Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus
<p class="IsiAbstrakIndo"><span lang="EN-GB">Okra (</span><em><span lang="EN-GB">Abelmoschus esculentus</span></em><span lang="EN-GB">) is reported to have various biological functions such as antioxidant, antica...
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Universitas Negeri Semarang
2017-12-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11284 |
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| author | Sri Puji Astuti Wahyuningsih Manikya Pramudya Intan Permata Putri Nadyatul Ilma Indah Savira Dwi Winarni Listijani Suhargo Win Darmanto |
| author_facet | Sri Puji Astuti Wahyuningsih Manikya Pramudya Intan Permata Putri Nadyatul Ilma Indah Savira Dwi Winarni Listijani Suhargo Win Darmanto |
| author_sort | Sri Puji Astuti Wahyuningsih |
| collection | DOAJ |
| description | <p class="IsiAbstrakIndo"><span lang="EN-GB">Okra (</span><em><span lang="EN-GB">Abelmoschus esculentus</span></em><span lang="EN-GB">) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by </span><em><span lang="EN-GB">Staphylococcus aureus</span></em><span lang="EN-GB">. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without </span><em><span lang="EN-GB">Staphylococcus aureus</span></em><span lang="EN-GB"> infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.</span></p> |
| format | Article |
| id | doaj-art-fa3d6849e7b64db38e54b0e081772b01 |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Universitas Negeri Semarang |
| record_format | Article |
| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-fa3d6849e7b64db38e54b0e081772b012025-08-20T03:20:03ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102017-12-019346046510.15294/biosaintifika.v9i3.112846533Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureusSri Puji Astuti Wahyuningsih0Manikya Pramudya1Intan Permata Putri2Nadyatul Ilma Indah Savira3Dwi Winarni4Listijani Suhargo5Win Darmanto6Department of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas AirlanggaDepartment of Biology, Faculty of Science and Technology, Universitas Airlangga<p class="IsiAbstrakIndo"><span lang="EN-GB">Okra (</span><em><span lang="EN-GB">Abelmoschus esculentus</span></em><span lang="EN-GB">) is reported to have various biological functions such as antioxidant, anticancer and anti-inflammation. However, only few studies have been reported immunomodulatory activities of okra to prevent disease caused by bacteria infection. In this study, the immunomodulatory activities of polysaccharides from okra pods were investigated further through the spleen weight and B-lymphocytes proliferation in mice infected by </span><em><span lang="EN-GB">Staphylococcus aureus</span></em><span lang="EN-GB">. Okra polysaccharides were obtained by water extraction and ethanol precipitation. Okra polysaccharides with doses of 25, 50, 75, and 100 mg/kg BW were orally administrated to mice with or without </span><em><span lang="EN-GB">Staphylococcus aureus</span></em><span lang="EN-GB"> infection. Spleen weight was evaluated in both treatment group and control group. B-lymphocytes proliferation was evaluated by MTT assay using LPS induction. Results showed that okra polysaccharide at the doses of 50, 75, and 100 mg/kg increased spleen weight (p < 0.05) significantly. While at the dose of 75 and 100 mg/kg, it increased the B-lymphocytes proliferation (p < 0.05) significantly. There is positive correlation between the spleen weight and B-lymphocytes proliferation by 73.3%. These result reveal that okra polysaccharide could improve the immune response and be utilized as a novel candidate of neutraceutical.</span></p>https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11284B-lymphocytes proliferationImmunomodulatory activityOkra polysaccharidesSpleen weight |
| spellingShingle | Sri Puji Astuti Wahyuningsih Manikya Pramudya Intan Permata Putri Nadyatul Ilma Indah Savira Dwi Winarni Listijani Suhargo Win Darmanto Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus Biosaintifika: Journal of Biology & Biology Education B-lymphocytes proliferation Immunomodulatory activity Okra polysaccharides Spleen weight |
| title | Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus |
| title_full | Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus |
| title_fullStr | Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus |
| title_full_unstemmed | Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus |
| title_short | Okra Polysaccharides Improves Spleen Weight and B-Lymphocytes Proliferation in Mice Infected by Staphylococcus aureus |
| title_sort | okra polysaccharides improves spleen weight and b lymphocytes proliferation in mice infected by staphylococcus aureus |
| topic | B-lymphocytes proliferation Immunomodulatory activity Okra polysaccharides Spleen weight |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/11284 |
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