Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform
<b>Background:</b> Circulating tumor cells (CTCs) have recently been developed as biomarkers. Several studies have reported on the clinical use of CTCs to assess drug resistance in various cancers. However, sequential and multiple CTC measurements during chemotherapy are relatively rare....
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MDPI AG
2025-03-01
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| author | Kohei Yasui Takuya Saito Sho Ueda Kentaro Shinohara Yasuyuki Fukami Tsuyoshi Sano Hayao Nakanishi |
| author_facet | Kohei Yasui Takuya Saito Sho Ueda Kentaro Shinohara Yasuyuki Fukami Tsuyoshi Sano Hayao Nakanishi |
| author_sort | Kohei Yasui |
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| description | <b>Background:</b> Circulating tumor cells (CTCs) have recently been developed as biomarkers. Several studies have reported on the clinical use of CTCs to assess drug resistance in various cancers. However, sequential and multiple CTC measurements during chemotherapy are relatively rare. We recently reported a transient increase in CTCs early after chemotherapy by sequentially detecting CTCs in a human pancreatic cancer xenograft model in nude mice. <b>Method</b>: In the present study, using a newly developed immunocytology and glass slide-based convenient CTC detection platform, we examined CTC numbers sequentially before, during, and after chemotherapy in the peripheral blood of 14 pancreatic cancer patients, pathological stage (pStage) I-IV, who underwent surgery with preoperative chemotherapy and GS (Gem/S-1) and GnP (Gem/nab-PTX). <b>Results:</b> Among patients with strongly or weakly elevated CTC counts (3–44/5 mL of blood) following GS treatment, four out of six pancreatic cancer patients were judged to have a partial response (PR), and two out of six were deemed to have stable disease (SD) as a clinical response based on the CT image. In contrast, in patients with GnP therapy, three out of four patients showed no CTC response, and these three patients were judged to have progressive disease (PD), while the remaining one patient was judged to have SD in terms of their clinical response. <b>Conclusion</b>: These results suggest that sequential CTC monitoring during preoperative chemotherapy in pancreatic cancer patients can be a helpful liquid biopsy diagnostic tool as a therapeutic marker to predict tumor chemosensitivity and chemoresistance in clinical settings. Further large-scale clinical studies are required to confirm and clarify this hypothesis. |
| format | Article |
| id | doaj-art-fbb53e83c46e4d7bb4ff3d4914cd055e |
| institution | Kabale University |
| issn | 2075-4418 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj-art-fbb53e83c46e4d7bb4ff3d4914cd055e2025-08-20T03:43:37ZengMDPI AGDiagnostics2075-44182025-03-0115675210.3390/diagnostics15060752Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection PlatformKohei Yasui0Takuya Saito1Sho Ueda2Kentaro Shinohara3Yasuyuki Fukami4Tsuyoshi Sano5Hayao Nakanishi6Department of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, JapanDepartment of Gastroenterological Surgery, Graduate School of Medicine, Aichi Medical University, Nagoya 480-1195, Japan<b>Background:</b> Circulating tumor cells (CTCs) have recently been developed as biomarkers. Several studies have reported on the clinical use of CTCs to assess drug resistance in various cancers. However, sequential and multiple CTC measurements during chemotherapy are relatively rare. We recently reported a transient increase in CTCs early after chemotherapy by sequentially detecting CTCs in a human pancreatic cancer xenograft model in nude mice. <b>Method</b>: In the present study, using a newly developed immunocytology and glass slide-based convenient CTC detection platform, we examined CTC numbers sequentially before, during, and after chemotherapy in the peripheral blood of 14 pancreatic cancer patients, pathological stage (pStage) I-IV, who underwent surgery with preoperative chemotherapy and GS (Gem/S-1) and GnP (Gem/nab-PTX). <b>Results:</b> Among patients with strongly or weakly elevated CTC counts (3–44/5 mL of blood) following GS treatment, four out of six pancreatic cancer patients were judged to have a partial response (PR), and two out of six were deemed to have stable disease (SD) as a clinical response based on the CT image. In contrast, in patients with GnP therapy, three out of four patients showed no CTC response, and these three patients were judged to have progressive disease (PD), while the remaining one patient was judged to have SD in terms of their clinical response. <b>Conclusion</b>: These results suggest that sequential CTC monitoring during preoperative chemotherapy in pancreatic cancer patients can be a helpful liquid biopsy diagnostic tool as a therapeutic marker to predict tumor chemosensitivity and chemoresistance in clinical settings. Further large-scale clinical studies are required to confirm and clarify this hypothesis.https://www.mdpi.com/2075-4418/15/6/752circulating tumor cells (CTCs)cytology-based CTC detectionpreoperatve chemotherapypancreatic cancertherapeutic marker |
| spellingShingle | Kohei Yasui Takuya Saito Sho Ueda Kentaro Shinohara Yasuyuki Fukami Tsuyoshi Sano Hayao Nakanishi Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform Diagnostics circulating tumor cells (CTCs) cytology-based CTC detection preoperatve chemotherapy pancreatic cancer therapeutic marker |
| title | Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform |
| title_full | Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform |
| title_fullStr | Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform |
| title_full_unstemmed | Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform |
| title_short | Sequential Changes in Circulating Tumor Cells in the Peripheral Blood of Pancreatic Cancer Patients with Preoperative Chemotherapy Using a New Immunocytology-Based, Light Microscopic CTC Detection Platform |
| title_sort | sequential changes in circulating tumor cells in the peripheral blood of pancreatic cancer patients with preoperative chemotherapy using a new immunocytology based light microscopic ctc detection platform |
| topic | circulating tumor cells (CTCs) cytology-based CTC detection preoperatve chemotherapy pancreatic cancer therapeutic marker |
| url | https://www.mdpi.com/2075-4418/15/6/752 |
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