Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress

Heat stress poses a significant challenge to agricultural productivity, particularly in pepper plants, by affecting their physiological and biochemical processes. Proline, an amino acid known for its role in stress mitigation, has shown potential in alleviating such impacts. However, its efficacy sp...

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Main Authors: Zeinab Masoumi, Saeed Khosravi, Maryam Haghighi, Maryam Mozafarian
Format: Article
Language:English
Published: Elsevier 2024-12-01
Series:Plant Stress
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667064X24003488
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author Zeinab Masoumi
Saeed Khosravi
Maryam Haghighi
Maryam Mozafarian
author_facet Zeinab Masoumi
Saeed Khosravi
Maryam Haghighi
Maryam Mozafarian
author_sort Zeinab Masoumi
collection DOAJ
description Heat stress poses a significant challenge to agricultural productivity, particularly in pepper plants, by affecting their physiological and biochemical processes. Proline, an amino acid known for its role in stress mitigation, has shown potential in alleviating such impacts. However, its efficacy specifically under heat stress conditions in pepper plants has not been well established. This study aims to investigate whether exogenous application of proline and its encapsulated form can effectively reduce the adverse effects of high temperatures compared to optimal conditions. The treatments involved two temperature conditions: 25 ± 2 °C (CT) and 40 ± 2 °C (HT), along with various proline sources, including distilled water (C), proline (Pr), and encapsulated proline (N-Pr), each at a concentration of 20 mM. The findings of this study revealed that high-temperature stress led to a decrease in PIP1 gene expression which plays a crucial role in regulating water transport and maintaining cellular hydration, and linoleic acid content in pepper plants. The application of proline resulted in improved growth traits under optimal temperature in comparison with control and N-Pr. Furthermore, electrolyte leakage (EL) decreased by 45 % with the application of Pr under high temperature compared to control. Under heat stress, the application of N-Pr enhanced the chlorophyll index, phenol, and proline concentration by 24 %, 102 %, and 147 % respectively. Overall, the use of Pr under optimal temperature and N-Pr under high temperature could improve the resistance of pepper plants.
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spelling doaj-art-fb5deb456a7e4fbfbc6849cedca7cf652024-12-19T11:02:15ZengElsevierPlant Stress2667-064X2024-12-0114100695Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stressZeinab Masoumi0Saeed Khosravi1Maryam Haghighi2Maryam Mozafarian3Department of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, IranDepartment of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, IranDepartment of Horticulture, College of Agriculture, Isfahan University of Technology, Isfahan, Iran; Corresponding authors.Department of Vegetable and Mushroom Growing, Hungarian University of Agriculture and Life Sciences, Budapest, Hungary; Corresponding authors.Heat stress poses a significant challenge to agricultural productivity, particularly in pepper plants, by affecting their physiological and biochemical processes. Proline, an amino acid known for its role in stress mitigation, has shown potential in alleviating such impacts. However, its efficacy specifically under heat stress conditions in pepper plants has not been well established. This study aims to investigate whether exogenous application of proline and its encapsulated form can effectively reduce the adverse effects of high temperatures compared to optimal conditions. The treatments involved two temperature conditions: 25 ± 2 °C (CT) and 40 ± 2 °C (HT), along with various proline sources, including distilled water (C), proline (Pr), and encapsulated proline (N-Pr), each at a concentration of 20 mM. The findings of this study revealed that high-temperature stress led to a decrease in PIP1 gene expression which plays a crucial role in regulating water transport and maintaining cellular hydration, and linoleic acid content in pepper plants. The application of proline resulted in improved growth traits under optimal temperature in comparison with control and N-Pr. Furthermore, electrolyte leakage (EL) decreased by 45 % with the application of Pr under high temperature compared to control. Under heat stress, the application of N-Pr enhanced the chlorophyll index, phenol, and proline concentration by 24 %, 102 %, and 147 % respectively. Overall, the use of Pr under optimal temperature and N-Pr under high temperature could improve the resistance of pepper plants.http://www.sciencedirect.com/science/article/pii/S2667064X24003488Abiotic stressAmino acidEncapsulated prolineHigh temperaturePhenolic content
spellingShingle Zeinab Masoumi
Saeed Khosravi
Maryam Haghighi
Maryam Mozafarian
Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
Plant Stress
Abiotic stress
Amino acid
Encapsulated proline
High temperature
Phenolic content
title Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
title_full Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
title_fullStr Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
title_full_unstemmed Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
title_short Exploring the protective effects of proline on pepper (Capsicum annum L.) under high-temperature stress
title_sort exploring the protective effects of proline on pepper capsicum annum l under high temperature stress
topic Abiotic stress
Amino acid
Encapsulated proline
High temperature
Phenolic content
url http://www.sciencedirect.com/science/article/pii/S2667064X24003488
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