Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis
Abstract Disease caused by plasticizers has received increasing attention. Di(2-ethylhexyl) phthalate (DEHP), one of the most widely exposed plasticizers, has been shown to be closely associated with the development of breast cancer (BRCA) in epidemiological studies, but the specific mechanistic tar...
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Nature Portfolio
2025-07-01
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| Online Access: | https://doi.org/10.1038/s41598-025-13201-1 |
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| author | Zhongcheng Wang Yunwen Wang |
| author_facet | Zhongcheng Wang Yunwen Wang |
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| description | Abstract Disease caused by plasticizers has received increasing attention. Di(2-ethylhexyl) phthalate (DEHP), one of the most widely exposed plasticizers, has been shown to be closely associated with the development of breast cancer (BRCA) in epidemiological studies, but the specific mechanistic targets and related pathways are still unclear. In this study, we aimed to elucidate the potential pathogenic targets and mechanisms of DEHP-induced BRCA through network toxicology and molecular docking. Databases including GeneCards, OMIM, ChEMBL, and SwissTargetPrediction were first used to identify DEHP-related targets and BRCA-related targets, and 691 potential targets were obtained from the intersection analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to clarify the biological functions and pathways of potential targets. Protein–protein interaction (PPI) analysis revealed the interactions between potential targets, and 14 hub targets of DEHP-induced BRCA were further screened. To verify the clinical significance of the hub targets, the expression of the target proteins was verified in the TCGA database, and the affinity between DEHP and 12 key targets (hub targets with p < 0.05) was determined via molecular docking. Our study provides a theoretical basis for DEHP-induced BRCA from the “Homo sapiens” perspective and reveals the potential risks caused by exposure to DEHP, thus providing new strategies for the prevention and treatment of DEHP-induced BRCA. |
| format | Article |
| id | doaj-art-f93dd669b58d4cf4b1b42e155b4678a8 |
| institution | Kabale University |
| issn | 2045-2322 |
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| publishDate | 2025-07-01 |
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| spelling | doaj-art-f93dd669b58d4cf4b1b42e155b4678a82025-08-20T03:45:57ZengNature PortfolioScientific Reports2045-23222025-07-0115111210.1038/s41598-025-13201-1Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysisZhongcheng Wang0Yunwen Wang1Department of Pharmacy, The First People’s Hospital of Lin’an District, & Lin’an People’s Hospital Affiliated to HangZhou Medical CollegeDepartment of Pharmacy, The First People’s Hospital of Lin’an District, & Lin’an People’s Hospital Affiliated to HangZhou Medical CollegeAbstract Disease caused by plasticizers has received increasing attention. Di(2-ethylhexyl) phthalate (DEHP), one of the most widely exposed plasticizers, has been shown to be closely associated with the development of breast cancer (BRCA) in epidemiological studies, but the specific mechanistic targets and related pathways are still unclear. In this study, we aimed to elucidate the potential pathogenic targets and mechanisms of DEHP-induced BRCA through network toxicology and molecular docking. Databases including GeneCards, OMIM, ChEMBL, and SwissTargetPrediction were first used to identify DEHP-related targets and BRCA-related targets, and 691 potential targets were obtained from the intersection analysis. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed to clarify the biological functions and pathways of potential targets. Protein–protein interaction (PPI) analysis revealed the interactions between potential targets, and 14 hub targets of DEHP-induced BRCA were further screened. To verify the clinical significance of the hub targets, the expression of the target proteins was verified in the TCGA database, and the affinity between DEHP and 12 key targets (hub targets with p < 0.05) was determined via molecular docking. Our study provides a theoretical basis for DEHP-induced BRCA from the “Homo sapiens” perspective and reveals the potential risks caused by exposure to DEHP, thus providing new strategies for the prevention and treatment of DEHP-induced BRCA.https://doi.org/10.1038/s41598-025-13201-1Breast cancerDi(2-ethylhexyl) phthalateNetwork toxicologyMolecular docking |
| spellingShingle | Zhongcheng Wang Yunwen Wang Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis Scientific Reports Breast cancer Di(2-ethylhexyl) phthalate Network toxicology Molecular docking |
| title | Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis |
| title_full | Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis |
| title_fullStr | Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis |
| title_full_unstemmed | Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis |
| title_short | Mechanism exploration of di(2-ethylhexyl) phthalate (DEHP)-induced breast cancer via network toxicology and molecular docking analysis |
| title_sort | mechanism exploration of di 2 ethylhexyl phthalate dehp induced breast cancer via network toxicology and molecular docking analysis |
| topic | Breast cancer Di(2-ethylhexyl) phthalate Network toxicology Molecular docking |
| url | https://doi.org/10.1038/s41598-025-13201-1 |
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