Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage
Liangxue Tongyu Prescription (LTP) is a classic herbal formula for treating acute intracerebral hemorrhage (AICH) in China. Previous studies have shown that LTP significantly ameliorates neurological impairments and gastrointestinal dysfunction in patients with AICH. However, the underlying molecula...
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Elsevier
2025-01-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923024003204 |
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| author | Yingying Sun Dandan Zhou Anlan Liu Yu Zhou Yang Zhao Yuan Yuan Weifeng Guo Jianxiang Li |
| author_facet | Yingying Sun Dandan Zhou Anlan Liu Yu Zhou Yang Zhao Yuan Yuan Weifeng Guo Jianxiang Li |
| author_sort | Yingying Sun |
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| description | Liangxue Tongyu Prescription (LTP) is a classic herbal formula for treating acute intracerebral hemorrhage (AICH) in China. Previous studies have shown that LTP significantly ameliorates neurological impairments and gastrointestinal dysfunction in patients with AICH. However, the underlying molecular mechanism remains unclear. The aim of this study is to investigate whether LTP exerts its neuroprotective effect on AICH rats through the microbiota-gut-brain axis and explore its potential underlying mechanism. In the current study, AICH models were established by injecting autologous whole blood into the right caudate nucleus of rats. Behavioural and pathological evaluations demonstrated that LTP ameliorated neuronal and intestinal damage in AICH rats. Analysis via western blot, quantitative real-time PCR, immunohistochemistry (IHC) and tunel staining indicated that LTP upregulated the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor(NGF) and reduced neuronal cell apoptosis. Additionally, 16S rDNA sequencing revealed that LTP mitigated dysbiosis of intestinal microbiota in AICH rats. LTP increased the levels of noradrenaline (NA), dopamine (DA), glutamate (GLU) and modulated brain-gut peptides such as gastrin (GAS), motilin (MTL), ghrelin in AICH rats. Furthermore, LTP enhanced vagus nerve discharge. In summary, this research provides evidence suggesting that LTP’s influence on AICH may involve modulation of the microbiota-gut-brain axis, offering a potential scientific rationale for its therapeutic efficacy in improving outcomes of AICH. |
| format | Article |
| id | doaj-art-fa8a43162a814a738c966ab2d1330ebb |
| institution | Kabale University |
| issn | 1873-2747 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
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| series | Brain Research Bulletin |
| spelling | doaj-art-fa8a43162a814a738c966ab2d1330ebb2025-01-10T04:37:06ZengElsevierBrain Research Bulletin1873-27472025-01-01220111186Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhageYingying Sun0Dandan Zhou1Anlan Liu2Yu Zhou3Yang Zhao4Yuan Yuan5Weifeng Guo6Jianxiang Li7First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaFirst School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaFirst School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaFirst School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaFirst School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, ChinaFirst School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Correspondence to: School of Chinese Medicine, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Qixia District, Nanjing, Jiangsu, China.First School of Clinical Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Correspondence to: School of Chinese Medicine, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Qixia District, Nanjing, Jiangsu, China.School of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210023, China; Department of Neurology, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210022, China; Correspondence to: School of Chinese Medicine, Nanjing University of Chinese Medicine, No. 138 Xianlin Avenue, Qixia District, Nanjing, Jiangsu, China.Liangxue Tongyu Prescription (LTP) is a classic herbal formula for treating acute intracerebral hemorrhage (AICH) in China. Previous studies have shown that LTP significantly ameliorates neurological impairments and gastrointestinal dysfunction in patients with AICH. However, the underlying molecular mechanism remains unclear. The aim of this study is to investigate whether LTP exerts its neuroprotective effect on AICH rats through the microbiota-gut-brain axis and explore its potential underlying mechanism. In the current study, AICH models were established by injecting autologous whole blood into the right caudate nucleus of rats. Behavioural and pathological evaluations demonstrated that LTP ameliorated neuronal and intestinal damage in AICH rats. Analysis via western blot, quantitative real-time PCR, immunohistochemistry (IHC) and tunel staining indicated that LTP upregulated the expression of brain-derived neurotrophic factor (BDNF) and nerve growth factor(NGF) and reduced neuronal cell apoptosis. Additionally, 16S rDNA sequencing revealed that LTP mitigated dysbiosis of intestinal microbiota in AICH rats. LTP increased the levels of noradrenaline (NA), dopamine (DA), glutamate (GLU) and modulated brain-gut peptides such as gastrin (GAS), motilin (MTL), ghrelin in AICH rats. Furthermore, LTP enhanced vagus nerve discharge. In summary, this research provides evidence suggesting that LTP’s influence on AICH may involve modulation of the microbiota-gut-brain axis, offering a potential scientific rationale for its therapeutic efficacy in improving outcomes of AICH.http://www.sciencedirect.com/science/article/pii/S0361923024003204Microbiota-gut-brain axisBrain-gut peptidesNeuroprotectionLiangxue Tongyu prescriptionAcute intracerebral hemorrhage |
| spellingShingle | Yingying Sun Dandan Zhou Anlan Liu Yu Zhou Yang Zhao Yuan Yuan Weifeng Guo Jianxiang Li Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage Brain Research Bulletin Microbiota-gut-brain axis Brain-gut peptides Neuroprotection Liangxue Tongyu prescription Acute intracerebral hemorrhage |
| title | Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage |
| title_full | Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage |
| title_fullStr | Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage |
| title_full_unstemmed | Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage |
| title_short | Liangxue Tongyu Prescription exerts neuroprotection by regulating the microbiota-gut-brain axis of rats with acute intracerebral hemorrhage |
| title_sort | liangxue tongyu prescription exerts neuroprotection by regulating the microbiota gut brain axis of rats with acute intracerebral hemorrhage |
| topic | Microbiota-gut-brain axis Brain-gut peptides Neuroprotection Liangxue Tongyu prescription Acute intracerebral hemorrhage |
| url | http://www.sciencedirect.com/science/article/pii/S0361923024003204 |
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