Understanding brewing trait inheritance in de novo Lager yeast hybrids
ABSTRACT Hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus resulted in the emergence of S. pastorianus, a crucial yeast for lager fermentation. However, our understanding of hybridization success and hybrid vigor between these two species remains limited due to the scarcity...
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American Society for Microbiology
2024-12-01
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| Series: | mSystems |
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| Online Access: | https://journals.asm.org/doi/10.1128/msystems.00762-24 |
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| author | Vasni Zavaleta Laura Pérez-Través Luis A. Saona Carlos A. Villarroel Amparo Querol Francisco A. Cubillos |
| author_facet | Vasni Zavaleta Laura Pérez-Través Luis A. Saona Carlos A. Villarroel Amparo Querol Francisco A. Cubillos |
| author_sort | Vasni Zavaleta |
| collection | DOAJ |
| description | ABSTRACT Hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus resulted in the emergence of S. pastorianus, a crucial yeast for lager fermentation. However, our understanding of hybridization success and hybrid vigor between these two species remains limited due to the scarcity of S. eubayanus parental strains. Here, we explore hybridization success and the impact of hybridization on fermentation performance and volatile compound profiles in newly formed lager hybrids. By selecting parental candidates spanning a diverse array of lineages from both species, we reveal that the Beer and PB-2 lineages exhibit high rates of hybridization success in S. cerevisiae and S. eubayanus, respectively. Polyploid hybrids were generated through a spontaneous diploid hybridization technique (rare-mating), revealing a prevalence of triploids and diploids over tetraploids. Despite the absence of heterosis in fermentative capacity, hybrids displayed phenotypic variability, notably influenced by maltotriose consumption. Interestingly, ploidy levels did not significantly correlate with fermentative capacity, although triploids exhibited greater phenotypic variability. The S. cerevisiae parental lineages primarily influenced volatile compound profiles, with significant differences in aroma production. Interestingly, hybrids emerging from the Beer S. cerevisiae parental lineages exhibited a volatile compound profile resembling the corresponding S. eubayanus parent. This pattern may result from the dominant inheritance of the S. eubayanus aroma profile, as suggested by the over-expression of genes related to alcohol metabolism and acetate synthesis in hybrids including the Beer S. cerevisiae lineage. Our findings suggest complex interactions between parental lineages and hybridization outcomes, highlighting the potential for creating yeasts with distinct brewing traits through hybridization strategies.IMPORTANCEOur study investigates the principles of lager yeast hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus. This process gave rise to the lager yeast Saccharomyces pastorianus. By examining how these novel hybrids perform during fermentation and the aromas they produce, we uncover the genetic bases of brewing trait inheritance. We successfully generated polyploid hybrids using diverse strains and lineages from both parent species, predominantly triploids and diploids. Although these hybrids did not show improved fermentation capacity, they exhibited varied traits, especially in utilizing maltotriose, a key sugar in brewing. Remarkably, the aroma profiles of these hybrids were primarily influenced by the S. cerevisiae parent, with Beer lineage hybrids adopting aroma characteristics from their S. eubayanus parent. These insights reveal the complex genetic interactions in hybrid yeasts, opening new possibilities for crafting unique brewing yeasts with desirable traits. |
| format | Article |
| id | doaj-art-f9c7809ad7cc405ba056e1a1aa5606cf |
| institution | Kabale University |
| issn | 2379-5077 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | American Society for Microbiology |
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| series | mSystems |
