on alcoholic liver injury of mice. The results indicated that HFOPs-AK significantly improved the pathological state of mice liver and kidney, reduced alanine aminotransferase (ALT) and aspartate transaminase (AST) activities, regulated lipid metabolism, and alleviated the burden on the liver. In addition, HFOPs-AK depicted anti-inflammatory and antioxidant effects by reducing tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) contents and increasing superoxide dismutase (SOD) and catalase (CAT) activities to reduce malondialdehyde (MDA) level. The hepatoprotective mechanism of HFOPs-AK was further revealed by quantitative reverse transcription PCR (RT-qPCR) analysis, and the results proved that HFOPs-AK had the ability to stimulate the adenosine 5‘-monophosphate-activated protein kinase (AMPK)/nuclearrespiratoty factor 2 (Nrf2)/inhibitor of NF-κB (IκBα) signaling pathways, thereby regulating the expression levels of downstream factors. The activation of these signaling pathways is essential for the regulation of lipid metabolism as well as anti-inflammatory and antioxidant functions. This finding offers a novel approach for the development of innovative hepatoprotective agents utilizing HFOPs-AK.
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institution Kabale University
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publishDate 2024-11-01
publisher Elsevier
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series Journal of Functional Foods
spelling doaj-art-fd58a89c93f44e5da0fe0ac312d8d0e72024-11-16T05:10:51ZengElsevierJournal of Functional Foods1756-46462024-11-01122106537High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathwaysXiao-Meng Dong0Shi-Kun Suo1Yu-Mei Wang2Yu-Hui Zeng3Chang-Feng Chi4Bin Wang5Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, ChinaZhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, ChinaNational and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic Resources, National Engineering Research Center of Marine Facilities Aquaculture, School of Marine Science and Technology, Zhejiang Ocean University, Zhoushan 316022, China; Corresponding authors.Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical Products, School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, China; Corresponding authors.In this study, we devoted attention to the hepatoprotective functions of high Fischer ratio oligopeptides (HFOPs) from Antarctic krill (HFOPs-AK) on alcoholic liver injury of mice. The results indicated that HFOPs-AK significantly improved the pathological state of mice liver and kidney, reduced alanine aminotransferase (ALT) and aspartate transaminase (AST) activities, regulated lipid metabolism, and alleviated the burden on the liver. In addition, HFOPs-AK depicted anti-inflammatory and antioxidant effects by reducing tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6) contents and increasing superoxide dismutase (SOD) and catalase (CAT) activities to reduce malondialdehyde (MDA) level. The hepatoprotective mechanism of HFOPs-AK was further revealed by quantitative reverse transcription PCR (RT-qPCR) analysis, and the results proved that HFOPs-AK had the ability to stimulate the adenosine 5‘-monophosphate-activated protein kinase (AMPK)/nuclearrespiratoty factor 2 (Nrf2)/inhibitor of NF-κB (IκBα) signaling pathways, thereby regulating the expression levels of downstream factors. The activation of these signaling pathways is essential for the regulation of lipid metabolism as well as anti-inflammatory and antioxidant functions. This finding offers a novel approach for the development of innovative hepatoprotective agents utilizing HFOPs-AK.http://www.sciencedirect.com/science/article/pii/S1756464624005395Antarctic krill (Euphausia superba)High Fischer ratio oligopeptides (HFOPs)Alcoholic liver injuryLipid metabolismAntioxidant activityAnti-inflammatory activity
spellingShingle Xiao-Meng Dong
Shi-Kun Suo
Yu-Mei Wang
Yu-Hui Zeng
Chang-Feng Chi
Bin Wang
High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
Journal of Functional Foods
Antarctic krill (Euphausia superba)
High Fischer ratio oligopeptides (HFOPs)
Alcoholic liver injury
Lipid metabolism
Antioxidant activity
Anti-inflammatory activity
title High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
title_full High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
title_fullStr High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
title_full_unstemmed High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
title_short High Fischer ratio oligopeptides from Antarctic krill: Ameliorating function and mechanism to alcoholic liver injury through regulating AMPK/Nrf2/IκBα pathways
title_sort high fischer ratio oligopeptides from antarctic krill ameliorating function and mechanism to alcoholic liver injury through regulating ampk nrf2 iκbα pathways
topic Antarctic krill (Euphausia superba)
High Fischer ratio oligopeptides (HFOPs)
Alcoholic liver injury
Lipid metabolism
Antioxidant activity
Anti-inflammatory activity
url http://www.sciencedirect.com/science/article/pii/S1756464624005395
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