Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot
Paracetamol (APAP) overdose is the leading cause of drug-induced liver injury, leading to acute liver failure. However, the role of concurrent acute or chronic ethanol ingestion in this context requires further clarification. In this study, we investigated the effects of acute and chronic ethanol in...
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2024-11-01
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| Online Access: | https://www.mdpi.com/2305-6304/12/12/857 |
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| author | Allan Cristian Gonçalves Aline Meireles Coelho Maria Laura da Cruz Castro Renata Rebeca Pereira Natalia Pereira da Silva Araújo Flávia Monteiro Ferreira Pedro Alves Machado Júnior Sirlaine Pio Camilo Elber Vital Frank Silva Bezerra André Talvani William de Castro Borges Emerson Cruz de Oliveira Daniela Caldeira Costa |
| author_facet | Allan Cristian Gonçalves Aline Meireles Coelho Maria Laura da Cruz Castro Renata Rebeca Pereira Natalia Pereira da Silva Araújo Flávia Monteiro Ferreira Pedro Alves Machado Júnior Sirlaine Pio Camilo Elber Vital Frank Silva Bezerra André Talvani William de Castro Borges Emerson Cruz de Oliveira Daniela Caldeira Costa |
| author_sort | Allan Cristian Gonçalves |
| collection | DOAJ |
| description | Paracetamol (APAP) overdose is the leading cause of drug-induced liver injury, leading to acute liver failure. However, the role of concurrent acute or chronic ethanol ingestion in this context requires further clarification. In this study, we investigated the effects of acute and chronic ethanol ingestion on APAP-induced hepatotoxicity. Male C57BL/6 mice were randomly allocated into four groups: control (C; water 2×/day for 7 days); APAP (single dose of APAP, 500 mg/kg); acute ethanol (AE; a single ethanol dose—10 mL/kg, and one hour later an overdose of APAP—500 mg/kg); chronic ethanol (CE; ethanol—10 mL/kg, 2×/day for 7 days; and on the last day, an overdose of APAP—500 mg/kg). The results showed that AE induced heightened liver damage, increased necrotic area, and elevated levels of ALT, AST, TBARS, and oxidized glutathione compared to the control group. The AE group exhibited diminished glutathione availability and elevated CYP2E1 levels compared to the other groups. CE maintained a hepatic profile similar to that of the control group in terms of necrosis index, ALT and AST levels, GSH/GSSG ratio, and CYP2E1 activity, along with the upregulation of gene expression of the glucuronidation enzyme compared to the APAP group. Proteomic analysis revealed that the AE protein profile closely resembled that of the APAP group, whereas the C and CE groups were clustered together. In conclusion, ethanol consumption differentially modulated APAP overdose-induced liver damage. Acute consumption exacerbated hepatotoxicity, similar to an APAP overdose alone, whereas chronic consumption appeared to mitigate this injury, at least within the parameters assessed in this study. |
| format | Article |
| id | doaj-art-fb832ac7a954418a96f2f4225515e6dc |
| institution | Kabale University |
| issn | 2305-6304 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
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| series | Toxics |
| spelling | doaj-art-fb832ac7a954418a96f2f4225515e6dc2024-12-27T14:56:37ZengMDPI AGToxics2305-63042024-11-01121285710.3390/toxics12120857Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study PilotAllan Cristian Gonçalves0Aline Meireles Coelho1Maria Laura da Cruz Castro2Renata Rebeca Pereira3Natalia Pereira da Silva Araújo4Flávia Monteiro Ferreira5Pedro Alves Machado Júnior6Sirlaine Pio7Camilo Elber Vital8Frank Silva Bezerra9André Talvani10William de Castro Borges11Emerson Cruz de Oliveira12Daniela Caldeira Costa13Laboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Experimental Pathophysiology, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Immunobiology of Inflammation, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35400-000, MG, BrazilLaboratory of Enzymology and Proteomics, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Experimental Pathophysiology, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Immunobiology of Inflammation, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35400-000, MG, BrazilLaboratory of Enzymology and Proteomics, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilLaboratory of Exercise of Physiology, School of Physical Education, UFOP, Ouro Preto 35400-000, MG, BrazilLaboratory of Metabolic Biochemistry, Institute of Exact and Biological Sciences, UFOP, Ouro Preto 35402-136, MG, BrazilParacetamol (APAP) overdose is the leading cause of drug-induced liver injury, leading to acute liver failure. However, the role of concurrent acute or chronic ethanol ingestion in this context requires further clarification. In this study, we investigated the effects of acute and chronic ethanol ingestion on APAP-induced hepatotoxicity. Male C57BL/6 mice were randomly allocated into four groups: control (C; water 2×/day for 7 days); APAP (single dose of APAP, 500 mg/kg); acute ethanol (AE; a single ethanol dose—10 mL/kg, and one hour later an overdose of APAP—500 mg/kg); chronic ethanol (CE; ethanol—10 mL/kg, 2×/day for 7 days; and on the last day, an overdose of APAP—500 mg/kg). The results showed that AE induced heightened liver damage, increased necrotic area, and elevated levels of ALT, AST, TBARS, and oxidized glutathione compared to the control group. The AE group exhibited diminished glutathione availability and elevated CYP2E1 levels compared to the other groups. CE maintained a hepatic profile similar to that of the control group in terms of necrosis index, ALT and AST levels, GSH/GSSG ratio, and CYP2E1 activity, along with the upregulation of gene expression of the glucuronidation enzyme compared to the APAP group. Proteomic analysis revealed that the AE protein profile closely resembled that of the APAP group, whereas the C and CE groups were clustered together. In conclusion, ethanol consumption differentially modulated APAP overdose-induced liver damage. Acute consumption exacerbated hepatotoxicity, similar to an APAP overdose alone, whereas chronic consumption appeared to mitigate this injury, at least within the parameters assessed in this study.https://www.mdpi.com/2305-6304/12/12/857paracetamolacetaminophenethanolalcoholoxidative stresshepatotoxicity |
| spellingShingle | Allan Cristian Gonçalves Aline Meireles Coelho Maria Laura da Cruz Castro Renata Rebeca Pereira Natalia Pereira da Silva Araújo Flávia Monteiro Ferreira Pedro Alves Machado Júnior Sirlaine Pio Camilo Elber Vital Frank Silva Bezerra André Talvani William de Castro Borges Emerson Cruz de Oliveira Daniela Caldeira Costa Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot Toxics paracetamol acetaminophen ethanol alcohol oxidative stress hepatotoxicity |
| title | Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot |
| title_full | Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot |
| title_fullStr | Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot |
| title_full_unstemmed | Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot |
| title_short | Modulation of Paracetamol-Induced Hepatotoxicity by Acute and Chronic Ethanol Consumption in Mice: A Study Pilot |
| title_sort | modulation of paracetamol induced hepatotoxicity by acute and chronic ethanol consumption in mice a study pilot |
| topic | paracetamol acetaminophen ethanol alcohol oxidative stress hepatotoxicity |
| url | https://www.mdpi.com/2305-6304/12/12/857 |
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