Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring
The Asian Arowana, Scleropages formosus (Müller and Schlegel, 1844) is a large majestic freshwater teleost, crowned as the king of aquariums with its bright charismatic appearance and magnificent swimming performance. The most expensive Asian arowana is the Golden Blue-based Malayan Arowana which is...
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Elsevier
2025-06-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215016124005843 |
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| author | Nurul Affiqah Edora Mohd-Azhar Haslawati Baharuddin Mohamad-Sufiyan Salmi Noor Faizah Ismail Amatul-Samahah Md. Ali Syazwan Saidin Adibah Abu-Bakar |
| author_facet | Nurul Affiqah Edora Mohd-Azhar Haslawati Baharuddin Mohamad-Sufiyan Salmi Noor Faizah Ismail Amatul-Samahah Md. Ali Syazwan Saidin Adibah Abu-Bakar |
| author_sort | Nurul Affiqah Edora Mohd-Azhar |
| collection | DOAJ |
| description | The Asian Arowana, Scleropages formosus (Müller and Schlegel, 1844) is a large majestic freshwater teleost, crowned as the king of aquariums with its bright charismatic appearance and magnificent swimming performance. The most expensive Asian arowana is the Golden Blue-based Malayan Arowana which is endemic to the Bukit Merah Lake and Kerian River Basin, Perak, Malaysia. S. formosus has been listed as endangered by the IUCN (International Union for Conservation of Nature), regulated under Appendix 1 of the Convention of International Trade on Endangered Species (CITES) for commercial trade. Environmental DNA (eDNA) analysis has become widely accepted in biodiversity monitoring for the detection of rare and endangered species without harming any ecosystem or threatened species. Hence, the application of eDNA as wild population monitoring of S. formosus is possible for conservation and CITES enforcement program. • The species-specific primer of S. formosus was designed based on selected sequences obtained from GenBank • This report presents the potential application of eDNA in the management of the Malaysian 686 CITES Act for conservation monitoring of the Asian Arowana • The detection and wild population monitoring is possible through the eDNA method as complementary tools |
| format | Article |
| id | doaj-art-fac447050f0947a8b795736e62f49457 |
| institution | Kabale University |
| issn | 2215-0161 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | MethodsX |
| spelling | doaj-art-fac447050f0947a8b795736e62f494572025-01-06T04:08:41ZengElsevierMethodsX2215-01612025-06-0114103133Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoringNurul Affiqah Edora Mohd-Azhar0Haslawati Baharuddin1Mohamad-Sufiyan Salmi2Noor Faizah Ismail3Amatul-Samahah Md. Ali4Syazwan Saidin5Adibah Abu-Bakar6Department of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), Tanjong Malim, Perak 35900, MalaysiaFisheries Research Institute (FRI) Glami Lemi, Jelebu, Negeri Sembilan 71650, Malaysia; Corresponding authors.Fisheries Research Institute (FRI) Glami Lemi, Jelebu, Negeri Sembilan 71650, MalaysiaFisheries Research Institute (FRI) Glami Lemi, Jelebu, Negeri Sembilan 71650, MalaysiaFisheries Research Institute (FRI) Glami Lemi, Jelebu, Negeri Sembilan 71650, MalaysiaDepartment of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), Tanjong Malim, Perak 35900, MalaysiaDepartment of Biology, Faculty of Science and Mathematics, Universiti Pendidikan Sultan Idris (UPSI), Tanjong Malim, Perak 35900, Malaysia; Corresponding authors.The Asian Arowana, Scleropages formosus (Müller and Schlegel, 1844) is a large majestic freshwater teleost, crowned as the king of aquariums with its bright charismatic appearance and magnificent swimming performance. The most expensive Asian arowana is the Golden Blue-based Malayan Arowana which is endemic to the Bukit Merah Lake and Kerian River Basin, Perak, Malaysia. S. formosus has been listed as endangered by the IUCN (International Union for Conservation of Nature), regulated under Appendix 1 of the Convention of International Trade on Endangered Species (CITES) for commercial trade. Environmental DNA (eDNA) analysis has become widely accepted in biodiversity monitoring for the detection of rare and endangered species without harming any ecosystem or threatened species. Hence, the application of eDNA as wild population monitoring of S. formosus is possible for conservation and CITES enforcement program. • The species-specific primer of S. formosus was designed based on selected sequences obtained from GenBank • This report presents the potential application of eDNA in the management of the Malaysian 686 CITES Act for conservation monitoring of the Asian Arowana • The detection and wild population monitoring is possible through the eDNA method as complementary toolshttp://www.sciencedirect.com/science/article/pii/S2215016124005843Development of species-specific eDNA primer for the detection of the Asian arowana (Scleropages formosus) |
| spellingShingle | Nurul Affiqah Edora Mohd-Azhar Haslawati Baharuddin Mohamad-Sufiyan Salmi Noor Faizah Ismail Amatul-Samahah Md. Ali Syazwan Saidin Adibah Abu-Bakar Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring MethodsX Development of species-specific eDNA primer for the detection of the Asian arowana (Scleropages formosus) |
| title | Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring |
| title_full | Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring |
| title_fullStr | Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring |
| title_full_unstemmed | Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring |
| title_short | Development and validation of a species-specific environmental DNA (eDNA) primer for endangered Asian arowana, Scleropages formosus (Teleostei: Osteoglossidae) for law enforcement and wild population monitoring |
| title_sort | development and validation of a species specific environmental dna edna primer for endangered asian arowana scleropages formosus teleostei osteoglossidae for law enforcement and wild population monitoring |
| topic | Development of species-specific eDNA primer for the detection of the Asian arowana (Scleropages formosus) |
| url | http://www.sciencedirect.com/science/article/pii/S2215016124005843 |
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