Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City
Our previous research results showed that through microscopic and biochemical detection of single pure colonies,we obtained 45 positive samples of lactic acid bacteria isolated from cow milk in Kirkuk City In addition 40 isolates of pathogenic bacteria (10 isolates each of Staphylococcus aureus, Sta...
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| Language: | English |
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University of Kirkuk
2024-09-01
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| Series: | Kirkuk Journal of Science |
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| Online Access: | https://kujss.uokirkuk.edu.iq/article_184072_97c71f51d2dfb20c0da535fb85e2aec2.pdf |
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| author | Wasan Fahem Salah Zain Alabdeen Asal Tawfeeq |
| author_facet | Wasan Fahem Salah Zain Alabdeen Asal Tawfeeq |
| author_sort | Wasan Fahem |
| collection | DOAJ |
| description | Our previous research results showed that through microscopic and biochemical detection of single pure colonies,we obtained 45 positive samples of lactic acid bacteria isolated from cow milk in Kirkuk City In addition 40 isolates of pathogenic bacteria (10 isolates each of Staphylococcus aureus, Staphylococcus epidermidis,Pseudomonas aeruginosa and Escherichia coli) were obtained from Kirkuk City Hospitals during the period from December to February 2021.The results were displayed subsequent to their comparison with the non-inoculated culture medium. The same extraction procedures were conducted on this medium to assess the inhibitory effects of both the initial(supernatant)and final(deposits) extraction steps against two isolates of each gram-positive and gram-negative pathogenic bacteria. Consequently, we conducted molecular studies to evaluate the effectiveness of Lactobacillus isolates against these pathogenic strains. Geneious Prime 2021version 2.2 software was utilized to identify genes associated with bacteriocin production, specifically PlnK, PlnN, PlnEF, and PlW. |
| format | Article |
| id | doaj-art-fb8851adf4c24f6eb1d45352b41ff18d |
| institution | Kabale University |
| issn | 3005-4788 3005-4796 |
| language | English |
| publishDate | 2024-09-01 |
| publisher | University of Kirkuk |
| record_format | Article |
| series | Kirkuk Journal of Science |
| spelling | doaj-art-fb8851adf4c24f6eb1d45352b41ff18d2024-11-23T20:18:20ZengUniversity of KirkukKirkuk Journal of Science3005-47883005-47962024-09-011931610.32894/kujss.2024.147526.1147184072Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk CityWasan Fahem0Salah Zain Alabdeen1Asal Tawfeeq2Biology Department ,College of Education for Pure Science, University of Kirkuk , IraqBiology Department, college of education for Pure Science, University of Kirkuk, Kirkuk, Iraq.Therapeutic Nutrition Techniques Department , College of Health and Medical Technology, Northern Technical University, Kirkuk, Iraq>Our previous research results showed that through microscopic and biochemical detection of single pure colonies,we obtained 45 positive samples of lactic acid bacteria isolated from cow milk in Kirkuk City In addition 40 isolates of pathogenic bacteria (10 isolates each of Staphylococcus aureus, Staphylococcus epidermidis,Pseudomonas aeruginosa and Escherichia coli) were obtained from Kirkuk City Hospitals during the period from December to February 2021.The results were displayed subsequent to their comparison with the non-inoculated culture medium. The same extraction procedures were conducted on this medium to assess the inhibitory effects of both the initial(supernatant)and final(deposits) extraction steps against two isolates of each gram-positive and gram-negative pathogenic bacteria. Consequently, we conducted molecular studies to evaluate the effectiveness of Lactobacillus isolates against these pathogenic strains. Geneious Prime 2021version 2.2 software was utilized to identify genes associated with bacteriocin production, specifically PlnK, PlnN, PlnEF, and PlW.https://kujss.uokirkuk.edu.iq/article_184072_97c71f51d2dfb20c0da535fb85e2aec2.pdflactic acid bacteriabacteriocinlactiplantibacillus plantarum16srrnakirkuk |
| spellingShingle | Wasan Fahem Salah Zain Alabdeen Asal Tawfeeq Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City Kirkuk Journal of Science lactic acid bacteria bacteriocin lactiplantibacillus plantarum 16srrna kirkuk |
| title | Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City |
| title_full | Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City |
| title_fullStr | Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City |
| title_full_unstemmed | Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City |
| title_short | Identification of Genes Encoding the Production of Bacteriocin from Lactiplantibacillus Plantarum and its Activity Against Some Pathogenic Bacteria in Kirkuk City |
| title_sort | identification of genes encoding the production of bacteriocin from lactiplantibacillus plantarum and its activity against some pathogenic bacteria in kirkuk city |
| topic | lactic acid bacteria bacteriocin lactiplantibacillus plantarum 16srrna kirkuk |
| url | https://kujss.uokirkuk.edu.iq/article_184072_97c71f51d2dfb20c0da535fb85e2aec2.pdf |
| work_keys_str_mv | AT wasanfahem identificationofgenesencodingtheproductionofbacteriocinfromlactiplantibacillusplantarumanditsactivityagainstsomepathogenicbacteriainkirkukcity AT salahzainalabdeen identificationofgenesencodingtheproductionofbacteriocinfromlactiplantibacillusplantarumanditsactivityagainstsomepathogenicbacteriainkirkukcity AT asaltawfeeq identificationofgenesencodingtheproductionofbacteriocinfromlactiplantibacillusplantarumanditsactivityagainstsomepathogenicbacteriainkirkukcity |