[N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer
The purpose of this study was to investigate the expression of glutamine synthetase (GS) in prostate cancer (PCa) and the utility of [ 13 N]ammonia positron emission tomography/computed tomography (PET/CT) in the imaging of PCa. The uptake ratio of [ 13 N]ammonia and the expression of GS in PC3 and...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2015-01-01
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| Series: | Molecular Imaging |
| Online Access: | https://doi.org/10.2310/7290.2014.00048 |
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| author | Xinchong Shi Xiangsong Zhang Chang Yi Yubo Liu Qiao He |
| author_facet | Xinchong Shi Xiangsong Zhang Chang Yi Yubo Liu Qiao He |
| author_sort | Xinchong Shi |
| collection | DOAJ |
| description | The purpose of this study was to investigate the expression of glutamine synthetase (GS) in prostate cancer (PCa) and the utility of [ 13 N]ammonia positron emission tomography/computed tomography (PET/CT) in the imaging of PCa. The uptake ratio of [ 13 N]ammonia and the expression of GS in PC3 and DU145 cells was measured. Thirty-four patients with suspected PCa underwent [ 13 N]ammonia PET/CT imaging, and immunohistochemistry staining of GS was performed. The uptake of [ 13 N]ammonia in PC3 and DU145 cells elevated along with the decrease in glutamine in medium. The expression of GS messenger ribonucleic acid and protein also increased when glutamine was deprived. In biopsy samples, the GS expression scores were significantly higher in PCa tissue than in benign tissues ( p < .001), and there was a positive correlation between the maximum GS expression scores and Gleason scores (Spearman r = .52). In 34 patients, [ 13 N]ammonia uptake in PCa segments was significantly higher than that in benign segments ( p ≤ .01), and there was a weak correlation between GS expression scores and the uptake of [ 13 N]ammonia (Spearman r = .47). The expression of GS in PCa cells upregulated along with the deprivation of glutamine. GS is the main reason for the uptake of [ 13 N]ammonia, and [ 13 N]ammonia is a useful tracer for PCa imaging. |
| format | Article |
| id | doaj-art-fc43651a0f5a4383962cd5bcf1ecb814 |
| institution | Kabale University |
| issn | 1536-0121 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Molecular Imaging |
| spelling | doaj-art-fc43651a0f5a4383962cd5bcf1ecb8142025-01-02T22:39:33ZengSAGE PublishingMolecular Imaging1536-01212015-01-011410.2310/7290.2014.0004810.2310_7290.2014.00048[N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate CancerXinchong ShiXiangsong ZhangChang YiYubo LiuQiao HeThe purpose of this study was to investigate the expression of glutamine synthetase (GS) in prostate cancer (PCa) and the utility of [ 13 N]ammonia positron emission tomography/computed tomography (PET/CT) in the imaging of PCa. The uptake ratio of [ 13 N]ammonia and the expression of GS in PC3 and DU145 cells was measured. Thirty-four patients with suspected PCa underwent [ 13 N]ammonia PET/CT imaging, and immunohistochemistry staining of GS was performed. The uptake of [ 13 N]ammonia in PC3 and DU145 cells elevated along with the decrease in glutamine in medium. The expression of GS messenger ribonucleic acid and protein also increased when glutamine was deprived. In biopsy samples, the GS expression scores were significantly higher in PCa tissue than in benign tissues ( p < .001), and there was a positive correlation between the maximum GS expression scores and Gleason scores (Spearman r = .52). In 34 patients, [ 13 N]ammonia uptake in PCa segments was significantly higher than that in benign segments ( p ≤ .01), and there was a weak correlation between GS expression scores and the uptake of [ 13 N]ammonia (Spearman r = .47). The expression of GS in PCa cells upregulated along with the deprivation of glutamine. GS is the main reason for the uptake of [ 13 N]ammonia, and [ 13 N]ammonia is a useful tracer for PCa imaging.https://doi.org/10.2310/7290.2014.00048 |
| spellingShingle | Xinchong Shi Xiangsong Zhang Chang Yi Yubo Liu Qiao He [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer Molecular Imaging |
| title | [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer |
| title_full | [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer |
| title_fullStr | [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer |
| title_full_unstemmed | [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer |
| title_short | [N]Ammonia Positron Emission Tomographic/Computed Tomographic Imaging Targeting Glutamine Synthetase Expression in Prostate Cancer |
| title_sort | n ammonia positron emission tomographic computed tomographic imaging targeting glutamine synthetase expression in prostate cancer |
| url | https://doi.org/10.2310/7290.2014.00048 |
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