The enigmatical manipulators in the capsule synthesis of pasteurella multocida: iron acquisition proteins
<b>Aim: </b>Spontaneous capsular loss or capsular changes in Pasteurella multocida can result from repeated laboratory passages, positive or negative regulatory genes, or an unknown gene. This study, it was aimed to compare the properties of phenotypic, genotypic, and biotypic of each no...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2023-09-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1430 |
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| Summary: | <b>Aim: </b>Spontaneous capsular loss or capsular changes in Pasteurella multocida
can result from repeated laboratory passages, positive or negative regulatory
genes, or an unknown gene. This study, it was aimed to compare the properties
of phenotypic, genotypic, and biotypic of each non-typical, and typical Pasteurella
multocida strain, to determine the dominant genes on capsule synthesis.<p>
<b>Materials and Methods:</b> Fifty-six strains, which capsular type was
determined, and thirty -six, which capsular type was not determined, were
used in this study. Multiple linear regression analysis was used based on
the presence of dominant genes (serogroup, serotype, toxin, adhesin, iron
acquisition, and protectin) in the isolates.<p>
<b>Results:</b> When colony morphologies of strains were evaluated of these strains
by culture methods, mucoid colony formation was commonly detected in
typical strains (87.5%), in contrast to non-typical strains (27.7%). In typical
strains, the highest percentages of ptfA, ompA, and tadD genes were 78.57%,
75%, and 69.64%, respectively. In non-typical strains, the highest rates of
ompA, ptfA, and tadD genes were 61.1%, 52.78%, and 52.78%, respectively.
According to multiple linear regression analysis results, the together hgbA
with hgbB genes caused the increase of capsule synthesis in these strains. The
presence of the ompA gene in these strains was secondly a induction on these
strains. Other genes were not effective in capsule synthesis in these strains.<p>
<b>Conclusion:</b> It was determined that the most significant effect in the forming
of non-typical P. multocida strains was related to not enough HgbA and HgbB
genes. It was supposed that P. multocida may not be heavily encapsulated
under iron-restricted conditions. Consequently, P. multocida may change its
capsule or lose its capsule related to iron acquisition proteins. |
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| ISSN: | 1309-6958 2146-1953 |