Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet
A healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study eva...
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| Format: | Article |
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Frontiers Media S.A.
2025-08-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2025.1621709/full |
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| author | Chinmayee Panda Ralph Kruse Kaya Williams Brea Nance Maruti Gudavalli Shirin Pourafshar Brandon Metzger Slavko Komarnytsky Slavko Komarnytsky |
| author_facet | Chinmayee Panda Ralph Kruse Kaya Williams Brea Nance Maruti Gudavalli Shirin Pourafshar Brandon Metzger Slavko Komarnytsky Slavko Komarnytsky |
| author_sort | Chinmayee Panda |
| collection | DOAJ |
| description | A healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study evaluated a 21-day purification program emphasizing plant foods and whole food supplementation on metabolic detoxification, oxidative stress, and gut microbiota composition. Participants followed a plant-based diet for 7 days, with lean proteins introduced from days 8–21. Whole food supplements rich in polyphenols, glucosinolates, and prebiotic fibers were included to support metabolic and gastrointestinal function. The intervention group exhibited 9% (p < 0.0001) reduction in ROS-associated oxidative stress compared to diet alone, suggesting enhanced redox balance. Additionally, the intervention group exhibited higher urinary creatinine levels, indicating enhanced kidney filtration efficiency (p = 0.03). Self-reported stress levels decreased by 36% (p = 0.049) in the intervention group, with a notable reduction in anxiety related to future uncertainty, demonstrating the psychological benefits of dietary supplementation. Gut microbiome analysis revealed a reduction in Proteobacteria, a phylum associated with chronic metabolic and inflammatory diseases, and beneficial increases in Ruminococcus gnavus, Lactobacillus zeae, and Roseburia faecis, which contribute to gut barrier integrity and butyrate production. These findings highlight the potential of whole food-based supplementation to enhance dietary transitions by reducing oxidative stress, supporting kidney function, alleviating psychological stress, and modulating gut microbiota composition.Clinical trial registrationNCT05877365. |
| format | Article |
| id | doaj-art-fd0e2de4c8ca48a49c8c2f7e7754c0f5 |
| institution | Kabale University |
| issn | 2296-861X |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj-art-fd0e2de4c8ca48a49c8c2f7e7754c0f52025-08-20T03:40:28ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2025-08-011210.3389/fnut.2025.16217091621709Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier dietChinmayee Panda0Ralph Kruse1Kaya Williams2Brea Nance3Maruti Gudavalli4Shirin Pourafshar5Brandon Metzger6Slavko Komarnytsky7Slavko Komarnytsky8Nutrition Innovation Center, Standard Process Inc., Kannapolis, NC, United StatesKeiser University, College of Chiropractic Medicine, West Palm Beach, FL, United StatesNutrition Innovation Center, Standard Process Inc., Kannapolis, NC, United StatesNutrition Innovation Center, Standard Process Inc., Kannapolis, NC, United StatesKeiser University, College of Chiropractic Medicine, West Palm Beach, FL, United StatesNutrition Innovation Center, Standard Process Inc., Kannapolis, NC, United StatesNutrition Innovation Center, Standard Process Inc., Kannapolis, NC, United StatesPlants for Human Health Institute, NC State University, Kannapolis, NC, United StatesDepartment of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC, United StatesA healthy diet helps transition individuals towards numerous long-term metabolic benefits but is challenging to maintain. Supplementation with additional whole food-based concentrates and herbs may support this shift by enhancing metabolic resilience. This randomized, controlled, crossover study evaluated a 21-day purification program emphasizing plant foods and whole food supplementation on metabolic detoxification, oxidative stress, and gut microbiota composition. Participants followed a plant-based diet for 7 days, with lean proteins introduced from days 8–21. Whole food supplements rich in polyphenols, glucosinolates, and prebiotic fibers were included to support metabolic and gastrointestinal function. The intervention group exhibited 9% (p < 0.0001) reduction in ROS-associated oxidative stress compared to diet alone, suggesting enhanced redox balance. Additionally, the intervention group exhibited higher urinary creatinine levels, indicating enhanced kidney filtration efficiency (p = 0.03). Self-reported stress levels decreased by 36% (p = 0.049) in the intervention group, with a notable reduction in anxiety related to future uncertainty, demonstrating the psychological benefits of dietary supplementation. Gut microbiome analysis revealed a reduction in Proteobacteria, a phylum associated with chronic metabolic and inflammatory diseases, and beneficial increases in Ruminococcus gnavus, Lactobacillus zeae, and Roseburia faecis, which contribute to gut barrier integrity and butyrate production. These findings highlight the potential of whole food-based supplementation to enhance dietary transitions by reducing oxidative stress, supporting kidney function, alleviating psychological stress, and modulating gut microbiota composition.Clinical trial registrationNCT05877365.https://www.frontiersin.org/articles/10.3389/fnut.2025.1621709/fullwhole foodbotanicaldetoxificationoxidative stressgut microbiotametabolic health |
| spellingShingle | Chinmayee Panda Ralph Kruse Kaya Williams Brea Nance Maruti Gudavalli Shirin Pourafshar Brandon Metzger Slavko Komarnytsky Slavko Komarnytsky Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet Frontiers in Nutrition whole food botanical detoxification oxidative stress gut microbiota metabolic health |
| title | Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| title_full | Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| title_fullStr | Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| title_full_unstemmed | Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| title_short | Metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| title_sort | metabolic reset purification program improves antioxidant balance and gut microbiome in individuals transitioning to a healthier diet |
| topic | whole food botanical detoxification oxidative stress gut microbiota metabolic health |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2025.1621709/full |
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