Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium
Abstract In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curd...
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Nature Portfolio
2024-11-01
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| Online Access: | https://doi.org/10.1038/s41598-024-77880-y |
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| author | Natasha Aquinas Bhat M. Ramananda Subbalaxmi Selvaraj |
| author_facet | Natasha Aquinas Bhat M. Ramananda Subbalaxmi Selvaraj |
| author_sort | Natasha Aquinas |
| collection | DOAJ |
| description | Abstract In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curdlan yield improved from 0.15 g/L (unoptimized) to 0.46 g/L (3-fold increase) when fermentation was carried out in CCD-optimized media of (w/v) sucrose 20%, urea 0.1%, KH2PO4 0.02%, agitation speed 250 rpm. To further enhance curdlan yield during extraction, ultrasonication was incorporated as a novel step into the conventional method of acid/alkali-assisted curdlan recovery. A two-step optimization was chosen for extraction, namely, one-factor-at-a-time (OFAT) and CCD, wherein the optimized extraction parameters were determined to be 25 s sonication, 1 N NaOH, and 2 h solubilization time. The curdlan yield improved by 1.5-fold (0.70 g/L) post optimization, in comparison with unoptimized conventional extraction step. Finally, the biopolymer was validated through characterization by nuclear magnetic resonance (NMR) which showed characteristic curdlan spectra in the13C and1H NMR studies. To the best of our knowledge, this study represents the first documented report on curdlan extraction using this novel method of ultrasonication. |
| format | Article |
| id | doaj-art-fa360f9fd1ab4a22a9f86f9fbd726b33 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
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| series | Scientific Reports |
| spelling | doaj-art-fa360f9fd1ab4a22a9f86f9fbd726b332024-11-10T12:24:51ZengNature PortfolioScientific Reports2045-23222024-11-0114111210.1038/s41598-024-77880-yOptimization of curdlan production and ultrasound assisted extraction processes from Priestia megateriumNatasha Aquinas0Bhat M. Ramananda1Subbalaxmi Selvaraj2Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE)Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE)Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education (MAHE)Abstract In this study, the upstream and downstream production processes of curdlan from Priestia megaterium were optimized to enhance its yield. Additionally, a novel extraction method was developed for curdlan recovery. Optimization studies were conducted using Central composite design (CCD). Curdlan yield improved from 0.15 g/L (unoptimized) to 0.46 g/L (3-fold increase) when fermentation was carried out in CCD-optimized media of (w/v) sucrose 20%, urea 0.1%, KH2PO4 0.02%, agitation speed 250 rpm. To further enhance curdlan yield during extraction, ultrasonication was incorporated as a novel step into the conventional method of acid/alkali-assisted curdlan recovery. A two-step optimization was chosen for extraction, namely, one-factor-at-a-time (OFAT) and CCD, wherein the optimized extraction parameters were determined to be 25 s sonication, 1 N NaOH, and 2 h solubilization time. The curdlan yield improved by 1.5-fold (0.70 g/L) post optimization, in comparison with unoptimized conventional extraction step. Finally, the biopolymer was validated through characterization by nuclear magnetic resonance (NMR) which showed characteristic curdlan spectra in the13C and1H NMR studies. To the best of our knowledge, this study represents the first documented report on curdlan extraction using this novel method of ultrasonication.https://doi.org/10.1038/s41598-024-77880-yCurdlanOptimizationExtractionUltrasonicationPriestia megaterium |
| spellingShingle | Natasha Aquinas Bhat M. Ramananda Subbalaxmi Selvaraj Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium Scientific Reports Curdlan Optimization Extraction Ultrasonication Priestia megaterium |
| title | Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium |
| title_full | Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium |
| title_fullStr | Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium |
| title_full_unstemmed | Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium |
| title_short | Optimization of curdlan production and ultrasound assisted extraction processes from Priestia megaterium |
| title_sort | optimization of curdlan production and ultrasound assisted extraction processes from priestia megaterium |
| topic | Curdlan Optimization Extraction Ultrasonication Priestia megaterium |
| url | https://doi.org/10.1038/s41598-024-77880-y |
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