Unveiling the invisible genomic dynamics
Abstract CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these techniques, highlighting key strateg...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Publishing Group
2025-07-01
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| Series: | Experimental and Molecular Medicine |
| Online Access: | https://doi.org/10.1038/s12276-025-01434-z |
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| _version_ | 1849238040895029248 |
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| author | Jiwoong Kwon Yeji Park Taekjip Ha |
| author_facet | Jiwoong Kwon Yeji Park Taekjip Ha |
| author_sort | Jiwoong Kwon |
| collection | DOAJ |
| description | Abstract CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these techniques, highlighting key strategies such as signal amplification, background reduction, multiplexing and enhanced genomic resolution. By engineering Cas proteins and guide RNAs, and incorporating peptide and aptamer tags, researchers have remarkably improved the sensitivity, specificity and resolution of CRISPR-based imaging, enabling the detection of nonrepetitive genomic regions and single-nucleotide polymorphisms. Recent studies have further pushed the boundaries of CRISPR-based imaging with the introduction of degron-mediated fluorogenic labeling and light-controllable background reduction. Despite remaining challenges, such as the bulkiness of signal amplification systems, limitations in guide RNA design and the effects of fixation on chromatin–protein interactions, CRISPR-based imaging holds great promise for advancing our understanding of chromatin dynamics, genomic interactions and their roles in various biological processes. |
| format | Article |
| id | doaj-art-fa91b3958de34f6999c259675087d97f |
| institution | Kabale University |
| issn | 2092-6413 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Experimental and Molecular Medicine |
| spelling | doaj-art-fa91b3958de34f6999c259675087d97f2025-08-20T04:01:47ZengNature Publishing GroupExperimental and Molecular Medicine2092-64132025-07-015771400140810.1038/s12276-025-01434-zUnveiling the invisible genomic dynamicsJiwoong Kwon0Yeji Park1Taekjip Ha2Program in Cellular and Molecular Medicine, Boston Children’s HospitalProgram in Cellular and Molecular Medicine, Boston Children’s HospitalProgram in Cellular and Molecular Medicine, Boston Children’s HospitalAbstract CRISPR-based imaging technologies have emerged as powerful tools for visualizing specific genomic loci, providing groundbreaking insights into chromatin structure and dynamics. Here, in this Review, we discuss the development and recent advances in these techniques, highlighting key strategies such as signal amplification, background reduction, multiplexing and enhanced genomic resolution. By engineering Cas proteins and guide RNAs, and incorporating peptide and aptamer tags, researchers have remarkably improved the sensitivity, specificity and resolution of CRISPR-based imaging, enabling the detection of nonrepetitive genomic regions and single-nucleotide polymorphisms. Recent studies have further pushed the boundaries of CRISPR-based imaging with the introduction of degron-mediated fluorogenic labeling and light-controllable background reduction. Despite remaining challenges, such as the bulkiness of signal amplification systems, limitations in guide RNA design and the effects of fixation on chromatin–protein interactions, CRISPR-based imaging holds great promise for advancing our understanding of chromatin dynamics, genomic interactions and their roles in various biological processes.https://doi.org/10.1038/s12276-025-01434-z |
| spellingShingle | Jiwoong Kwon Yeji Park Taekjip Ha Unveiling the invisible genomic dynamics Experimental and Molecular Medicine |
| title | Unveiling the invisible genomic dynamics |
| title_full | Unveiling the invisible genomic dynamics |
| title_fullStr | Unveiling the invisible genomic dynamics |
| title_full_unstemmed | Unveiling the invisible genomic dynamics |
| title_short | Unveiling the invisible genomic dynamics |
| title_sort | unveiling the invisible genomic dynamics |
| url | https://doi.org/10.1038/s12276-025-01434-z |
| work_keys_str_mv | AT jiwoongkwon unveilingtheinvisiblegenomicdynamics AT yejipark unveilingtheinvisiblegenomicdynamics AT taekjipha unveilingtheinvisiblegenomicdynamics |