The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer
IntroductionPrimary cilia play an important role in the development of cancer by regulating signaling pathways. Several studies have demonstrated that women with BRCA mutations have, on average, 50% fewer ciliated cells compared with general women. However, the role of tubal cilia loss in the develo...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2024.1495753/full |
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| author | Yuanli Guo Yuanli Guo Xinxin He Junfeng Liu Yanming Tan Chao Zhang Shan Chen Shan Chen Sheng Zhang |
| author_facet | Yuanli Guo Yuanli Guo Xinxin He Junfeng Liu Yanming Tan Chao Zhang Shan Chen Shan Chen Sheng Zhang |
| author_sort | Yuanli Guo |
| collection | DOAJ |
| description | IntroductionPrimary cilia play an important role in the development of cancer by regulating signaling pathways. Several studies have demonstrated that women with BRCA mutations have, on average, 50% fewer ciliated cells compared with general women. However, the role of tubal cilia loss in the development of epithelial ovarian cancer (EOC) remains unclear. Few specific studies have been found in linking HYDIN, a ciliary defect associated gene that encodes HYDIN axonemal central pair apparatus protein, which is involved in the transduction of Hedgehog (Hh) signal and is considered a cancer associated antigen, to ovarian cancer. Therefore, our study aimed to investigate the correlation between HYDIN gene mutations and tubal cilia loss in EOC.MethodsA whole exome sequencing (WES), immunohistochemistry (IHC), western blot, and reverse transcription quantitative (RT q) PCR were performed in 80 patients with EOC and 50 cases of non ovarian cancer to detect the mutations and expression of tubal ciliary marker, ciliary morphology, and abnormal rate.ResultsWe found that the incidence of tubal cilia loss was higher in EOC group with decreased expression of HYDIN compared with the control group (P<0.05).DiscussionThis study suggests that tubal ciliary loss is evident in epithelial fallopian tube carcinoma, and ciliary cells may be involved in the occurrence and development of EOC, and cilia-related gene HYDIN is expected to be a tumor marker for epithelial ovarian cancer. |
| format | Article |
| id | doaj-art-fc36090a184a477c9cfbf9acf04f541d |
| institution | Kabale University |
| issn | 2234-943X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Oncology |
| spelling | doaj-art-fc36090a184a477c9cfbf9acf04f541d2025-01-09T06:10:29ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2025-01-011410.3389/fonc.2024.14957531495753The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancerYuanli Guo0Yuanli Guo1Xinxin He2Junfeng Liu3Yanming Tan4Chao Zhang5Shan Chen6Shan Chen7Sheng Zhang8Department of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaDepartment of Obstetrics and Gynecology, The First Affiliated Hospital of Guangdong Pharmaceutical University, Guangzhou, ChinaDepartment of Pathology, Sun Yat-sen Memorial Hospital of Sun Yat-sen University, Guangzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou, ChinaGenePlus-Shenzhen, Shenzhen, ChinaGenePlus-Shenzhen, Shenzhen, ChinaDepartment of Gynecology, The Six Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaBiomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, ChinaDepartment of Pathology, The First Affiliated Hospital of Fujian Medical University, Fuzhou, ChinaIntroductionPrimary cilia play an important role in the development of cancer by regulating signaling pathways. Several studies have demonstrated that women with BRCA mutations have, on average, 50% fewer ciliated cells compared with general women. However, the role of tubal cilia loss in the development of epithelial ovarian cancer (EOC) remains unclear. Few specific studies have been found in linking HYDIN, a ciliary defect associated gene that encodes HYDIN axonemal central pair apparatus protein, which is involved in the transduction of Hedgehog (Hh) signal and is considered a cancer associated antigen, to ovarian cancer. Therefore, our study aimed to investigate the correlation between HYDIN gene mutations and tubal cilia loss in EOC.MethodsA whole exome sequencing (WES), immunohistochemistry (IHC), western blot, and reverse transcription quantitative (RT q) PCR were performed in 80 patients with EOC and 50 cases of non ovarian cancer to detect the mutations and expression of tubal ciliary marker, ciliary morphology, and abnormal rate.ResultsWe found that the incidence of tubal cilia loss was higher in EOC group with decreased expression of HYDIN compared with the control group (P<0.05).DiscussionThis study suggests that tubal ciliary loss is evident in epithelial fallopian tube carcinoma, and ciliary cells may be involved in the occurrence and development of EOC, and cilia-related gene HYDIN is expected to be a tumor marker for epithelial ovarian cancer.https://www.frontiersin.org/articles/10.3389/fonc.2024.1495753/fullfallopian tubesciliaepithelial ovarian cancerHYDINmarker |
| spellingShingle | Yuanli Guo Yuanli Guo Xinxin He Junfeng Liu Yanming Tan Chao Zhang Shan Chen Shan Chen Sheng Zhang The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer Frontiers in Oncology fallopian tubes cilia epithelial ovarian cancer HYDIN marker |
| title | The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| title_full | The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| title_fullStr | The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| title_full_unstemmed | The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| title_short | The relationship between HYDIN and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| title_sort | relationship between hydin and fallopian tubal cilia loss in patients with epithelial ovarian cancer |
| topic | fallopian tubes cilia epithelial ovarian cancer HYDIN marker |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2024.1495753/full |
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