Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study
Objectives: With the rising number of cases of oral submucous fibrosis (OSMF) undergoing malignant transformation into oral squamous cell carcinoma (OSCC), it is needed to detect the changes at the earliest. Several molecular markers have been used for this purpose, and insulin-like growth factor re...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Oral Oncology Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906024001304 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841553787362213888 |
|---|---|
| author | Vivek Nayyar Vinay Hazarey Sindhu M. Ganvir Hemant J. Purohit Reshma Talkal Kiran Jot |
| author_facet | Vivek Nayyar Vinay Hazarey Sindhu M. Ganvir Hemant J. Purohit Reshma Talkal Kiran Jot |
| author_sort | Vivek Nayyar |
| collection | DOAJ |
| description | Objectives: With the rising number of cases of oral submucous fibrosis (OSMF) undergoing malignant transformation into oral squamous cell carcinoma (OSCC), it is needed to detect the changes at the earliest. Several molecular markers have been used for this purpose, and insulin-like growth factor receptor 1 (IGF-1R) is one of the markers in vogue for several human malignancies. Thus, we compared the expression of IGF-1R mRNA in normal oral mucosa (NOM), OSMF, and OSCC. Materials and methods: Thirty biopsy tissue samples for each of the three groups, NOM, OSMF, and OSCC, were taken and analyzed histopathologically and then subjected to RT-PCR for evaluation of the expression of IGF-1R mRNA in each of them. Results: There was an upregulation of IGF-1R mRNA in OSMF tissue samples as compared to NOM, but a downregulation as compared to OSCC. There was a 3.24-fold upregulation of IGF-1R mRNA expression in OSMF as compared to NOM and a 1.31-fold downregulation as compared to OSCC. Likewise, there was a 4.2-fold upregulation of IGF-1R mRNA expression in OSCC as compared to NOM and 1.31-fold upregulation as compared to OSMF. Moreover, the expression was more in OSMF patients with interincisal opening (IIO) < 27 mm as compared to those with IIO >27 mm and also was found to increase with the grade of OSCC. Conclusion: The study reflected a potential role of IGF-1R in the pathogenesis of OSMF and OSCC, thus implying its possible exploitation as a diagnostic and prognostic marker and also as a therapeutic target in future. |
| format | Article |
| id | doaj-art-fa0cda74076d4e0ab9b2c20ada220501 |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-fa0cda74076d4e0ab9b2c20ada2205012025-01-09T06:15:39ZengElsevierOral Oncology Reports2772-90602024-06-0110100284Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR studyVivek Nayyar0Vinay Hazarey1Sindhu M. Ganvir2Hemant J. Purohit3Reshma Talkal4Kiran Jot5Centre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, India; Corresponding author.Datta Meghe Institute of Medical Sciences, Wardha, Nagpur, Maharashtra, 442004, IndiaDeptt. of Oral Pathology and Microbiology, Govt Dental College, Nagpur, Maharashtra, 440003, IndiaEnvironmental Biotechnology & Genomics Division, National Environmental Engineering Research Institute, (NEERI), CSIR, Nehru Marg, Nagpur, 440 020, IndiaGujarat Biotechnology Research Center, Gujarat, 382011, IndiaCentre for Dental Education and Research, All India Institute of Medical Sciences, New Delhi, IndiaObjectives: With the rising number of cases of oral submucous fibrosis (OSMF) undergoing malignant transformation into oral squamous cell carcinoma (OSCC), it is needed to detect the changes at the earliest. Several molecular markers have been used for this purpose, and insulin-like growth factor receptor 1 (IGF-1R) is one of the markers in vogue for several human malignancies. Thus, we compared the expression of IGF-1R mRNA in normal oral mucosa (NOM), OSMF, and OSCC. Materials and methods: Thirty biopsy tissue samples for each of the three groups, NOM, OSMF, and OSCC, were taken and analyzed histopathologically and then subjected to RT-PCR for evaluation of the expression of IGF-1R mRNA in each of them. Results: There was an upregulation of IGF-1R mRNA in OSMF tissue samples as compared to NOM, but a downregulation as compared to OSCC. There was a 3.24-fold upregulation of IGF-1R mRNA expression in OSMF as compared to NOM and a 1.31-fold downregulation as compared to OSCC. Likewise, there was a 4.2-fold upregulation of IGF-1R mRNA expression in OSCC as compared to NOM and 1.31-fold upregulation as compared to OSMF. Moreover, the expression was more in OSMF patients with interincisal opening (IIO) < 27 mm as compared to those with IIO >27 mm and also was found to increase with the grade of OSCC. Conclusion: The study reflected a potential role of IGF-1R in the pathogenesis of OSMF and OSCC, thus implying its possible exploitation as a diagnostic and prognostic marker and also as a therapeutic target in future.http://www.sciencedirect.com/science/article/pii/S2772906024001304IGF-1ROral submucous fibrosisOral squamous cell carcinoma |
| spellingShingle | Vivek Nayyar Vinay Hazarey Sindhu M. Ganvir Hemant J. Purohit Reshma Talkal Kiran Jot Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study Oral Oncology Reports IGF-1R Oral submucous fibrosis Oral squamous cell carcinoma |
| title | Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study |
| title_full | Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study |
| title_fullStr | Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study |
| title_full_unstemmed | Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study |
| title_short | Expression of IGF-1R in oral submucous fibrosis and oral squamous cell carcinoma – An RT-PCR study |
| title_sort | expression of igf 1r in oral submucous fibrosis and oral squamous cell carcinoma an rt pcr study |
| topic | IGF-1R Oral submucous fibrosis Oral squamous cell carcinoma |
| url | http://www.sciencedirect.com/science/article/pii/S2772906024001304 |
| work_keys_str_mv | AT viveknayyar expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy AT vinayhazarey expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy AT sindhumganvir expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy AT hemantjpurohit expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy AT reshmatalkal expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy AT kiranjot expressionofigf1rinoralsubmucousfibrosisandoralsquamouscellcarcinomaanrtpcrstudy |