Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area
IntroductionIn ruminants, a symbiotic rumen microbiota is responsible for supporting the digestion of dietary fiber and contributes to health traits closely associated with meat and milk quality. A holistic view of the physicochemical profiles of mixed rumen microbiota (MRM) is not well-illustrated....
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1514952/full |
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| author | Yong Liu Junrui Liao Shaoxun Tang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Zhiliang Tan Abdelfattah Z. M. Salem |
| author_facet | Yong Liu Junrui Liao Shaoxun Tang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Zhiliang Tan Abdelfattah Z. M. Salem |
| author_sort | Yong Liu |
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| description | IntroductionIn ruminants, a symbiotic rumen microbiota is responsible for supporting the digestion of dietary fiber and contributes to health traits closely associated with meat and milk quality. A holistic view of the physicochemical profiles of mixed rumen microbiota (MRM) is not well-illustrated.MethodsThe experiment was performed with a 3 × 4 factorial arrangement of the specific surface area (SSA: 3.37, 3.73, and 4.44 m2/g) of NDF extracted from rice straw and the surface tension (ST: 54, 46, 43, and 36 dyn/cm) of a fermented medium in a fermentation time series of 6, 12, 24, 48 h with three experimental units. Here, we used three rumen-fistulated adult Liuyang black goats as the rumen liquid donors for this experiment.ResultsIt was found that increasing SSA decreased the average acetate/propionate ratio (A/P, p < 0.05) and increased the molarity of propionate (p < 0.05). Increasing ST decreased total volatile fatty acid (tVFA) concentration (p < 0.01). Greater SSA increased (p < 0.01) MRM hydrophobicity, whereas increasing ST increased MRM cell membrane permeability (p < 0.01). The neutral detergent fiber digestibility (NDFD, r = 0.937) and tVFA (r = 0.809) were positively correlated with the membrane permeability of MRM.DiscussionThe surface tension of the artificial medium and substrate-specific surface area had a significant influence on MRM's fermentation profiles, hydrophobicity, and permeability. The results suggest that physical environmental properties are key in regulating rumen fermentation function and homeostasis in the gastrointestinal tract ecosystem. |
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| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Frontiers in Veterinary Science |
| spelling | doaj-art-fd272d11dacd461abc1b47656c72067b2025-01-06T06:59:49ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15149521514952Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface areaYong Liu0Junrui Liao1Shaoxun Tang2Shaoxun Tang3Chuanshe Zhou4Chuanshe Zhou5Zhiliang Tan6Abdelfattah Z. M. Salem7CAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Science, Beijing, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan, ChinaCollege of Advanced Agricultural Sciences, University of Chinese Academy of Science, Beijing, ChinaCAS Key Laboratory for Agro-Ecological Processes in Subtropical Region, National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production, Hunan Provincial Key Laboratory of Animal Nutrition and Physiology and Metabolism, Institute of Subtropical Agriculture, the Chinese Academy of Sciences, Changsha, Hunan, ChinaFacultad de Medicina Veterinaria y Zootecnia, Universidad Autónoma del Estado de México, Toluca, MexicoIntroductionIn ruminants, a symbiotic rumen microbiota is responsible for supporting the digestion of dietary fiber and contributes to health traits closely associated with meat and milk quality. A holistic view of the physicochemical profiles of mixed rumen microbiota (MRM) is not well-illustrated.MethodsThe experiment was performed with a 3 × 4 factorial arrangement of the specific surface area (SSA: 3.37, 3.73, and 4.44 m2/g) of NDF extracted from rice straw and the surface tension (ST: 54, 46, 43, and 36 dyn/cm) of a fermented medium in a fermentation time series of 6, 12, 24, 48 h with three experimental units. Here, we used three rumen-fistulated adult Liuyang black goats as the rumen liquid donors for this experiment.ResultsIt was found that increasing SSA decreased the average acetate/propionate ratio (A/P, p < 0.05) and increased the molarity of propionate (p < 0.05). Increasing ST decreased total volatile fatty acid (tVFA) concentration (p < 0.01). Greater SSA increased (p < 0.01) MRM hydrophobicity, whereas increasing ST increased MRM cell membrane permeability (p < 0.01). The neutral detergent fiber digestibility (NDFD, r = 0.937) and tVFA (r = 0.809) were positively correlated with the membrane permeability of MRM.DiscussionThe surface tension of the artificial medium and substrate-specific surface area had a significant influence on MRM's fermentation profiles, hydrophobicity, and permeability. The results suggest that physical environmental properties are key in regulating rumen fermentation function and homeostasis in the gastrointestinal tract ecosystem.https://www.frontiersin.org/articles/10.3389/fvets.2024.1514952/fullphysicochemical propertiesrumen microbesspecific surface areasurface tensionphysical properties |
| spellingShingle | Yong Liu Junrui Liao Shaoxun Tang Shaoxun Tang Chuanshe Zhou Chuanshe Zhou Zhiliang Tan Abdelfattah Z. M. Salem Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area Frontiers in Veterinary Science physicochemical properties rumen microbes specific surface area surface tension physical properties |
| title | Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| title_full | Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| title_fullStr | Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| title_full_unstemmed | Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| title_short | Physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| title_sort | physicochemical profiles of mixed ruminal microbes in response to surface tension and specific surface area |
| topic | physicochemical properties rumen microbes specific surface area surface tension physical properties |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1514952/full |
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