The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins
<b>Aim: </b>Spontaneous capsular loss or capsular changes in Pasteurella multocida can result from repeated laboratory passages, positive or negative regulatory genes, or an unknown gene. This study, it was aimed to compare the properties of phenotypic, genotypic, and biotypic of each no...
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| Language: | English |
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Selcuk University Press
2023-09-01
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| Series: | Eurasian Journal of Veterinary Sciences |
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| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1430 |
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| author | Asli Balevi Aysegul Ilban Ali Uslu Zafer Sayin Ayten Gok Beatriz Padron Eda Toslak Osman Erganis |
| author_facet | Asli Balevi Aysegul Ilban Ali Uslu Zafer Sayin Ayten Gok Beatriz Padron Eda Toslak Osman Erganis |
| author_sort | Asli Balevi |
| collection | DOAJ |
| description | <b>Aim: </b>Spontaneous capsular loss or capsular changes in Pasteurella multocida
can result from repeated laboratory passages, positive or negative regulatory
genes, or an unknown gene. This study, it was aimed to compare the properties
of phenotypic, genotypic, and biotypic of each non-typical, and typical Pasteurella
multocida strain, to determine the dominant genes on capsule synthesis.<p>
<b>Materials and Methods:</b> Fifty-six strains, which capsular type was
determined, and thirty -six, which capsular type was not determined, were
used in this study. Multiple linear regression analysis was used based on
the presence of dominant genes (serogroup, serotype, toxin, adhesin, iron
acquisition, and protectin) in the isolates.<p>
<b>Results:</b> When colony morphologies of strains were evaluated of these strains
by culture methods, mucoid colony formation was commonly detected in
typical strains (87.5%), in contrast to non-typical strains (27.7%). In typical
strains, the highest percentages of ptfA, ompA, and tadD genes were 78.57%,
75%, and 69.64%, respectively. In non-typical strains, the highest rates of
ompA, ptfA, and tadD genes were 61.1%, 52.78%, and 52.78%, respectively.
According to multiple linear regression analysis results, the together hgbA
with hgbB genes caused the increase of capsule synthesis in these strains. The
presence of the ompA gene in these strains was secondly a induction on these
strains. Other genes were not effective in capsule synthesis in these strains.<p>
<b>Conclusion:</b> It was determined that the most significant effect in the forming
of non-typical P. multocida strains was related to not enough HgbA and HgbB
genes. It was supposed that P. multocida may not be heavily encapsulated
under iron-restricted conditions. Consequently, P. multocida may change its
capsule or lose its capsule related to iron acquisition proteins. |
| format | Article |
| id | doaj-art-fa2c5a64e2d14bd2a2fb473ab7dc53b5 |
| institution | Kabale University |
| issn | 1309-6958 2146-1953 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Selcuk University Press |
| record_format | Article |
| series | Eurasian Journal of Veterinary Sciences |
| spelling | doaj-art-fa2c5a64e2d14bd2a2fb473ab7dc53b52025-01-03T01:36:24ZengSelcuk University PressEurasian Journal of Veterinary Sciences1309-69582146-19532023-09-013931391431430The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteinsAsli Balevi0Aysegul Ilban1Ali Uslu2Zafer Sayin3Ayten Gok4Beatriz Padron5Eda Toslak6Osman Erganis7Selcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeDepartment of Microbiology, Konya Numune Hospital, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, TürkiyeSelcuk University, Faculty of Veterinary Medicine, Department of Microbiology, Konya, Türkiye<b>Aim: </b>Spontaneous capsular loss or capsular changes in Pasteurella multocida can result from repeated laboratory passages, positive or negative regulatory genes, or an unknown gene. This study, it was aimed to compare the properties of phenotypic, genotypic, and biotypic of each non-typical, and typical Pasteurella multocida strain, to determine the dominant genes on capsule synthesis.<p> <b>Materials and Methods:</b> Fifty-six strains, which capsular type was determined, and thirty -six, which capsular type was not determined, were used in this study. Multiple linear regression analysis was used based on the presence of dominant genes (serogroup, serotype, toxin, adhesin, iron acquisition, and protectin) in the isolates.<p> <b>Results:</b> When colony morphologies of strains were evaluated of these strains by culture methods, mucoid colony formation was commonly detected in typical strains (87.5%), in contrast to non-typical strains (27.7%). In typical strains, the highest percentages of ptfA, ompA, and tadD genes were 78.57%, 75%, and 69.64%, respectively. In non-typical strains, the highest rates of ompA, ptfA, and tadD genes were 61.1%, 52.78%, and 52.78%, respectively. According to multiple linear regression analysis results, the together hgbA with hgbB genes caused the increase of capsule synthesis in these strains. The presence of the ompA gene in these strains was secondly a induction on these strains. Other genes were not effective in capsule synthesis in these strains.<p> <b>Conclusion:</b> It was determined that the most significant effect in the forming of non-typical P. multocida strains was related to not enough HgbA and HgbB genes. It was supposed that P. multocida may not be heavily encapsulated under iron-restricted conditions. Consequently, P. multocida may change its capsule or lose its capsule related to iron acquisition proteins.http://eurasianjvetsci.org/pdf.php3?id=1430pasteurella multocidapneumoniamultiple linear regression analysisvirulence-associated genes |
| spellingShingle | Asli Balevi Aysegul Ilban Ali Uslu Zafer Sayin Ayten Gok Beatriz Padron Eda Toslak Osman Erganis The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins Eurasian Journal of Veterinary Sciences pasteurella multocida pneumonia multiple linear regression analysis virulence-associated genes |
| title | The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins |
| title_full | The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins |
| title_fullStr | The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins |
| title_full_unstemmed | The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins |
| title_short | The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins |
| title_sort | enigmatical manipulators in the capsule synthesis of pasteurella multocida iron acquisition proteins |
| topic | pasteurella multocida pneumonia multiple linear regression analysis virulence-associated genes |
| url | http://eurasianjvetsci.org/pdf.php3?id=1430 |
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