Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis
Lagerstroemia speciosa is traditionally used for treating diabetes and inflammation; however, its anticancer potential remains unexplored. This study assesses the antioxidant and anticancer activities of L. speciosa bark extract, with a focus on Ehrlich Ascites Carcinoma (EAC) cells in mice. The cru...
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Elsevier
2025-09-01
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| Series: | Journal of Genetic Engineering and Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1687157X25000976 |
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| author | Shahnaz Parvin Sweety Tahsin Ahmed Rupok Mst. Shahnaj Parvin Jaytirmoy Barmon Mst. Sarmina Yeasmin Md. Ekramul Islam |
| author_facet | Shahnaz Parvin Sweety Tahsin Ahmed Rupok Mst. Shahnaj Parvin Jaytirmoy Barmon Mst. Sarmina Yeasmin Md. Ekramul Islam |
| author_sort | Shahnaz Parvin Sweety |
| collection | DOAJ |
| description | Lagerstroemia speciosa is traditionally used for treating diabetes and inflammation; however, its anticancer potential remains unexplored. This study assesses the antioxidant and anticancer activities of L. speciosa bark extract, with a focus on Ehrlich Ascites Carcinoma (EAC) cells in mice. The crude methanol extract (CME) was subdivided into n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), and aqueous (AQF) fractions. In vitro antioxidant assays identified CHF as the most active fraction, exhibiting high phenolic and flavonoid content. CHF and EAF were further investigated for anticancer activity in an EAC-induced mouse model, where CHF significantly (P < 0.05) reduced tumor cell count compared to EAF. Phytochemical characterization using FTIR and GC–MS revealed bioactive compounds, including 9-Methoxybicyclo[6.1.0]nona-2,4,6-triene and 9,12-Octadecadienoic acid methyl ester (E,E). Molecular docking studies demonstrated strong interactions between these compounds and key cancer-related proteins, p53 and Topoisomerase-II, suggesting potential anticancer mechanisms. Overall, this study shows that L. speciosa bark extract has therapeutic potential, especially for CHF. Bioactive compounds like 9,12-octadecadienoic acid methyl ester (E,E) and 9-methoxybicyclo [6.1.0]nona-2,4,6-triene contribute to the extract’s antioxidant and anticancer effects in EAC models, possibly by modulating p53 and Topoisomerase-II. |
| format | Article |
| id | doaj-art-fc67d2e195b142dca46ff2532c9482eb |
| institution | Kabale University |
| issn | 1687-157X |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Genetic Engineering and Biotechnology |
| spelling | doaj-art-fc67d2e195b142dca46ff2532c9482eb2025-08-24T05:11:56ZengElsevierJournal of Genetic Engineering and Biotechnology1687-157X2025-09-0123310055310.1016/j.jgeb.2025.100553Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysisShahnaz Parvin Sweety0Tahsin Ahmed Rupok1Mst. Shahnaj Parvin2Jaytirmoy Barmon3Mst. Sarmina Yeasmin4Md. Ekramul Islam5Department of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh; Department of Pharmacy, Manarat International University, Dhaka 1212, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, BangladeshBangladesh Council of Scientific and Industrial Research (BCSIR), Rajshahi Laboratories, Rajshahi 6206, BangladeshBangladesh Council of Scientific and Industrial Research (BCSIR), Rajshahi Laboratories, Rajshahi 6206, BangladeshDepartment of Pharmacy, University of Rajshahi, Rajshahi 6205, Bangladesh; Corresponding author at: Department of Pharmacy, Faculty of Science, University of Rajshahi, Rajshahi 6205, Bangladesh.Lagerstroemia speciosa is traditionally used for treating diabetes and inflammation; however, its anticancer potential remains unexplored. This study assesses the antioxidant and anticancer activities of L. speciosa bark extract, with a focus on Ehrlich Ascites Carcinoma (EAC) cells in mice. The crude methanol extract (CME) was subdivided into n-hexane (NHF), chloroform (CHF), ethyl acetate (EAF), and aqueous (AQF) fractions. In vitro antioxidant assays identified CHF as the most active fraction, exhibiting high phenolic and flavonoid content. CHF and EAF were further investigated for anticancer activity in an EAC-induced mouse model, where CHF significantly (P < 0.05) reduced tumor cell count compared to EAF. Phytochemical characterization using FTIR and GC–MS revealed bioactive compounds, including 9-Methoxybicyclo[6.1.0]nona-2,4,6-triene and 9,12-Octadecadienoic acid methyl ester (E,E). Molecular docking studies demonstrated strong interactions between these compounds and key cancer-related proteins, p53 and Topoisomerase-II, suggesting potential anticancer mechanisms. Overall, this study shows that L. speciosa bark extract has therapeutic potential, especially for CHF. Bioactive compounds like 9,12-octadecadienoic acid methyl ester (E,E) and 9-methoxybicyclo [6.1.0]nona-2,4,6-triene contribute to the extract’s antioxidant and anticancer effects in EAC models, possibly by modulating p53 and Topoisomerase-II.http://www.sciencedirect.com/science/article/pii/S1687157X25000976Lagerstroemia speciosaAntioxidantAnticancerFTIRGC–MSIn silico molecular docking |
| spellingShingle | Shahnaz Parvin Sweety Tahsin Ahmed Rupok Mst. Shahnaj Parvin Jaytirmoy Barmon Mst. Sarmina Yeasmin Md. Ekramul Islam Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis Journal of Genetic Engineering and Biotechnology Lagerstroemia speciosa Antioxidant Anticancer FTIR GC–MS In silico molecular docking |
| title | Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| title_full | Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| title_fullStr | Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| title_full_unstemmed | Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| title_short | Exploring the anticancer and antioxidant properties of Lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| title_sort | exploring the anticancer and antioxidant properties of lagerstroemia speciosa bark extract via phytochemical and molecular docking analysis |
| topic | Lagerstroemia speciosa Antioxidant Anticancer FTIR GC–MS In silico molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S1687157X25000976 |
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