A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat
The prevalence of obesity increases yearly in the world. The traditional local diet of the Western Regions of Cameroon was suspected to be the main contributor to the high prevalence of obesity in these Regions. This study aimed to evaluate the effects of a Cameroon-comparable fat diet on visceral o...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024170429 |
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| author | Sandrine Nkoubat Tchoundjwen Armel Georges Kamgang Tchawou Clémence Mvongo Adamou Mfopa Joseph Ngakou Mukam Paul Aimé Noubissi Gaetan Olivier Fankem René Kamgang Jean Louis Essame Oyono |
| author_facet | Sandrine Nkoubat Tchoundjwen Armel Georges Kamgang Tchawou Clémence Mvongo Adamou Mfopa Joseph Ngakou Mukam Paul Aimé Noubissi Gaetan Olivier Fankem René Kamgang Jean Louis Essame Oyono |
| author_sort | Sandrine Nkoubat Tchoundjwen |
| collection | DOAJ |
| description | The prevalence of obesity increases yearly in the world. The traditional local diet of the Western Regions of Cameroon was suspected to be the main contributor to the high prevalence of obesity in these Regions. This study aimed to evaluate the effects of a Cameroon-comparable fat diet on visceral obesity in rats.Two groups of male Wistar rats were fed for four months with respectively a normal diet (ND) (3400 kcal/kg of food) and a high-fat diet (HFD) containing maize, cassava, palm oil, and sugar (MACAPOS 2): 35 % carbohydrate, 55 % fat and 10 % proteins (4730 kcal/kg of food). Lee index, body weight, food intake, blood and hepatic lipids, body fat, insulin resistance, glucose tolerance, glycemia, serum insulin, leptin, and adiponectin were evaluated.HFD significantly (P < 0.01) increased body weight and decreased food intake. After four months of diet, 88.8 % of HFD rats were obese (Lee index >30 g/cm), and HFD significantly increased visceral and subcutaneous fats compared to ND. HFD increased triglyceride, total cholesterol, Low-density lipoprotein-cholesterol levels, and the atherogenic index, while the high-density lipoprotein-cholesterol level was decreased. The hepatic triglyceride and total cholesterol levels significantly (P < 0.01) increased in HFD, compared to ND. In HFD, the fasting blood glucose, serum insulin, and leptin levels significantly (P < 0.01) increased, meanwhile adiponectin decreased. HFD-induced glucose intolerance and insulin resistance in rats.Based on our findings, we can conclude that HFD MACAPOS 2 can induce central obesity. Therefore, it can be used as a model of diet-induced obesity. |
| format | Article |
| id | doaj-art-fbc127002a38449c8c7b343fe61b77ca |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Heliyon |
| spelling | doaj-art-fbc127002a38449c8c7b343fe61b77ca2025-01-17T04:49:59ZengElsevierHeliyon2405-84402025-01-01111e41011A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in ratSandrine Nkoubat Tchoundjwen0Armel Georges Kamgang Tchawou1Clémence Mvongo2Adamou Mfopa3Joseph Ngakou Mukam4Paul Aimé Noubissi5Gaetan Olivier Fankem6René Kamgang7Jean Louis Essame Oyono8Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonLaboratory of Human Metabolism and Non-Communicable Diseases, Research Centre on Health and Priority Pathologies, (IMPM), P.O. Box. 13033, Yaoundé, CameroonDepartment of Life Science, Higher Teacher Training College, University of Bertoua, CameroonLaboratory of Phytoprotection and Valorization of Genetics Resources, Biotechnology Centre Nkolbisson, P.O. Box 17673 Etetak, Yaoundé, Cameroon; Laboratory of Human Metabolism and Non-Communicable Diseases, Research Centre on Health and Priority Pathologies, (IMPM), P.O. Box. 13033, Yaoundé, CameroonDepartment of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonDepartment of Zoology and Animal Physiology, Faculty of Science, University of Buea, Cameroon; Corresponding author.Department of Animal Biology and Physiology, Faculty of Science, University of Yaoundé I, P.O. Box 812, Yaoundé, CameroonLaboratory of Human Metabolism and Non-Communicable Diseases, Research Centre on Health and Priority Pathologies, (IMPM), P.O. Box. 13033, Yaoundé, CameroonLaboratory of Human Metabolism and Non-Communicable Diseases, Research Centre on Health and Priority Pathologies, (IMPM), P.O. Box. 13033, Yaoundé, CameroonThe prevalence of obesity increases yearly in the world. The traditional local diet of the Western Regions of Cameroon was suspected to be the main contributor to the high prevalence of obesity in these Regions. This study aimed to evaluate the effects of a Cameroon-comparable fat diet on visceral obesity in rats.Two groups of male Wistar rats were fed for four months with respectively a normal diet (ND) (3400 kcal/kg of food) and a high-fat diet (HFD) containing maize, cassava, palm oil, and sugar (MACAPOS 2): 35 % carbohydrate, 55 % fat and 10 % proteins (4730 kcal/kg of food). Lee index, body weight, food intake, blood and hepatic lipids, body fat, insulin resistance, glucose tolerance, glycemia, serum insulin, leptin, and adiponectin were evaluated.HFD significantly (P < 0.01) increased body weight and decreased food intake. After four months of diet, 88.8 % of HFD rats were obese (Lee index >30 g/cm), and HFD significantly increased visceral and subcutaneous fats compared to ND. HFD increased triglyceride, total cholesterol, Low-density lipoprotein-cholesterol levels, and the atherogenic index, while the high-density lipoprotein-cholesterol level was decreased. The hepatic triglyceride and total cholesterol levels significantly (P < 0.01) increased in HFD, compared to ND. In HFD, the fasting blood glucose, serum insulin, and leptin levels significantly (P < 0.01) increased, meanwhile adiponectin decreased. HFD-induced glucose intolerance and insulin resistance in rats.Based on our findings, we can conclude that HFD MACAPOS 2 can induce central obesity. Therefore, it can be used as a model of diet-induced obesity.http://www.sciencedirect.com/science/article/pii/S2405844024170429AdiponectinCentral obesityHigh-fat dietInsulinLeptinMACAPOS 2 |
| spellingShingle | Sandrine Nkoubat Tchoundjwen Armel Georges Kamgang Tchawou Clémence Mvongo Adamou Mfopa Joseph Ngakou Mukam Paul Aimé Noubissi Gaetan Olivier Fankem René Kamgang Jean Louis Essame Oyono A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat Heliyon Adiponectin Central obesity High-fat diet Insulin Leptin MACAPOS 2 |
| title | A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat |
| title_full | A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat |
| title_fullStr | A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat |
| title_full_unstemmed | A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat |
| title_short | A Cameroon Western Regions high-fat diet (MACAPOS 2) induces visceral obesity in rat |
| title_sort | cameroon western regions high fat diet macapos 2 induces visceral obesity in rat |
| topic | Adiponectin Central obesity High-fat diet Insulin Leptin MACAPOS 2 |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024170429 |
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