A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates
Abstract The recent worldwide outbreaks of mpox prioritize the development of a safe and effective mRNA vaccine. The contemporary mpox virus (MPXV) exhibits changing virological and epidemiological features, notably affecting populations already vulnerable to human immunodeficiency virus (HIV). Here...
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-54909-4 |
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| author | Qing Ye Dong Zhang Rong-Rong Zhang Qian Xu Xing-Yao Huang Baoying Huang Meng-Xu Sun Zhe Cong Lin Zhu Jianrong Ma Na Li Jingjing Zhang Ting Chen Jiahan Lu Yongzhi Hou Xiang Chen Hai-Tao Liu Chao Zhou Rui-Ting Li Mei Wu Zheng-Jian Wang Jiye Yin Ye-Feng Qiu Bo Ying Wen-Jie Tan Jing Xue Cheng-Feng Qin |
| author_facet | Qing Ye Dong Zhang Rong-Rong Zhang Qian Xu Xing-Yao Huang Baoying Huang Meng-Xu Sun Zhe Cong Lin Zhu Jianrong Ma Na Li Jingjing Zhang Ting Chen Jiahan Lu Yongzhi Hou Xiang Chen Hai-Tao Liu Chao Zhou Rui-Ting Li Mei Wu Zheng-Jian Wang Jiye Yin Ye-Feng Qiu Bo Ying Wen-Jie Tan Jing Xue Cheng-Feng Qin |
| author_sort | Qing Ye |
| collection | DOAJ |
| description | Abstract The recent worldwide outbreaks of mpox prioritize the development of a safe and effective mRNA vaccine. The contemporary mpox virus (MPXV) exhibits changing virological and epidemiological features, notably affecting populations already vulnerable to human immunodeficiency virus (HIV). Herein, we profile the immunogenicity of AR-MPXV5, a penta-component mRNA vaccine targeting five specific proteins (M1R, E8L, A29L, A35R, and B6R) from the representative contemporary MPXV clade II strain, in both naive and simian immunodeficiency virus (SIV)-infected nonhuman primates. Immunization with two doses of AR-MPXV5 to cynomolgus macaques effectively elicits antibody responses and cellular responses. Importantly, based on the challenge model with a contemporary MPXV clade II strain, AR-MPXV5 demonstrates effective efficacy in preventing skin lesions, eliminating viremia and reducing viral loads in multiple tissues after challenge in naive male animals. More importantly, AR-MPXV5 is well-tolerated in stable chronic SIV-infected rhesus monkeys, while eliciting comparable MPXV-specific humoral and cellular responses in both naive and SIV-infected monkeys. Together, these results support further clinical development of the AR-MPXV5 vaccine. |
| format | Article |
| id | doaj-art-fa66c86426ab4fd5b11a076ab4cc63e1 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-fa66c86426ab4fd5b11a076ab4cc63e12024-12-08T12:35:07ZengNature PortfolioNature Communications2041-17232024-12-0115111410.1038/s41467-024-54909-4A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primatesQing Ye0Dong Zhang1Rong-Rong Zhang2Qian Xu3Xing-Yao Huang4Baoying Huang5Meng-Xu Sun6Zhe Cong7Lin Zhu8Jianrong Ma9Na Li10Jingjing Zhang11Ting Chen12Jiahan Lu13Yongzhi Hou14Xiang Chen15Hai-Tao Liu16Chao Zhou17Rui-Ting Li18Mei Wu19Zheng-Jian Wang20Jiye Yin21Ye-Feng Qiu22Bo Ying23Wen-Jie Tan24Jing Xue25Cheng-Feng Qin26State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesSchool of Basic Medical Sciences, Wenzhou Medical UniversityState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesNHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesNational Beijing Center for Drug Safety Evaluation and Research, Beijing Institute of Pharmacology and ToxicologyLaboratory Animal Center, Academy of Military Medical ScienceSuzhou Abogen Biosciences Co., Ltd.NHC Key Laboratory of Biosafety, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and PreventionState Key Laboratory of Respiratory Health and Multimorbidity, Beijing Key Laboratory for Animal Models of Emerging and Remerging Infectious Diseases, Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical CollegeState Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical SciencesAbstract The recent worldwide outbreaks of mpox prioritize the development of a safe and effective mRNA vaccine. The contemporary mpox virus (MPXV) exhibits changing virological and epidemiological features, notably affecting populations already vulnerable to human immunodeficiency virus (HIV). Herein, we profile the immunogenicity of AR-MPXV5, a penta-component mRNA vaccine targeting five specific proteins (M1R, E8L, A29L, A35R, and B6R) from the representative contemporary MPXV clade II strain, in both naive and simian immunodeficiency virus (SIV)-infected nonhuman primates. Immunization with two doses of AR-MPXV5 to cynomolgus macaques effectively elicits antibody responses and cellular responses. Importantly, based on the challenge model with a contemporary MPXV clade II strain, AR-MPXV5 demonstrates effective efficacy in preventing skin lesions, eliminating viremia and reducing viral loads in multiple tissues after challenge in naive male animals. More importantly, AR-MPXV5 is well-tolerated in stable chronic SIV-infected rhesus monkeys, while eliciting comparable MPXV-specific humoral and cellular responses in both naive and SIV-infected monkeys. Together, these results support further clinical development of the AR-MPXV5 vaccine.https://doi.org/10.1038/s41467-024-54909-4 |
| spellingShingle | Qing Ye Dong Zhang Rong-Rong Zhang Qian Xu Xing-Yao Huang Baoying Huang Meng-Xu Sun Zhe Cong Lin Zhu Jianrong Ma Na Li Jingjing Zhang Ting Chen Jiahan Lu Yongzhi Hou Xiang Chen Hai-Tao Liu Chao Zhou Rui-Ting Li Mei Wu Zheng-Jian Wang Jiye Yin Ye-Feng Qiu Bo Ying Wen-Jie Tan Jing Xue Cheng-Feng Qin A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates Nature Communications |
| title | A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates |
| title_full | A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates |
| title_fullStr | A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates |
| title_full_unstemmed | A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates |
| title_short | A penta-component mpox mRNA vaccine induces protective immunity in nonhuman primates |
| title_sort | penta component mpox mrna vaccine induces protective immunity in nonhuman primates |
| url | https://doi.org/10.1038/s41467-024-54909-4 |
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