| spelling | doaj-art-f9c7809ad7cc405ba056e1a1aa5606cf2024-12-17T14:21:13ZengAmerican Society for MicrobiologymSystems2379-50772024-12-0191210.1128/msystems.00762-24Understanding brewing trait inheritance in de novo Lager yeast hybridsVasni Zavaleta0Laura Pérez-Través1Luis A. Saona2Carlos A. Villarroel3Amparo Querol4Francisco A. Cubillos5Universidad de Santiago de Chile, Facultad de Química y Biología, Departamento de Biología, Santiago, ChileDepartamento de Biotecnología de los Alimentos, Grupo de Biología de Sistemas en Levaduras de Interés Biotecnológico, Instituto de Agroquímica y Tecnología de los Alimentos (IATA)-CSIC, Valencia, SpainUniversidad de Santiago de Chile, Facultad de Química y Biología, Departamento de Biología, Santiago, ChileMillennium Institute for Integrative Biology (iBio), Santiago, ChileDepartamento de Biotecnología de los Alimentos, Grupo de Biología de Sistemas en Levaduras de Interés Biotecnológico, Instituto de Agroquímica y Tecnología de los Alimentos (IATA)-CSIC, Valencia, SpainUniversidad de Santiago de Chile, Facultad de Química y Biología, Departamento de Biología, Santiago, ChileABSTRACT Hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus resulted in the emergence of S. pastorianus, a crucial yeast for lager fermentation. However, our understanding of hybridization success and hybrid vigor between these two species remains limited due to the scarcity of S. eubayanus parental strains. Here, we explore hybridization success and the impact of hybridization on fermentation performance and volatile compound profiles in newly formed lager hybrids. By selecting parental candidates spanning a diverse array of lineages from both species, we reveal that the Beer and PB-2 lineages exhibit high rates of hybridization success in S. cerevisiae and S. eubayanus, respectively. Polyploid hybrids were generated through a spontaneous diploid hybridization technique (rare-mating), revealing a prevalence of triploids and diploids over tetraploids. Despite the absence of heterosis in fermentative capacity, hybrids displayed phenotypic variability, notably influenced by maltotriose consumption. Interestingly, ploidy levels did not significantly correlate with fermentative capacity, although triploids exhibited greater phenotypic variability. The S. cerevisiae parental lineages primarily influenced volatile compound profiles, with significant differences in aroma production. Interestingly, hybrids emerging from the Beer S. cerevisiae parental lineages exhibited a volatile compound profile resembling the corresponding S. eubayanus parent. This pattern may result from the dominant inheritance of the S. eubayanus aroma profile, as suggested by the over-expression of genes related to alcohol metabolism and acetate synthesis in hybrids including the Beer S. cerevisiae lineage. Our findings suggest complex interactions between parental lineages and hybridization outcomes, highlighting the potential for creating yeasts with distinct brewing traits through hybridization strategies.IMPORTANCEOur study investigates the principles of lager yeast hybridization between Saccharomyces cerevisiae and Saccharomyces eubayanus. This process gave rise to the lager yeast Saccharomyces pastorianus. By examining how these novel hybrids perform during fermentation and the aromas they produce, we uncover the genetic bases of brewing trait inheritance. We successfully generated polyploid hybrids using diverse strains and lineages from both parent species, predominantly triploids and diploids. Although these hybrids did not show improved fermentation capacity, they exhibited varied traits, especially in utilizing maltotriose, a key sugar in brewing. Remarkably, the aroma profiles of these hybrids were primarily influenced by the S. cerevisiae parent, with Beer lineage hybrids adopting aroma characteristics from their S. eubayanus parent. These insights reveal the complex genetic interactions in hybrid yeasts, opening new possibilities for crafting unique brewing yeasts with desirable traits.https://journals.asm.org/doi/10.1128/msystems.00762-24yeastbeervolatile compoundslagerhybridizationRNA-seq |
| spellingShingle | Vasni Zavaleta Laura Pérez-Través Luis A. Saona Carlos A. Villarroel Amparo Querol Francisco A. Cubillos Understanding brewing trait inheritance in de novo Lager yeast hybrids mSystems yeast beer volatile compounds lager hybridization RNA-seq |
| title | Understanding brewing trait inheritance in de novo Lager yeast hybrids |
| title_full | Understanding brewing trait inheritance in de novo Lager yeast hybrids |
| title_fullStr | Understanding brewing trait inheritance in de novo Lager yeast hybrids |
| title_full_unstemmed | Understanding brewing trait inheritance in de novo Lager yeast hybrids |
| title_short | Understanding brewing trait inheritance in de novo Lager yeast hybrids |
| title_sort | understanding brewing trait inheritance in de novo lager yeast hybrids |
| topic | yeast beer volatile compounds lager hybridization RNA-seq |
| url | https://journals.asm.org/doi/10.1128/msystems.00762-24 |
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