Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain
Summary: The brain uses a specialized system to transport cerebrospinal fluid (CSF), consisting of interconnected ventricles lined by motile ciliated ependymal cells. These cells act jointly with CSF secretion and cardiac pressure gradients to regulate CSF dynamics. To date, the link between cilia-m...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Cell Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124724015468 |
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| author | Percival P. D’Gama Inyoung Jeong Andreas Moe Nygård Ahmed Jamali Emre Yaksi Nathalie Jurisch-Yaksi |
| author_facet | Percival P. D’Gama Inyoung Jeong Andreas Moe Nygård Ahmed Jamali Emre Yaksi Nathalie Jurisch-Yaksi |
| author_sort | Percival P. D’Gama |
| collection | DOAJ |
| description | Summary: The brain uses a specialized system to transport cerebrospinal fluid (CSF), consisting of interconnected ventricles lined by motile ciliated ependymal cells. These cells act jointly with CSF secretion and cardiac pressure gradients to regulate CSF dynamics. To date, the link between cilia-mediated CSF flow and brain function is poorly understood. Using zebrafish larvae as a model system, we identify that loss of ciliary motility does not alter progenitor proliferation, brain morphology, or spontaneous neural activity despite leading to an enlarged telencephalic ventricle. We observe altered neuronal responses to photic stimulations in the optic tectum and hindbrain and brain asymmetry defects in the habenula. Finally, we investigate astroglia since they contact CSF and regulate neuronal activity. Our analyses reveal a reduction in astroglial calcium signals during both spontaneous and light-evoked activity. Our findings highlight a role of motile cilia in regulating brain physiology through the modulation of neural and astroglial networks. |
| format | Article |
| id | doaj-art-fcfbc689736c4959bdbe0e898ac9cfe5 |
| institution | Kabale University |
| issn | 2211-1247 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Cell Reports |
| spelling | doaj-art-fcfbc689736c4959bdbe0e898ac9cfe52025-01-12T05:24:52ZengElsevierCell Reports2211-12472025-01-01441115195Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brainPercival P. D’Gama0Inyoung Jeong1Andreas Moe Nygård2Ahmed Jamali3Emre Yaksi4Nathalie Jurisch-Yaksi5Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgssons Gate 1, 7491 Trondheim, NorwayDepartment of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgssons Gate 1, 7491 Trondheim, NorwayDepartment of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgssons Gate 1, 7491 Trondheim, NorwayKavli Institute for Systems Neuroscience and Centre for Algorithms in the Cortex, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, NorwayKavli Institute for Systems Neuroscience and Centre for Algorithms in the Cortex, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway; Koç University Research Center for Translational Medicine, Koç University School of Medicine, Davutpaşa Caddesi, No:4, Topkapı, Istanbul 34010, TurkeyDepartment of Clinical and Molecular Medicine, Norwegian University of Science and Technology, Erling Skjalgssons Gate 1, 7491 Trondheim, Norway; Kavli Institute for Systems Neuroscience and Centre for Algorithms in the Cortex, Norwegian University of Science and Technology, Olav Kyrres Gate 9, 7030 Trondheim, Norway; Corresponding authorSummary: The brain uses a specialized system to transport cerebrospinal fluid (CSF), consisting of interconnected ventricles lined by motile ciliated ependymal cells. These cells act jointly with CSF secretion and cardiac pressure gradients to regulate CSF dynamics. To date, the link between cilia-mediated CSF flow and brain function is poorly understood. Using zebrafish larvae as a model system, we identify that loss of ciliary motility does not alter progenitor proliferation, brain morphology, or spontaneous neural activity despite leading to an enlarged telencephalic ventricle. We observe altered neuronal responses to photic stimulations in the optic tectum and hindbrain and brain asymmetry defects in the habenula. Finally, we investigate astroglia since they contact CSF and regulate neuronal activity. Our analyses reveal a reduction in astroglial calcium signals during both spontaneous and light-evoked activity. Our findings highlight a role of motile cilia in regulating brain physiology through the modulation of neural and astroglial networks.http://www.sciencedirect.com/science/article/pii/S2211124724015468CP: NeuroscienceCP: Developmental biology |
| spellingShingle | Percival P. D’Gama Inyoung Jeong Andreas Moe Nygård Ahmed Jamali Emre Yaksi Nathalie Jurisch-Yaksi Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain Cell Reports CP: Neuroscience CP: Developmental biology |
| title | Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| title_full | Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| title_fullStr | Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| title_full_unstemmed | Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| title_short | Motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| title_sort | motile cilia modulate neuronal and astroglial activity in the zebrafish larval brain |
| topic | CP: Neuroscience CP: Developmental biology |
| url | http://www.sciencedirect.com/science/article/pii/S2211124724015468 |
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