Study on the mechanism of MMP-9 inhibitor on biological behavior of human oralsquamous cell carcinoma cell line CAL27
[Objective:] To explore the potential of BB-94, a matrix metalloproteinase-9 (MMP-9) inhibitor, in alleviating malignant biological behavior of human oral squamous cell carcinoma (OSCC) cell line CAL27 and its possible mechanism. [Methods:] CAL27 cells in logarithmic growth phase were cultured in BB...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Oral and Maxillofacial Surgery
2023-12-01
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| Series: | Kouqiang hemian waike zazhi |
| Subjects: | |
| Online Access: | https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2023.06.002 |
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| Summary: | [Objective:] To explore the potential of BB-94, a matrix metalloproteinase-9 (MMP-9) inhibitor, in alleviating malignant biological behavior of human oral squamous cell carcinoma (OSCC) cell line CAL27 and its possible mechanism. [Methods:] CAL27 cells in logarithmic growth phase were cultured in BB-94 culture medium with different concentrations (0, 0.125, 0.25, 0.5, 1.0 μg/mL) for 48 hours; the activity of MMP-9 in cell culture supernatant was analyzed by gelatin zymogram; the cell survival rate was detected by cell counting kit-8 (CCK-8) method; the apoptosis, migration, and invasion of CAL27 cells were detected by flow cytometry, wound healing test and Transwell test; real-time quantitative polymerase chain reaction (RT-qPCR) was used to detect the mRNA expression levels of phosphatidylinositol 3-kinase (PI3K) and protein kinase B (Akt); the expressions of PI3K, phosphorylated protein kinase B (p-Akt), MMP-9, vimentin (Vim), N-cadherin (N-cad) and E-cadherin (E-cad) proteins were detected by Western blotting. [Results:] The relative activity of MMP-9, the survival rate of CAL27 cells, the wound healing rate, the number of cell invasion, the relative expression level of PI3K and Akt mRNA and the relative expression level of PI3K, p-Akt, Vim, N-cad and MMP-9 protein decreased with the increase in the concentration of BB-94 (P<0.05). The apoptosis rate of CAL27 cells and the relative expression level of E-cad protein increased as the concentration of BB-94 went up (P<0.05). [Conclusion:] These results indicate that MMP-9 inhibitor could inhibit the malignant biological behavior of human OSCC cell line CAL27, and its mechanism may be related to preventing the activation of PI3K/Akt signaling pathway and the progress of epithelial mesenchymal transition (EMT). |
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| ISSN: | 1005-4979 |