Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle
Cattle coat color is governed by numerous genes, notably the tyrosinase gene (TYR). This study analyzed coat color anomalies like albinism and white spotting in Bali cattle. It aims to discern the TYR gene's diversity, expression patterns, and correlation with coat color abnormalities. The rese...
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| Format: | Article |
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Diponegoro University, Faculty of Animal and Agricultural Sciences
2024-12-01
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| Series: | Journal of the Indonesian Tropical Animal Agriculture |
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| Online Access: | https://ejournal.undip.ac.id/index.php/jitaa/article/view/59579 |
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| author | K. Kholijah S. Darwati M. F. Ulum I. M. Londra R. R. Noor J. Jakaria |
| author_facet | K. Kholijah S. Darwati M. F. Ulum I. M. Londra R. R. Noor J. Jakaria |
| author_sort | K. Kholijah |
| collection | DOAJ |
| description | Cattle coat color is governed by numerous genes, notably the tyrosinase gene (TYR). This study analyzed coat color anomalies like albinism and white spotting in Bali cattle. It aims to discern the TYR gene's diversity, expression patterns, and correlation with coat color abnormalities. The research encompassed 189 cattle, including those with standard coat color (n=53), white-spotted (n=11), and albino (n=17) Bali cattle, as well as Simmental (n=37), Limousin (n=14), Madura (n=21), and Peran-akan Ongole (PO) cattle (n=36). Total DNA was extracted and the TYR gene in exon 1 was amplified using forward and reverse primers with a target amplicon length of 994 bp. Direct sequencing unveiled TYR gene diversity, analyzed using BioEdit and MEGA6 software to identify SNPs. PCR-RFLP was used for SNP genotyping, while qPCR analyzed TYR gene expression. Two mutations (SNP g. 939A>G and SNP g. 887C>T) were discovered in Bali cattle TYR exon 1. SNP g. 939A>G exhibited polymorphism, with the highest GG genotype frequency in standard Bali cattle, indicating a high G allele frequency. Conversely, Madura, Simmental, Limousin, and PO cattle had the lowest allele fre-quency. Chi-square (χ² test) results showed non-significance across all cattle types. TYR gene expres-sion differed significantly between standard Bali cattle and albinos (p<0.05). |
| format | Article |
| id | doaj-art-fc5bad7b09fd48709fbe49363b5d395e |
| institution | Kabale University |
| issn | 2087-8273 2460-6278 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Diponegoro University, Faculty of Animal and Agricultural Sciences |
| record_format | Article |
| series | Journal of the Indonesian Tropical Animal Agriculture |
| spelling | doaj-art-fc5bad7b09fd48709fbe49363b5d395e2024-12-01T04:17:44ZengDiponegoro University, Faculty of Animal and Agricultural SciencesJournal of the Indonesian Tropical Animal Agriculture2087-82732460-62782024-12-0149429730610.14710/jitaa.49.4.297-30624851Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattleK. Kholijah0https://orcid.org/0009-0002-9161-9177S. Darwati1https://orcid.org/0000-0002-4882-3722M. F. Ulum2https://orcid.org/0000-0003-2092-9645I. M. Londra3https://orcid.org/0009-0001-4317-8402R. R. Noor4https://orcid.org/0000-0001-8795-2992J. Jakaria5https://orcid.org/0000-0001-7921-8528Graduate School of Animal Production and Technology, Faculty of Animal Science, IPB University (Bogor Agricultural University), IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, IPB University (Bogor Agricultural University), IndonesiaDivision of Reproduction and Obstetrics, School of Veterinary Medicine and Biomedical Sciences, IPB University (Bogor Agricultural University), IndonesiaAgricultural Technology Study Center (BPTP), IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, IPB University (Bogor Agricultural University), IndonesiaDepartment of Animal Production and Technology, Faculty of Animal Science, IPB University (Bogor Agricultural University), IndonesiaCattle coat color is governed by numerous genes, notably the tyrosinase gene (TYR). This study analyzed coat color anomalies like albinism and white spotting in Bali cattle. It aims to discern the TYR gene's diversity, expression patterns, and correlation with coat color abnormalities. The research encompassed 189 cattle, including those with standard coat color (n=53), white-spotted (n=11), and albino (n=17) Bali cattle, as well as Simmental (n=37), Limousin (n=14), Madura (n=21), and Peran-akan Ongole (PO) cattle (n=36). Total DNA was extracted and the TYR gene in exon 1 was amplified using forward and reverse primers with a target amplicon length of 994 bp. Direct sequencing unveiled TYR gene diversity, analyzed using BioEdit and MEGA6 software to identify SNPs. PCR-RFLP was used for SNP genotyping, while qPCR analyzed TYR gene expression. Two mutations (SNP g. 939A>G and SNP g. 887C>T) were discovered in Bali cattle TYR exon 1. SNP g. 939A>G exhibited polymorphism, with the highest GG genotype frequency in standard Bali cattle, indicating a high G allele frequency. Conversely, Madura, Simmental, Limousin, and PO cattle had the lowest allele fre-quency. Chi-square (χ² test) results showed non-significance across all cattle types. TYR gene expres-sion differed significantly between standard Bali cattle and albinos (p<0.05).https://ejournal.undip.ac.id/index.php/jitaa/article/view/59579bali cattlecoat colorpcr-rflpsnptyr gene expression |
| spellingShingle | K. Kholijah S. Darwati M. F. Ulum I. M. Londra R. R. Noor J. Jakaria Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle Journal of the Indonesian Tropical Animal Agriculture bali cattle coat color pcr-rflp snp tyr gene expression |
| title | Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle |
| title_full | Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle |
| title_fullStr | Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle |
| title_full_unstemmed | Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle |
| title_short | Investigation of polymorphism and expression of the tyrosinase (TYR) gene as a gene controlling coat color in Bali cattle |
| title_sort | investigation of polymorphism and expression of the tyrosinase tyr gene as a gene controlling coat color in bali cattle |
| topic | bali cattle coat color pcr-rflp snp tyr gene expression |
| url | https://ejournal.undip.ac.id/index.php/jitaa/article/view/59579 |
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