Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues

Comparative evaluation of ACetic - MEthanol high salt dissociation approach for single-cell transcriptomics of frozen human tissues

Current dissociation methods for solid tissues in scRNA-seq studies do not guarantee intact single-cell isolation, especially for sensitive and complex human endocrine tissues. Most studies rely on enzymatic dissociation of fresh samples or nuclei isolation from frozen samples. Dissociating whole in...

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Main Authors: Marina Utkina, Anastasia Shcherbakova, Ruslan Deviatiiarov, Alina Ryabova, Marina Loguinova, Valentin Trofimov, Anna Kuznetsova, Mikhail Petropavlovskiy, Rustam Salimkhanov, Denis Maksimov, Eugene Albert, Alexandra Golubeva, Walaa Asaad, Lilia Urusova, Ekaterina Bondarenko, Anastasia Lapshina, Alexandra Shutova, Dmitry Beltsevich, Oleg Gusev, Larisa Dzeranova, Galina Melnichenko, Ildar Minniakhmetov, Ivan Dedov, Natalya Mokrysheva, Sergey Popov
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
ScRNA-seq
ACME HS
cryopreservation
dissociation
fresh-frozen tissue
fixed single cells
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2024.1469955/full
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https://www.frontiersin.org/articles/10.3389/fcell.2024.1469955/full

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