Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study
Introduction: The aim of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) in Algerian hospitals and to characterize the molecular types of carbapenemases found. Methodology: During a four years study lasting between 2012 and 2015, 81 strains of Enter...
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The Journal of Infection in Developing Countries
2018-11-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/9692 |
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| author | Nadjet Aggoune Hassiba Tali Maamar Farida Assaous Badia Guettou Rym Laliam Nabila Benamrouche Ali Zerouki Malek Naim Kheira Rahal |
| author_facet | Nadjet Aggoune Hassiba Tali Maamar Farida Assaous Badia Guettou Rym Laliam Nabila Benamrouche Ali Zerouki Malek Naim Kheira Rahal |
| author_sort | Nadjet Aggoune |
| collection | DOAJ |
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Introduction: The aim of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) in Algerian hospitals and to characterize the molecular types of carbapenemases found.
Methodology: During a four years study lasting between 2012 and 2015, 81 strains of Enterobacteriaceae with reduced susceptibility to carbapenems were collected from different hospitals. Carbapenemase genes were detected by PCR. Multi locus sequence typing was used to study genetic relationships between carbapenemase- producing Klebsiella pneumoniae isolates.
Results: Among 56 confirmed CPE, blaOXA-48 was detected in 98.21% of isolates. Two isolates co-expressed NDM, and a single one was only an NDM producer. The strains displayed various susceptibility patterns to antibiotics with variable levels of resistance to carbapenems. Multilocus sequence typing (MLST) revealed the presence of multiple sequence types in circulation.
Conclusions: This report highlights the wide distribution of several clones of OXA-48-producing Enterobacteriaceae in Algeria. Urgent action should be taken to avoid epidemic situations.
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| format | Article |
| id | doaj-art-fa36d4a1e3f24bd69f29d2bc2917dad1 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-fa36d4a1e3f24bd69f29d2bc2917dad12025-08-20T03:48:46ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802018-11-01121110.3855/jidc.9692Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ studyNadjet Aggoune0Hassiba Tali Maamar1Farida Assaous2Badia Guettou3Rym Laliam4Nabila Benamrouche5Ali Zerouki6Malek Naim7Kheira Rahal8Hôpital central de l’armée Mohammed Seghir Nekkache, Alger, AlgérieInstitut Pasteur d’Algérie Alger, AlgérieInstitut Pasteur d’Algérie Alger, AlgérieInstitut Pasteur d’Algérie Alger, AlgérieInstitut Pasteur d’Algérie Alger, AlgérieInstitut Pasteur d’Algérie Alger, AlgérieHôpital central de l’armée Mohammed Seghir Nekkache, Alger, AlgérieHôpital central de l’armée Mohammed Seghir Nekkache, Alger, AlgérieInstitut Pasteur d’Algérie Alger, Algérie Introduction: The aim of this study was to investigate the presence of carbapenemase-producing Enterobacteriaceae (CPE) in Algerian hospitals and to characterize the molecular types of carbapenemases found. Methodology: During a four years study lasting between 2012 and 2015, 81 strains of Enterobacteriaceae with reduced susceptibility to carbapenems were collected from different hospitals. Carbapenemase genes were detected by PCR. Multi locus sequence typing was used to study genetic relationships between carbapenemase- producing Klebsiella pneumoniae isolates. Results: Among 56 confirmed CPE, blaOXA-48 was detected in 98.21% of isolates. Two isolates co-expressed NDM, and a single one was only an NDM producer. The strains displayed various susceptibility patterns to antibiotics with variable levels of resistance to carbapenems. Multilocus sequence typing (MLST) revealed the presence of multiple sequence types in circulation. Conclusions: This report highlights the wide distribution of several clones of OXA-48-producing Enterobacteriaceae in Algeria. Urgent action should be taken to avoid epidemic situations. https://jidc.org/index.php/journal/article/view/9692CarbapenemaseOXA-48NDMKlebsiella pneumoniaesequence typeAlgeria |
| spellingShingle | Nadjet Aggoune Hassiba Tali Maamar Farida Assaous Badia Guettou Rym Laliam Nabila Benamrouche Ali Zerouki Malek Naim Kheira Rahal Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study Journal of Infection in Developing Countries Carbapenemase OXA-48 NDM Klebsiella pneumoniae sequence type Algeria |
| title | Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study |
| title_full | Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study |
| title_fullStr | Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study |
| title_full_unstemmed | Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study |
| title_short | Wide spread of OXA-48-producing Enterobacteriaceae in Algerian hospitals: A four years’ study |
| title_sort | wide spread of oxa 48 producing enterobacteriaceae in algerian hospitals a four years study |
| topic | Carbapenemase OXA-48 NDM Klebsiella pneumoniae sequence type Algeria |
| url | https://jidc.org/index.php/journal/article/view/9692 |
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