Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus
Background. The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism. Methods. Sprague-Dawley rats were randomized into five groups (6/group): the con...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/924296 |
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| author | Shanjun Tan Wenkui Yu Zhiliang Lin Qiyi Chen Jialiang Shi Yi Dong Kaipeng Duan Xiaowu Bai Lin Xu Jieshou Li Ning Li |
| author_facet | Shanjun Tan Wenkui Yu Zhiliang Lin Qiyi Chen Jialiang Shi Yi Dong Kaipeng Duan Xiaowu Bai Lin Xu Jieshou Li Ning Li |
| author_sort | Shanjun Tan |
| collection | DOAJ |
| description | Background. The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism. Methods. Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC). Results. Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC. Conclusions. The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response. |
| format | Article |
| id | doaj-art-fbcb4b035482420795eb7cb207a0936b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-fbcb4b035482420795eb7cb207a0936b2025-02-03T05:53:06ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/924296924296Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative IleusShanjun Tan0Wenkui Yu1Zhiliang Lin2Qiyi Chen3Jialiang Shi4Yi Dong5Kaipeng Duan6Xiaowu Bai7Lin Xu8Jieshou Li9Ning Li10Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Clinical School of Nanjing, Second Military Medical University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaResearch Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, 305 East Zhongshan Road, Nanjing 210002, ChinaBackground. The pathogenesis of postoperative ileus (POI) is complex. The present study was designed to investigate the effects of peritoneal air exposure on the POI intestinal inflammation and the underlying mechanism. Methods. Sprague-Dawley rats were randomized into five groups (6/group): the control group, the sham group, and three exposure groups with peritoneal air exposure for 1, 2, or 3 h. At 24 h after surgery, we analyzed the gastrointestinal transit, the serum levels of tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-10, the myeloperoxidase activity, and the levels of TNF-α, IL-1β, IL-6, and IL-10 in the ileum and colon. The oxidant and antioxidant levels in the ileum and colon were analyzed by measuring malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC). Results. Peritoneal air exposure caused an air-exposure-time-dependent decrease in the gastrointestinal transit. The length of peritoneal air exposure is correlated with the severity of both systemic and intestinal inflammations and the increases in the levels of MDA, SOD, GSH-Px, and T-AOC. Conclusions. The length of peritoneal air exposure is proportional to the degree of intestinal paralysis and the severity of intestinal inflammation, which is linked to the oxidative stress response.http://dx.doi.org/10.1155/2014/924296 |
| spellingShingle | Shanjun Tan Wenkui Yu Zhiliang Lin Qiyi Chen Jialiang Shi Yi Dong Kaipeng Duan Xiaowu Bai Lin Xu Jieshou Li Ning Li Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus Mediators of Inflammation |
| title | Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus |
| title_full | Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus |
| title_fullStr | Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus |
| title_full_unstemmed | Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus |
| title_short | Peritoneal Air Exposure Elicits an Intestinal Inflammation Resulting in Postoperative Ileus |
| title_sort | peritoneal air exposure elicits an intestinal inflammation resulting in postoperative ileus |
| url | http://dx.doi.org/10.1155/2014/924296 |
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