ROS Scavenging and Osteogenic Differentiation Potential of L-Methionine-Substituted Poly(Organophosphazene) Electrospun Fibers
This study investigated the application of poly[bis (ethylmethionato) phosphazene] (PαAPz-M) electrospun fibers in tissue engineering, focusing on their reactive oxygen species (ROS) scavenging capabilities and material-directed cell behavior, including the influence of their degradation products on...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-11-01
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| Series: | Biomimetics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2313-7673/9/11/676 |
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| Summary: | This study investigated the application of poly[bis (ethylmethionato) phosphazene] (PαAPz-M) electrospun fibers in tissue engineering, focusing on their reactive oxygen species (ROS) scavenging capabilities and material-directed cell behavior, including the influence of their degradation products on cell viability and differentiation, and the scaffold topography’s influence on cell alignment. The ROS scavenging ability of PαAPz-M was assessed by DPPH assay, and then PαAPz-M’s protection against exogenous ROS was studied. The results showed enhanced cell viability on PαAPz-M fiber mats under oxidative stress conditions. This study also investigated the effects of the degradation products of PαAPz-M on cell viability and osteogenic differentiation. It was observed that the late-stage degradation product, phosphoric acid, can significantly influence the osteogenic differentiation of MSCs. In contrast, methionine, which is the early-stage degradation product, showed a minimal influence. Additionally, the study fabricated fiber mats that can lead to enhanced cell alignment while maintaining high porosity. Collectively, this study expanded the applications of PαAPz-M fiber mat protection against oxidative stress and guiding osteogenic differentiation and cell alignment. |
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| ISSN: | 2313-7673 |