Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils
Scanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their...
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MDPI AG
2024-10-01
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| Series: | Cells |
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| author | Mikhail Shvedov Ekaterina Sherstyukova Snezhanna Kandrashina Vladimir Inozemtsev Viktoria Sergunova |
| author_facet | Mikhail Shvedov Ekaterina Sherstyukova Snezhanna Kandrashina Vladimir Inozemtsev Viktoria Sergunova |
| author_sort | Mikhail Shvedov |
| collection | DOAJ |
| description | Scanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their structural and functional characteristics at the nanoscale. Its long-standing application in this field highlights its essential role in cell biology and immunology research, significantly contributing to understanding cellular mechanics and interactions. In this review, we discuss the application of SPM techniques, specifically atomic force microscopy (AFM) and scanning ion-conductance microscopy (SICM), to study the fundamental functions of neutrophils. In addition, recent advances in the application of SPM in single-cell immunology are discussed. The application of these techniques allows for obtaining data on the morphology, topography, and mechanical and electrochemical properties of neutrophils with high accuracy. |
| format | Article |
| id | doaj-art-fcc8c597ee5c4532bdd41382f9403fa9 |
| institution | Kabale University |
| issn | 2073-4409 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj-art-fcc8c597ee5c4532bdd41382f9403fa92024-11-08T14:34:24ZengMDPI AGCells2073-44092024-10-011321175710.3390/cells13211757Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of NeutrophilsMikhail Shvedov0Ekaterina Sherstyukova1Snezhanna Kandrashina2Vladimir Inozemtsev3Viktoria Sergunova4Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaFederal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaScanning probe microscopy (SPM) is a versatile tool for studying a wide range of materials. It is well suited for investigating living matter, for example, in single-cell neutrophil studies. SPM has been extensively utilized to analyze cell physical properties, providing detailed insights into their structural and functional characteristics at the nanoscale. Its long-standing application in this field highlights its essential role in cell biology and immunology research, significantly contributing to understanding cellular mechanics and interactions. In this review, we discuss the application of SPM techniques, specifically atomic force microscopy (AFM) and scanning ion-conductance microscopy (SICM), to study the fundamental functions of neutrophils. In addition, recent advances in the application of SPM in single-cell immunology are discussed. The application of these techniques allows for obtaining data on the morphology, topography, and mechanical and electrochemical properties of neutrophils with high accuracy.https://www.mdpi.com/2073-4409/13/21/1757AFMSICMneutrophilsNETosiselastic modulusROS |
| spellingShingle | Mikhail Shvedov Ekaterina Sherstyukova Snezhanna Kandrashina Vladimir Inozemtsev Viktoria Sergunova Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils Cells AFM SICM neutrophils NETosis elastic modulus ROS |
| title | Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils |
| title_full | Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils |
| title_fullStr | Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils |
| title_full_unstemmed | Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils |
| title_short | Atomic Force Microscopy and Scanning Ion-Conductance Microscopy for Investigation of Biomechanical Characteristics of Neutrophils |
| title_sort | atomic force microscopy and scanning ion conductance microscopy for investigation of biomechanical characteristics of neutrophils |
| topic | AFM SICM neutrophils NETosis elastic modulus ROS |
| url | https://www.mdpi.com/2073-4409/13/21/1757 |
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