Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration
Citri Reticulatae Pericarpium ‘Chachi’ (Citrus reticulata ‘Chachi’) is a traditional Chinese medicine with dual medicinal and dietary uses. As its main component, pectin exhibited various biological activities. However, research and development on it, especially pectin oligosaccharides, remain limit...
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Elsevier
2024-12-01
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| Series: | Food Hydrocolloids for Health |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667025924000104 |
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| author | Zhongcan Peng Shurong Tian Depo Yang Longping Zhu Jianing Zhang Wenfeng Li Guodong Zheng Zhimin Zhao |
| author_facet | Zhongcan Peng Shurong Tian Depo Yang Longping Zhu Jianing Zhang Wenfeng Li Guodong Zheng Zhimin Zhao |
| author_sort | Zhongcan Peng |
| collection | DOAJ |
| description | Citri Reticulatae Pericarpium ‘Chachi’ (Citrus reticulata ‘Chachi’) is a traditional Chinese medicine with dual medicinal and dietary uses. As its main component, pectin exhibited various biological activities. However, research and development on it, especially pectin oligosaccharides, remain limited. In this study, ‘Chachi’ pectin oligosaccharides (CPOS) was extracted and prepared for the first time, resulting in the purification of two oligosaccharide components, CPOS3 and CPOS4. Structural characterization of the predominant oligosaccharide, CPOS3, revealed it to be composed of a backbone α-D-GalpA-(1→2)-α-L-Rhap-(1→4)-α-D-3-OAc-GalpA-(1→4)-α-D-GalpA-(1→4)-α-D-GalpA and a side chain [→5)-α-L-Araf-(1→]4 attached at the C-4 of Rhap. Subsequent experiments have shown that CPOS3 can significantly promote the proliferation and migration of human immortalized keratinocyte cell. Further investigation revealed that it facilitated cell proliferation by stimulating DNA synthesis in the S phase of the cell cycle. Additionally, CPOS3 exhibited good thermal stability and rheological characteristics. Based on the good wound healing activity and favorable physicochemical properties of CPOS3, its prospects in the fields of wound dressings and tissue repair are promising. |
| format | Article |
| id | doaj-art-fc80e4f0e94e446f8873255940b20cf0 |
| institution | Kabale University |
| issn | 2667-0259 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Hydrocolloids for Health |
| spelling | doaj-art-fc80e4f0e94e446f8873255940b20cf02024-12-17T05:01:39ZengElsevierFood Hydrocolloids for Health2667-02592024-12-016100185Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migrationZhongcan Peng0Shurong Tian1Depo Yang2Longping Zhu3Jianing Zhang4Wenfeng Li5Guodong Zheng6Zhimin Zhao7School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, ChinaSchool of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou, 511436, China; Corresponding author.School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China; Guangdong Technology Research Center for Advanced Chinese Medicine, Guangzhou 510006, China; Corresponding author at: School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, 510006, China.Citri Reticulatae Pericarpium ‘Chachi’ (Citrus reticulata ‘Chachi’) is a traditional Chinese medicine with dual medicinal and dietary uses. As its main component, pectin exhibited various biological activities. However, research and development on it, especially pectin oligosaccharides, remain limited. In this study, ‘Chachi’ pectin oligosaccharides (CPOS) was extracted and prepared for the first time, resulting in the purification of two oligosaccharide components, CPOS3 and CPOS4. Structural characterization of the predominant oligosaccharide, CPOS3, revealed it to be composed of a backbone α-D-GalpA-(1→2)-α-L-Rhap-(1→4)-α-D-3-OAc-GalpA-(1→4)-α-D-GalpA-(1→4)-α-D-GalpA and a side chain [→5)-α-L-Araf-(1→]4 attached at the C-4 of Rhap. Subsequent experiments have shown that CPOS3 can significantly promote the proliferation and migration of human immortalized keratinocyte cell. Further investigation revealed that it facilitated cell proliferation by stimulating DNA synthesis in the S phase of the cell cycle. Additionally, CPOS3 exhibited good thermal stability and rheological characteristics. Based on the good wound healing activity and favorable physicochemical properties of CPOS3, its prospects in the fields of wound dressings and tissue repair are promising.http://www.sciencedirect.com/science/article/pii/S2667025924000104Citrus reticulata ‘Chachi’Pectin oligosaccharideStructural analysisWound healing |
| spellingShingle | Zhongcan Peng Shurong Tian Depo Yang Longping Zhu Jianing Zhang Wenfeng Li Guodong Zheng Zhimin Zhao Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration Food Hydrocolloids for Health Citrus reticulata ‘Chachi’ Pectin oligosaccharide Structural analysis Wound healing |
| title | Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration |
| title_full | Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration |
| title_fullStr | Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration |
| title_full_unstemmed | Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration |
| title_short | Pectin oligosaccharides from Citri Reticulatae Pericarpium ‘Chachi’ promote wound healing in HaCaT keratinocytes by enhancing cell proliferation and migration |
| title_sort | pectin oligosaccharides from citri reticulatae pericarpium chachi promote wound healing in hacat keratinocytes by enhancing cell proliferation and migration |
| topic | Citrus reticulata ‘Chachi’ Pectin oligosaccharide Structural analysis Wound healing |
| url | http://www.sciencedirect.com/science/article/pii/S2667025924000104 |
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