Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp.
Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with dif...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
EDP Sciences
2024-01-01
|
| Series: | BIO Web of Conferences |
| Online Access: | https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01028.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841526848577601536 |
|---|---|
| author | Daniel Manurung Josua Uju Santoso Joko Sudiyani Yanni Maryana Roni Chasanah Ekowati |
| author_facet | Daniel Manurung Josua Uju Santoso Joko Sudiyani Yanni Maryana Roni Chasanah Ekowati |
| author_sort | Daniel Manurung Josua |
| collection | DOAJ |
| description | Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with different time variations, namely 60, 90, and 120 minutes at a temperature of 50oC. After pretreatment, the enzymatic hydrolysis process was carried out using cellulase enzymes on a shaker incubator at a temperature of 50oC, 150 rpm for 72 hours, then the fermentation process for 72 hours using Saccharomices cerevisiae mold. The results showed that 5% NaOH alkali pretreatment could improve the quality of Gracilaria sp. biomass. The lignin content produced was 10.43 ± 0.15%. |
| format | Article |
| id | doaj-art-fb3136928e3d43249f5c8d9349548251 |
| institution | Kabale University |
| issn | 2117-4458 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | BIO Web of Conferences |
| spelling | doaj-art-fb3136928e3d43249f5c8d93495482512025-01-16T11:19:24ZengEDP SciencesBIO Web of Conferences2117-44582024-01-011470102810.1051/bioconf/202414701028bioconf_eafta2024_01028Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp.Daniel Manurung Josua0Uju1Santoso Joko2Sudiyani Yanni3Maryana Roni4Chasanah Ekowati5Department of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Jl Agatis Kampus IPB DramagaDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Jl Agatis Kampus IPB DramagaDepartment of Aquatic Product Technology, Faculty of Fisheries and Marine Sciences, IPB University, Jl Agatis Kampus IPB DramagaResearch Center for Chemistry-National Research and nnovation Agency Indonesia (BRIN)Research Center for Chemistry-National Research and nnovation Agency Indonesia (BRIN)Research Center for Marine and Land Bioindustry-National Research and Innovation Agency (BRIN)Pretreatment of seaweed biomass for bioethanol production. This study aimed to determine the effect of biomass pretreatment with 5% NaOH alkali on enzymatic hydrolysis in the bioethanol production process using Gracilaria sp. as raw material. Pretreatment with 5% NaOH alkali was carried out with different time variations, namely 60, 90, and 120 minutes at a temperature of 50oC. After pretreatment, the enzymatic hydrolysis process was carried out using cellulase enzymes on a shaker incubator at a temperature of 50oC, 150 rpm for 72 hours, then the fermentation process for 72 hours using Saccharomices cerevisiae mold. The results showed that 5% NaOH alkali pretreatment could improve the quality of Gracilaria sp. biomass. The lignin content produced was 10.43 ± 0.15%.https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01028.pdf |
| spellingShingle | Daniel Manurung Josua Uju Santoso Joko Sudiyani Yanni Maryana Roni Chasanah Ekowati Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. BIO Web of Conferences |
| title | Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. |
| title_full | Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. |
| title_fullStr | Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. |
| title_full_unstemmed | Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. |
| title_short | Effect of Alkali Pretreatment on Enzymatic Hydrolysis of Gracilaria sp. |
| title_sort | effect of alkali pretreatment on enzymatic hydrolysis of gracilaria sp |
| url | https://www.bio-conferences.org/articles/bioconf/pdf/2024/66/bioconf_eafta2024_01028.pdf |
| work_keys_str_mv | AT danielmanurungjosua effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp AT uju effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp AT santosojoko effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp AT sudiyaniyanni effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp AT maryanaroni effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp AT chasanahekowati effectofalkalipretreatmentonenzymatichydrolysisofgracilariasp |