Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches
Roses (Rosa chinensis) are among the most cherished ornamental plants globally, yet they are highly susceptible to infections by Botrytis cinerea, the causative agent of gray mold disease. Here we inoculated the resistant rose variety ‘Yellow Leisure Liness’ with B. cinerea to investigate its resist...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2024.1511597/full |
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| author | Haoyuan Chen Qingkui Li Peilei Cheng Taotao Yan Chunlan Dong Zhe Hou Peihuang Zhu Changbing Huang |
| author_facet | Haoyuan Chen Qingkui Li Peilei Cheng Taotao Yan Chunlan Dong Zhe Hou Peihuang Zhu Changbing Huang |
| author_sort | Haoyuan Chen |
| collection | DOAJ |
| description | Roses (Rosa chinensis) are among the most cherished ornamental plants globally, yet they are highly susceptible to infections by Botrytis cinerea, the causative agent of gray mold disease. Here we inoculated the resistant rose variety ‘Yellow Leisure Liness’ with B. cinerea to investigate its resistance mechanisms against gray mold disease. Through transcriptome sequencing, we identified 578 differentially expressed genes (DEGs) that were significantly upregulated at 24, 48, and 72 hours post-inoculation, with these genes significantly enriched for three defense response-related GO terms. Further domain analysis of the genes in these GO terms reveal that 21 DEGs contain the Bet v 1 family domain, belonging to the major latex protein (MLP) gene family, suggesting their potential key role in rose disease resistance. Furthermore, we systematically identified 46 RcMLP genes in roses and phylogenetically categorized them into two distinct subfamilies: group I and II. Genomic duplication analysis indicates that tandem duplication is the main driver for the expansion of the RcMLP family, and these genes have undergone by purifying selection. Additionally, detailed analyses of gene structure, motif composition, and promoter regions reveal that RcMLP genes contain numerous stress-responsive elements, with 32 RcMLP genes harboring fungal elicitor/wound-responsive elements. The constructed potential transcription factor regulatory network showed significant enrichment of the ERF transcription factor family in the regulation of RcMLP genes. Gene expression analysis reveal that DEGs are mainly distributed in subfamily II, where four highly expressed genes (RcMLP13, RcMLP28, RcMLP14, and RcMLP27) are identified in a small branch, with their fold change exceeding ten folds and verified by qRT-PCR. In summary, our research results underscore the potential importance of the RcMLP gene family in response to B. cinerea infection and provide comprehensive basis for further function exploration of the MLP gene family in rose resistance to fungal infections. |
| format | Article |
| id | doaj-art-fa0e88cde70c4d78b2511bb656a4cc0b |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj-art-fa0e88cde70c4d78b2511bb656a4cc0b2024-12-13T04:17:15ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-12-011510.3389/fpls.2024.15115971511597Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approachesHaoyuan Chen0Qingkui Li1Peilei Cheng2Taotao Yan3Chunlan Dong4Zhe Hou5Peihuang Zhu6Changbing Huang7College of Horticultural Science and Technology, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Horticultural Science and Technology, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Landscape Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Landscape Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Horticultural Science and Technology, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Landscape Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Landscape Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaCollege of Landscape Engineering, Suzhou Polytechnic Institute of Agriculture, Suzhou, ChinaRoses (Rosa chinensis) are among the most cherished ornamental plants globally, yet they are highly susceptible to infections by Botrytis cinerea, the causative agent of gray mold disease. Here we inoculated the resistant rose variety ‘Yellow Leisure Liness’ with B. cinerea to investigate its resistance mechanisms against gray mold disease. Through transcriptome sequencing, we identified 578 differentially expressed genes (DEGs) that were significantly upregulated at 24, 48, and 72 hours post-inoculation, with these genes significantly enriched for three defense response-related GO terms. Further domain analysis of the genes in these GO terms reveal that 21 DEGs contain the Bet v 1 family domain, belonging to the major latex protein (MLP) gene family, suggesting their potential key role in rose disease resistance. Furthermore, we systematically identified 46 RcMLP genes in roses and phylogenetically categorized them into two distinct subfamilies: group I and II. Genomic duplication analysis indicates that tandem duplication is the main driver for the expansion of the RcMLP family, and these genes have undergone by purifying selection. Additionally, detailed analyses of gene structure, motif composition, and promoter regions reveal that RcMLP genes contain numerous stress-responsive elements, with 32 RcMLP genes harboring fungal elicitor/wound-responsive elements. The constructed potential transcription factor regulatory network showed significant enrichment of the ERF transcription factor family in the regulation of RcMLP genes. Gene expression analysis reveal that DEGs are mainly distributed in subfamily II, where four highly expressed genes (RcMLP13, RcMLP28, RcMLP14, and RcMLP27) are identified in a small branch, with their fold change exceeding ten folds and verified by qRT-PCR. In summary, our research results underscore the potential importance of the RcMLP gene family in response to B. cinerea infection and provide comprehensive basis for further function exploration of the MLP gene family in rose resistance to fungal infections.https://www.frontiersin.org/articles/10.3389/fpls.2024.1511597/fullmajor latex proteinroseRNA-Seqtandem duplicationBotrytis cinerea |
| spellingShingle | Haoyuan Chen Qingkui Li Peilei Cheng Taotao Yan Chunlan Dong Zhe Hou Peihuang Zhu Changbing Huang Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches Frontiers in Plant Science major latex protein rose RNA-Seq tandem duplication Botrytis cinerea |
| title | Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches |
| title_full | Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches |
| title_fullStr | Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches |
| title_full_unstemmed | Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches |
| title_short | Identification and analysis of major latex protein (MLP) family genes in Rosa chinensis responsive to Botrytis cinerea infection by RNA-seq approaches |
| title_sort | identification and analysis of major latex protein mlp family genes in rosa chinensis responsive to botrytis cinerea infection by rna seq approaches |
| topic | major latex protein rose RNA-Seq tandem duplication Botrytis cinerea |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2024.1511597/full |
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