Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis
ObjectiveIn clinical practice, an accurate and efficient detection approach for pulmonary tuberculosis (PTB) is highly needed. The fluorescence in situ hybridization (FISH) assay for PTB might be a suitable alternative to current tests. However, a systematic assessment of the diagnostic performance...
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Frontiers Media S.A.
2025-01-01
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2024.1467530/full |
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| author | Yu-Qi Hu Yu-Qi Hu Kang Liu Kang Liu Le-Qing Lai Le-Qing Lai Yi-Ru He Yi-Ru He Li-Ping Hong Li-Ping Hong Chu-Qian Jiang Chu-Qian Jiang Si-Min Liu Si-Min Liu Ming-Zhu Cao |
| author_facet | Yu-Qi Hu Yu-Qi Hu Kang Liu Kang Liu Le-Qing Lai Le-Qing Lai Yi-Ru He Yi-Ru He Li-Ping Hong Li-Ping Hong Chu-Qian Jiang Chu-Qian Jiang Si-Min Liu Si-Min Liu Ming-Zhu Cao |
| author_sort | Yu-Qi Hu |
| collection | DOAJ |
| description | ObjectiveIn clinical practice, an accurate and efficient detection approach for pulmonary tuberculosis (PTB) is highly needed. The fluorescence in situ hybridization (FISH) assay for PTB might be a suitable alternative to current tests. However, a systematic assessment of the diagnostic performance of this new approach is not available. Our study aimed to determine the diagnostic accuracy of FISH for PTB.MethodsWe examined PubMed and three more databases including Embase, Cochrane Library, and Web of Science databases from their establishment to November 10, 2023, for published articles on the diagnostic performance of FISH on individuals with clinical suspicion of tuberculosis (TB). QUADAS-2 was used to evaluate the literature’s quality. We used Meta-DiSc software to create forest plots.ResultsThe search yielded 7 studies, involving 1,224 sputum samples that could be included in our meta-analysis. The combined FISH sensitivity and specificity were 0.89 (95% CI 0.86–0.92) and 0.98 (95% CI 0.97–0.99), respectively. Furthermore, subgroup analysis was performed based on probes and PTB incidence.ConclusionFISH may be useful in the diagnosis of pulmonary tuberculosis. The sensitivity and specificity of FISH are high for most sputum specimens. Additionally, FISH has better diagnostic performance in countries with low PTB prevalence than in high PTB prevalence countries. We hope this study will find a new and effective tool for the early diagnosis of PTB. |
| format | Article |
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| institution | Kabale University |
| issn | 2296-858X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj-art-fcba27d8e3a945cd8e797755ec1ecc0f2025-01-06T04:13:02ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2025-01-011110.3389/fmed.2024.14675301467530Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysisYu-Qi Hu0Yu-Qi Hu1Kang Liu2Kang Liu3Le-Qing Lai4Le-Qing Lai5Yi-Ru He6Yi-Ru He7Li-Ping Hong8Li-Ping Hong9Chu-Qian Jiang10Chu-Qian Jiang11Si-Min Liu12Si-Min Liu13Ming-Zhu Cao14Department of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Second Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaDepartment of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou, ChinaDepartment of Obstetrics and Gynecology, Center for Reproductive Medicine, Guangdong Provincial Key Laboratory of Major Obstetric Diseases, Guangdong Provincial Clinical Research Center for Obstetrics and Gynecology, Guangdong-Hong Kong-Macao Greater Bay Area Higher Education Joint Laboratory of Maternal-Fetal Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou, ChinaObjectiveIn clinical practice, an accurate and efficient detection approach for pulmonary tuberculosis (PTB) is highly needed. The fluorescence in situ hybridization (FISH) assay for PTB might be a suitable alternative to current tests. However, a systematic assessment of the diagnostic performance of this new approach is not available. Our study aimed to determine the diagnostic accuracy of FISH for PTB.MethodsWe examined PubMed and three more databases including Embase, Cochrane Library, and Web of Science databases from their establishment to November 10, 2023, for published articles on the diagnostic performance of FISH on individuals with clinical suspicion of tuberculosis (TB). QUADAS-2 was used to evaluate the literature’s quality. We used Meta-DiSc software to create forest plots.ResultsThe search yielded 7 studies, involving 1,224 sputum samples that could be included in our meta-analysis. The combined FISH sensitivity and specificity were 0.89 (95% CI 0.86–0.92) and 0.98 (95% CI 0.97–0.99), respectively. Furthermore, subgroup analysis was performed based on probes and PTB incidence.ConclusionFISH may be useful in the diagnosis of pulmonary tuberculosis. The sensitivity and specificity of FISH are high for most sputum specimens. Additionally, FISH has better diagnostic performance in countries with low PTB prevalence than in high PTB prevalence countries. We hope this study will find a new and effective tool for the early diagnosis of PTB.https://www.frontiersin.org/articles/10.3389/fmed.2024.1467530/fullfluorescence in situ hybridization (FISH)pulmonary tuberculosisdiagnostic accuracymeta-analysisprobes (sensors) |
| spellingShingle | Yu-Qi Hu Yu-Qi Hu Kang Liu Kang Liu Le-Qing Lai Le-Qing Lai Yi-Ru He Yi-Ru He Li-Ping Hong Li-Ping Hong Chu-Qian Jiang Chu-Qian Jiang Si-Min Liu Si-Min Liu Ming-Zhu Cao Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis Frontiers in Medicine fluorescence in situ hybridization (FISH) pulmonary tuberculosis diagnostic accuracy meta-analysis probes (sensors) |
| title | Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis |
| title_full | Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis |
| title_fullStr | Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis |
| title_full_unstemmed | Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis |
| title_short | Evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis: a systematic review and meta-analysis |
| title_sort | evaluation of the diagnostic efficiency of fluorescence in situ hybridization for pulmonary tuberculosis a systematic review and meta analysis |
| topic | fluorescence in situ hybridization (FISH) pulmonary tuberculosis diagnostic accuracy meta-analysis probes (sensors) |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2024.1467530/full |
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